中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2014年
12期
2873-2875
,共3页
宋琦%李传伟%冯晓冬%崔菲菲%彭志海%王学春
宋琦%李傳偉%馮曉鼕%崔菲菲%彭誌海%王學春
송기%리전위%풍효동%최비비%팽지해%왕학춘
结肠癌%非转移性细胞-5
結腸癌%非轉移性細胞-5
결장암%비전이성세포-5
Colon cancer%Non-metastatic cells 5
目的 探讨非转移性细胞-5(NME5)基因在结肠癌发生发展中的意义.方法 应用实时荧光定量聚合酶链反应(Real-time PCR)检测35例结肠癌患者肿瘤组织及癌旁组织NME5基因mRNA的表达水平,通过Western blot以及免疫组织化学法分别检测4例及64例结肠癌患者肿瘤组织及癌旁组织NME5的蛋白表达水平.结果 Real-time PCR结果显示,35例样本中19例(54.3%)样本的癌旁组织中NME5 mRNA的表达水平是结肠癌组织的2倍以上,癌旁组织中NME5mRNA的表达水平(14.61 ±2.63)显著高于结肠癌组织(17.86±1.72,P<0.05).Western blot结果 显示,4例样本中3例样本的癌旁组织NME5蛋白表达明显高于肿瘤组织.免疫组织化学结果显示,NME5蛋白在正常组织中高表达[70.31% (45/64)],在肿瘤组织中无表达或弱表达[82.81%(53/64)].NME5的表达水平与肿瘤分期(P<0.01)和分化(P<0.01)呈高度负相关.结论 NME5基因在正常组织及早期分化良好的结肠癌组织中较高表达,可能参与抑制结肠癌的发生发展.
目的 探討非轉移性細胞-5(NME5)基因在結腸癌髮生髮展中的意義.方法 應用實時熒光定量聚閤酶鏈反應(Real-time PCR)檢測35例結腸癌患者腫瘤組織及癌徬組織NME5基因mRNA的錶達水平,通過Western blot以及免疫組織化學法分彆檢測4例及64例結腸癌患者腫瘤組織及癌徬組織NME5的蛋白錶達水平.結果 Real-time PCR結果顯示,35例樣本中19例(54.3%)樣本的癌徬組織中NME5 mRNA的錶達水平是結腸癌組織的2倍以上,癌徬組織中NME5mRNA的錶達水平(14.61 ±2.63)顯著高于結腸癌組織(17.86±1.72,P<0.05).Western blot結果 顯示,4例樣本中3例樣本的癌徬組織NME5蛋白錶達明顯高于腫瘤組織.免疫組織化學結果顯示,NME5蛋白在正常組織中高錶達[70.31% (45/64)],在腫瘤組織中無錶達或弱錶達[82.81%(53/64)].NME5的錶達水平與腫瘤分期(P<0.01)和分化(P<0.01)呈高度負相關.結論 NME5基因在正常組織及早期分化良好的結腸癌組織中較高錶達,可能參與抑製結腸癌的髮生髮展.
목적 탐토비전이성세포-5(NME5)기인재결장암발생발전중적의의.방법 응용실시형광정량취합매련반응(Real-time PCR)검측35례결장암환자종류조직급암방조직NME5기인mRNA적표체수평,통과Western blot이급면역조직화학법분별검측4례급64례결장암환자종류조직급암방조직NME5적단백표체수평.결과 Real-time PCR결과현시,35례양본중19례(54.3%)양본적암방조직중NME5 mRNA적표체수평시결장암조직적2배이상,암방조직중NME5mRNA적표체수평(14.61 ±2.63)현저고우결장암조직(17.86±1.72,P<0.05).Western blot결과 현시,4례양본중3례양본적암방조직NME5단백표체명현고우종류조직.면역조직화학결과현시,NME5단백재정상조직중고표체[70.31% (45/64)],재종류조직중무표체혹약표체[82.81%(53/64)].NME5적표체수평여종류분기(P<0.01)화분화(P<0.01)정고도부상관.결론 NME5기인재정상조직급조기분화량호적결장암조직중교고표체,가능삼여억제결장암적발생발전.
Objective To investigate the expression of non-metastatic cells 5 (NME5) gene in colon cancer and to explore its potential role in carcinogenesis and progression of colon cancer.Methods Real-time fluorogenic quantitative polymerase chain reaction (Real-time PCR) was conducted to quantify the expression level of NME5 mRNA in the normal and cancerous tissues from 35 patients with colon cancer.Western blotting analysis and immunohistochemistry was conducted to examine the expression level of NME5 protein in paired normal and cancerous tissues from 4 or 64 patients with colon cancer.Results The results shows that among the 35 paired specimens,19 (54.3%) showed at least a 2-fold increase in NME5 mRNA levels in non-cancerous tissues as compared with those in the colon cancerous tissues.The global expression (△Ct) of NME5 was (14.61 ±2.63) in normal tissue and (17.86 ± 1.72) in cancerous tissue respectively.NME5 mRNA expression in normal tissues was significantly higher than that in the cancerous tissues (P <0.05).Western blotting analysis showed that among 4 paired specimens,3 showed significant increase in NME5 protein levels in non-cancerous tissues compared with those in colon cancerous tissues.Immunohistochemical detection of NME5 among 64 paired specimens showed that 70.31% (45/64) of patients highly expressed NME5 in normal tissues,while 82.81% (53/64) of the patients seldom expressed NME5 in cancerous tissues.Low expression of NME5 was significantly associated with advanced clinical stage index (P<0.01) and low differentiation grade (P<0.01).Conclusion The expression of NME5 was highly expressed in normal tissues and well-differentiated cancerous tissues,and it probably participates in inhibiting carcinogenesis and development of colon cancer.