目的 观察跑台运动对轻度脑外伤大鼠空间学习记忆能力及海马脑源性神经营养因子(BDNF)表达的影响.方法 将符合入选标准的30只SD大鼠按随机数字表法分为假手术组(假手术+非运动训练)、运动组(手术+运动训练)、对照组(手术+非运动训练),每组10只.按照Feeney自由落体脑外伤模型制作方法,对运动组和对照组大鼠制作自由落体脑外伤模型,假手术组除不进行自由落体打击外,其他操作同对照组大鼠.运动组大鼠术后第3天(48 h后)按照跑台训练方案开始为期4周运动训练,假手术组和对照组大鼠仅置于跑台上但不转动跑台,运动均安排在每周的第1～5天进行;并于每周的第6天、第7天采用Morris水迷宫对三组大鼠分别进行定位航行实验和空间探索实验,以检测三组大鼠的空间学习记忆能力的变化;运动后第4周Morris水迷宫实验结束后,处死大鼠并取大脑海马组织,采用免疫组织化学方法对大鼠海马CA1区BDNF的阳性细胞进行染色,并测其阳性细胞表达数,以比较大鼠海马中BDNF表达情况.结果 ①定位航行试验中,随着时间的推移,各组大鼠逃避潜伏期均逐渐缩短.与对照组相比,从运动第2周开始,运动组大鼠逃避潜伏期(88.54±5.73 s)已短于对照组(91.45 ±8.91 s)大鼠,至运动第4周,运动组大鼠逃避潜伏期(55.33±6.77 s)较对照组(74.53±6.85 s)明显缩短,差异有统计学意义(P＜0.01);第1～4周,假手术组大鼠逃避潜伏期(88.44±7.79 s,79.52±8.02 s,69.54±10.14 s和62.49±7.22 s)始终短于对照组(98.99±6.84 s,91.45±8.91 s,79.65±12.47 s和74.53±6.85 s),差异有统计学意义(P＜0.01).②空间探索实验中,与对照组相比,假手术组大鼠穿越平台次数(3.00±0.54,3.38±0.74,4.38±1.06和6.00±0.76)明显多于对照组(1.25±0.71,1.50±0.54,2.13±1.25和3.00±0.54),差异有统计学意义(P＜0.01);从第2周起,运动组大鼠穿越平台次数(3.25±1.28,5.00±0.93和5.88±0.99)较对照组(1.50±0.54,2.13±1.25和3.00±0.54)增多,差异有统计学意义(P＜0.01);③免疫组化结果显示,至运动第四周运动组大鼠海马BDNF阳性细胞数(128.56±7.93)分别较对照组(96.38±5.71)和假手术组(94.81±5.49)表达数量增加,差异有统计学意义(P＜0.05),假手术组与对照组比较差异无统计学意义(P＞0.05).结论 跑台运动能够提高轻度脑外伤大鼠的学习和记忆能力,推测其机制可能与海马内BDNF的表达上调有关.
目的 觀察跑檯運動對輕度腦外傷大鼠空間學習記憶能力及海馬腦源性神經營養因子(BDNF)錶達的影響.方法 將符閤入選標準的30隻SD大鼠按隨機數字錶法分為假手術組(假手術+非運動訓練)、運動組(手術+運動訓練)、對照組(手術+非運動訓練),每組10隻.按照Feeney自由落體腦外傷模型製作方法,對運動組和對照組大鼠製作自由落體腦外傷模型,假手術組除不進行自由落體打擊外,其他操作同對照組大鼠.運動組大鼠術後第3天(48 h後)按照跑檯訓練方案開始為期4週運動訓練,假手術組和對照組大鼠僅置于跑檯上但不轉動跑檯,運動均安排在每週的第1～5天進行;併于每週的第6天、第7天採用Morris水迷宮對三組大鼠分彆進行定位航行實驗和空間探索實驗,以檢測三組大鼠的空間學習記憶能力的變化;運動後第4週Morris水迷宮實驗結束後,處死大鼠併取大腦海馬組織,採用免疫組織化學方法對大鼠海馬CA1區BDNF的暘性細胞進行染色,併測其暘性細胞錶達數,以比較大鼠海馬中BDNF錶達情況.結果 ①定位航行試驗中,隨著時間的推移,各組大鼠逃避潛伏期均逐漸縮短.與對照組相比,從運動第2週開始,運動組大鼠逃避潛伏期(88.54±5.73 s)已短于對照組(91.45 ±8.91 s)大鼠,至運動第4週,運動組大鼠逃避潛伏期(55.33±6.77 s)較對照組(74.53±6.85 s)明顯縮短,差異有統計學意義(P＜0.01);第1～4週,假手術組大鼠逃避潛伏期(88.44±7.79 s,79.52±8.02 s,69.54±10.14 s和62.49±7.22 s)始終短于對照組(98.99±6.84 s,91.45±8.91 s,79.65±12.47 s和74.53±6.85 s),差異有統計學意義(P＜0.01).②空間探索實驗中,與對照組相比,假手術組大鼠穿越平檯次數(3.00±0.54,3.38±0.74,4.38±1.06和6.00±0.76)明顯多于對照組(1.25±0.71,1.50±0.54,2.13±1.25和3.00±0.54),差異有統計學意義(P＜0.01);從第2週起,運動組大鼠穿越平檯次數(3.25±1.28,5.00±0.93和5.88±0.99)較對照組(1.50±0.54,2.13±1.25和3.00±0.54)增多,差異有統計學意義(P＜0.01);③免疫組化結果顯示,至運動第四週運動組大鼠海馬BDNF暘性細胞數(128.56±7.93)分彆較對照組(96.38±5.71)和假手術組(94.81±5.49)錶達數量增加,差異有統計學意義(P＜0.05),假手術組與對照組比較差異無統計學意義(P＞0.05).結論 跑檯運動能夠提高輕度腦外傷大鼠的學習和記憶能力,推測其機製可能與海馬內BDNF的錶達上調有關.
