CAJ | 학술논문

目的探讨黏蛋白1(MUC1)在食管鳞癌和正常食管组织中的表达与临床病理特征及预后的关系.方法采用免疫组织化学法检测76例食管鳞癌组织和19例正常食管黏膜组织MUC1的表达;采用x2检验判断其相关性、Kaplan-Meier法进行生存分析、Log-rank检验判断生存差异性、采用Cox比例风险模型进行多因素分析判定独立预后因素.结果 MUC1在正常食管黏膜仅在细胞膜及部分细胞核微弱表达,而在食管鳞癌组织中,MUC1以细胞核表达为主,部分表达于细胞膜,这与MUC1作为一种跨膜糖蛋白在正常组织中的定位不完全相同.MUC1在食管鳞癌组织与正常食管黏膜组织中表达的阳性率分别为72.4％(55/76)和10.5％ (2/19),两者差异有统计学意义(P＜0.01);食管鳞癌患者中,有、无淋巴结转移者的MUC1阳性表达率分别为86.1％(31/36)和60.0％(24/40),两者差异有统计学意义(P＜0.01).伴有MUC1阳性表达患者的5年生存率为16.4％(9/55),阴性表达患者5年生存率为47.6％(10/21),两者差异有统计学意义(P＜0.01).Cox回归分析显示,N分期(P＜0.01)及MUC1的阳性表达(P＜0.05)是独立的预后不良因素.结论 MUC1在食管鳞癌组织中表达增高且以细胞核表达为主,MUC1与淋巴结转移相关.伴有MUC1阳性表达患者的5年生存率明显低于阴性表达患者,MUC1可以作为评估患者预后的分子生物学指标.
목적 탐토점단백1(MUC1)재식관린암화정상식관조직중적표체여림상병리특정급예후적관계.방법 채용면역조직화학법검측76례식관린암조직화19례정상식관점막조직MUC1적표체;채용x2검험판단기상관성、Kaplan-Meier법진행생존분석、Log-rank검험판단생존차이성、채용Cox비례풍험모형진행다인소분석판정독립예후인소.결과 MUC1재정상식관점막부재세포막급부분세포핵미약표체,이재식관린암조직중,MUC1이세포핵표체위주,부분표체우세포막,저여MUC1작위일충과막당단백재정상조직중적정위불완전상동.MUC1재식관린암조직여정상식관점막조직중표체적양성솔분별위72.4％(55/76)화10.5％ (2/19),량자차이유통계학의의(P＜0.01);식관린암환자중,유、무림파결전이자적MUC1양성표체솔분별위86.1％(31/36)화60.0％(24/40),량자차이유통계학의의(P＜0.01).반유MUC1양성표체환자적5년생존솔위16.4％(9/55),음성표체환자5년생존솔위47.6％(10/21),량자차이유통계학의의(P＜0.01).Cox회귀분석현시,N분기(P＜0.01)급MUC1적양성표체(P＜0.05)시독립적예후불량인소.결론 MUC1재식관린암조직중표체증고차이세포핵표체위주,MUC1여림파결전이상관.반유MUC1양성표체환자적5년생존솔명현저우음성표체환자,MUC1가이작위평고환자예후적분자생물학지표.
Objective To investigate the expression of gene Mucin 1 (MUC1) in esophageal squamous cell carcinoma (ESCC) and normal esophageal mucosa tissues,and explore its relationship with clinicopathological characteristics and prognosis.Methods Streptavid-in-peroxidase (SP) immunohistochemistry was used to study the expression of MUC1 in 76 cases of ESCC tissues and 25 cases of normal esophageal mucosa tissues.The survival rate was calculated by Kaplan-meier method and the difference was determined by the log-rank test.The Cox regression analysis was performed to determine the independent risk factors for lymph-metastastic recurrence.Results The expression of MUC1 protein was detected in the cell membrane and part of the nucleus weakly in normal esophageal mucosa tissues,and in the nucleus and part of the cell membrane in ESCC.It did not conform to that MUCI was a kind of transmembrane glycoprotein in the normal tissues.The expression positive rate of MUC1 was 72.4％ (55/76) in ESCC tissues and 10.5％ (2/19) in normal esophageal mucosa tissues with significant difference (P ＜ 0.01).There was a positive correlation between MUC1 positive expression and lymphatic metastasis (P ＜ 0.01).The 5-year survival rate of patients with MUC1 positive expression and without MUC1 positive expression was separately 16.4％ and 47.6％ with significant difference (P ＜0.01).The Cox regression analysis showed that the positive expression of MUC1 (P ＜ 0.05) and the lymphatic metastasis (P ＜ 0.01) were independent risk factors.Conclusion The high expression of MUC1 protein was detected in ESCC and most was in the nucleus.The 5-year survival rate of patients with MUC1 positive expression was significantly lower than that with MUC1 negative patients.The high expression of MUC1 protein is an independent risk factor and can be used as a biomarker of poor prognosis.