中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
12期
2858-2860
,共3页
王凤亮%潘国华%钟华%黄强
王鳳亮%潘國華%鐘華%黃彊
왕봉량%반국화%종화%황강
胃癌%趋化因子受体4%CXCL12
胃癌%趨化因子受體4%CXCL12
위암%추화인자수체4%CXCL12
Gastric cancer%CXC chemokine receptor-4%CXCL12
目的 通过检测趋化因子受体4(CXCR4)与趋化因子CXCL12在人胃癌组织中的表达,及其与胃癌临床病理特征的关系.方法 应用免疫组织化学方法检测35例正常胃组织、95例胃癌组织中CXCR4、CXCL12的表达,分析两者与临床病理特征之间的关系.结果 CXCR4蛋白在正常胃组织、胃癌组织、淋巴转移癌、肝脏转移癌、腹膜转移癌的阳性表达率分别为0(0/35)、72.6％(69/95)、89.2％ (69/95)、89.4％ (58/65)和100.0％ (14/14);CXCL12蛋白在正常胃组织、胃癌组织、淋巴转移癌、肝脏转移癌、腹膜转移癌的阳性表达率分别为48.6％(17/35)、84.2％(72/95)、69.2％(45/65)、52.6％(10/19)、35.7％ (4/14).胃癌CXCR4、CXCL12蛋白表达与肿瘤大体形态、浸润深度、淋巴管和血管的侵袭、淋巴结、肝脏和腹膜转移明显相关,而与年龄、性别、肿瘤位置和肿瘤大小无明显相关.胃癌组织中CXCR4与CXCL12蛋白表达呈正相关(r=0.509,P＜0.05).结论 CXCR4和CXCL12的阳性表达与胃癌的发生、增殖、生长及转移等生物学行为密切相关.
目的 通過檢測趨化因子受體4(CXCR4)與趨化因子CXCL12在人胃癌組織中的錶達,及其與胃癌臨床病理特徵的關繫.方法 應用免疫組織化學方法檢測35例正常胃組織、95例胃癌組織中CXCR4、CXCL12的錶達,分析兩者與臨床病理特徵之間的關繫.結果 CXCR4蛋白在正常胃組織、胃癌組織、淋巴轉移癌、肝髒轉移癌、腹膜轉移癌的暘性錶達率分彆為0(0/35)、72.6％(69/95)、89.2％ (69/95)、89.4％ (58/65)和100.0％ (14/14);CXCL12蛋白在正常胃組織、胃癌組織、淋巴轉移癌、肝髒轉移癌、腹膜轉移癌的暘性錶達率分彆為48.6％(17/35)、84.2％(72/95)、69.2％(45/65)、52.6％(10/19)、35.7％ (4/14).胃癌CXCR4、CXCL12蛋白錶達與腫瘤大體形態、浸潤深度、淋巴管和血管的侵襲、淋巴結、肝髒和腹膜轉移明顯相關,而與年齡、性彆、腫瘤位置和腫瘤大小無明顯相關.胃癌組織中CXCR4與CXCL12蛋白錶達呈正相關(r=0.509,P＜0.05).結論 CXCR4和CXCL12的暘性錶達與胃癌的髮生、增殖、生長及轉移等生物學行為密切相關.
목적 통과검측추화인자수체4(CXCR4)여추화인자CXCL12재인위암조직중적표체,급기여위암림상병리특정적관계.방법 응용면역조직화학방법검측35례정상위조직、95례위암조직중CXCR4、CXCL12적표체,분석량자여림상병리특정지간적관계.결과 CXCR4단백재정상위조직、위암조직、림파전이암、간장전이암、복막전이암적양성표체솔분별위0(0/35)、72.6％(69/95)、89.2％ (69/95)、89.4％ (58/65)화100.0％ (14/14);CXCL12단백재정상위조직、위암조직、림파전이암、간장전이암、복막전이암적양성표체솔분별위48.6％(17/35)、84.2％(72/95)、69.2％(45/65)、52.6％(10/19)、35.7％ (4/14).위암CXCR4、CXCL12단백표체여종류대체형태、침윤심도、림파관화혈관적침습、림파결、간장화복막전이명현상관,이여년령、성별、종류위치화종류대소무명현상관.위암조직중CXCR4여CXCL12단백표체정정상관(r=0.509,P＜0.05).결론 CXCR4화CXCL12적양성표체여위암적발생、증식、생장급전이등생물학행위밀절상관.
Objective To investigate the expression of chemokine CXCL12 and its receptor CXC chemokine receptor-4 (CXCR4) protein in gastric cancer and their correlation with several clinicopathological factors.Methods Tirty-five cases of normal gastric tissues and 95 cases of gastric cancer tissues were examined with immunohistochemistry to detect the expression of CXCR4 and CXCL12.The correlation between the CXCR4 and CXCL12 expression and clinicopathological factors was analyzed.Results The positive expression rate of CXCR4 protein in gastric tissue,gastric cancer tissue,lymphatic metastasis,liver metastasis and peritoneal metastasis was 0 (0/35),72.6％ (69/95),89.2％ (69/95),89.4％(58/65) and 100.0％ (14/14),respectively.The positive expression rate of CXCL12 protein in gastric tissue,gastric cancer tissue,lymphatic metastasis,liver metastasis and peritoneal metastasis was 48.6％(17/35),84.2％ (72/95),69.2％ (45/65),52.6％ (10/19) and 5.7％ (4/14),respectively.The expression of CXCR4 and CXCL12 was correlated with the tumor general morphology,depth of invasion,tumor' s invasion on lymphatic nodes and vascular vessels and metastasis of lymph node,liver and peritoneum,but not with age,sex,location of the tumor and tumor size.The expression of CXCL12 was significantly correlated with CXCR4 (r =0.509,P ＜ 0.05).Conclusion CXCR4 /CXCL12 in gastric cancer tissues presented high expression level,along with occurrence,progression and metastasis of gastric cancer.The biological axis may become an important molecular marker to cooperate with gastric cancer progression,invasion and metastasis,and indicate poor prognosis.