목적 관찰포태운동대경도뇌외상대서공간학습기억능력급해마뇌원성신경영양인자(BDNF)표체적영향.방법 장부합입선표준적30지SD대서안수궤수자표법분위가수술조(가수술+비운동훈련)、운동조(수술+운동훈련)、대조조(수술+비운동훈련),매조10지.안조Feeney자유락체뇌외상모형제작방법,대운동조화대조조대서제작자유락체뇌외상모형,가수술조제불진행자유락체타격외,기타조작동대조조대서.운동조대서술후제3천(48 h후)안조포태훈련방안개시위기4주운동훈련,가수술조화대조조대서부치우포태상단불전동포태,운동균안배재매주적제1～5천진행;병우매주적제6천、제7천채용Morris수미궁대삼조대서분별진행정위항행실험화공간탐색실험,이검측삼조대서적공간학습기억능력적변화;운동후제4주Morris수미궁실험결속후,처사대서병취대뇌해마조직,채용면역조직화학방법대대서해마CA1구BDNF적양성세포진행염색,병측기양성세포표체수,이비교대서해마중BDNF표체정황.결과 ①정위항행시험중,수착시간적추이,각조대서도피잠복기균축점축단.여대조조상비,종운동제2주개시,운동조대서도피잠복기(88.54±5.73 s)이단우대조조(91.45 ±8.91 s)대서,지운동제4주,운동조대서도피잠복기(55.33±6.77 s)교대조조(74.53±6.85 s)명현축단,차이유통계학의의(P＜0.01);제1～4주,가수술조대서도피잠복기(88.44±7.79 s,79.52±8.02 s,69.54±10.14 s화62.49±7.22 s)시종단우대조조(98.99±6.84 s,91.45±8.91 s,79.65±12.47 s화74.53±6.85 s),차이유통계학의의(P＜0.01).②공간탐색실험중,여대조조상비,가수술조대서천월평태차수(3.00±0.54,3.38±0.74,4.38±1.06화6.00±0.76)명현다우대조조(1.25±0.71,1.50±0.54,2.13±1.25화3.00±0.54),차이유통계학의의(P＜0.01);종제2주기,운동조대서천월평태차수(3.25±1.28,5.00±0.93화5.88±0.99)교대조조(1.50±0.54,2.13±1.25화3.00±0.54)증다,차이유통계학의의(P＜0.01);③면역조화결과현시,지운동제사주운동조대서해마BDNF양성세포수(128.56±7.93)분별교대조조(96.38±5.71)화가수술조(94.81±5.49)표체수량증가,차이유통계학의의(P＜0.05),가수술조여대조조비교차이무통계학의의(P＞0.05).결론 포태운동능구제고경도뇌외상대서적학습화기억능력,추측기궤제가능여해마내BDNF적표체상조유관.
Objective To investigate the effects of treadmill exercise on spatial learning,memory and the expression of hippocampal brain-derived neurotrophic factor(BDNF) in rats after mild traumatic brain injury (TBI).Methods Thirty Sprague-Dawley rats were randomly divided into group T (n =10,undergoing TBI modelling operation and 4 weeks' treadmill exercise training beginning at 48 h after the operation),group C (n =10,undergoing TBI modelling operation and no exercise training) and group N (n =10,undergoing sham operation and no exercise training).Morris water maze(MWM)was used to test rats' abilities of spatial learning and memory.The number of BDNF-positive cells in the hippocampus was measured with immunohistochemistry.Results In the orientational navigation test,the escape latency period was gradually shortened for rats in all groups.At the second training week,the average time of group T(88.54 ±5.73 s) became shorter than that in group C (91.45 ± 8.91 s).At the fourth week,however,the difference [(55.33 ± 6.77 s) vs (74.53 ± 6.85 s)] was significant(P ＜ 0.01).Group N (88.44 ±7.79 s,79.52 ± 8.02 s,69.54 ± 10.14 s and 62.49 ± 7.22 s respectively) also improved significantly more than group C (98.99 ± 6.84 s,91.45 ± 8.91 s,79.65 ± 12.47 s and 74.53 ± 6.85 s respectively) from week 1 to 4 (P ＜ 0.01).The average number of times of passing through the platform area of group N in four weeks (3.00 ± 0.54,3.38 ± 0.74,4.38 ± 1.06 and 6.00 ± 0.76 respectively) and group T from the second week on(3.25 ± 1.28,5.00 ± 0.93 and 5.88 ± 0.99 respectively) were significantly higher than that of group C (1.25 ± 0.71,1.50 ± 0.54,2.13 ± 1.25 and 3.00 ± 0.54 respectively) accordingly (P ＜ 0.01).At the week four,the average number of BDNF-positive cells in group T(128.56 ± 7.93) were significantly larger than that in group C and group N (P ＜ 0.05),with no significant difference was noted between group N and group C (P ＞0.05).Conclusions Treadmill exercise could improve the spatial learning and memory of rats after mild traumatic brain injury.This may be related with the up-regulation of expression of BDNF in the hippocampus.
