CAJ | 학술논문

目的构建自制的脊髓打击仪以及简易棘突间的牵开及稳定装置,探讨利用其建立保留椎板微侵袭小鼠急性脊髓损伤模型的可行性.方法利用电动推杆、压力传感器等制备脊髓打击仪及棘突间的牵开稳定装置;将32只成年雌性KM小鼠随机分成4组,利用上述装置分别以无打击(0 g)、轻度(5 g)、中度(10 g)、重度(20 g)打击力建立保留椎板的小鼠脊髓损伤模型.术后12 h每组随机取2只小鼠行脊髓损伤区苏木素-伊红(HE)染色病理切片,并在术后1、3、7、14d行小鼠后肢运动评分(BBB评分).结果成功制造了脊髓打击仪及简易棘突间牵开稳定装置,并建立了保留椎板的微侵袭小鼠脊髓损伤模型;脊髓损伤的病理学检查结果及后肢运动评分在不同打击力度下差异有统计学意义(P＜0.05).结论应用自制的装置可建立稳定、可靠的保留椎板的微侵袭小鼠脊髓损伤模型,有助于脊髓损伤研究的进一步开展.
목적 구건자제적척수타격의이급간역극돌간적견개급은정장치,탐토이용기건립보류추판미침습소서급성척수손상모형적가행성.방법 이용전동추간、압력전감기등제비척수타격의급극돌간적견개은정장치;장32지성년자성KM소서수궤분성4조,이용상술장치분별이무타격(0 g)、경도(5 g)、중도(10 g)、중도(20 g)타격력건립보류추판적소서척수손상모형.술후12 h매조수궤취2지소서행척수손상구소목소-이홍(HE)염색병리절편,병재술후1、3、7、14d행소서후지운동평분(BBB평분).결과 성공제조료척수타격의급간역극돌간견개은정장치,병건립료보류추판적미침습소서척수손상모형;척수손상적병이학검사결과급후지운동평분재불동타격력도하차이유통계학의의(P＜0.05).결론 응용자제적장치가건립은정、가고적보류추판적미침습소서척수손상모형,유조우척수손상연구적진일보개전.
Objective Try to construct the self-made spinal impactor and the simple interspinous process distraction stabilizing device.Discussion on the feasibility,stability and reliability of method establishing the acute spinal cord injury in mice model retaining the lamina.Methods Using a electric push fool,a pressure sensor and so on,to manufacture the spinal inpactor and the interspinous process distraction stabilizing device.32 adult female rats were randomly divided into four groups,using the self-made spinal impactor device to make varying degrees on the spinal cord of the mice model retaining the lamina,non-combat (0 g),mild (5 g),medium (10 g),and severe (20 g).Taking two mice from every group randomly and making pathological section of damage zone spinal cord after 12 h,testing the behavior BBB grade post operation 1,3 d,7,14 w,in order to evaluate its correlation with the strike force.Results The spinal impactor device and the interspinous process distraction stabilizing device was producted successfully,and the minimally invasive spinal cord injury model retaining the lamina was built.Pathological examination and behavioral score was related with the strike force closely and the grade differences was obvious (P ＜ 0.05).Conclusion A a stable and reliable spinal cord injury model retaining the lamina can be made by the self-made device,and this conducive to the further research on spinal cord injury.