CAJ | 학술논문

目的观察红景天对模拟不同海拔高度缺氧的大鼠视网膜组织形态以及缺氧诱导因子(HIF)表达的影响.方法成年雌性Sprague Dawley大鼠48只随机分为干预组和对照组,每组24只.干预组大鼠按10 ml/lg的剂量腹腔注射大珠红景天注射液,对照组大鼠按相同剂量注射生理盐水.注射后1h,两组随机选取6只大鼠分别饲养于氧分压为17.6、14.7、11.3、7.4 kPa的高原环境模拟实验舱,室内模拟的海拔高度分别为1500、3000、5000、8000 m.饲养后6h摘除大鼠眼球作石蜡切片.采用苏木精伊红染色观察大鼠视网膜病理形态的改变,免疫组织化学染色观察大鼠视网膜中HIF-1α、p53的表达.分析HIF-1α与p53表达的相关性.结果对照组大鼠视网膜各层组织水肿疏松,视网膜厚度增加,层次排列紊乱,神经节细胞肿胀变性,可见核固缩溶解及细胞数量减少;随着模拟的海拔高度增加,其视网膜病理形态改变越明显.干预组大鼠视网膜组织形态改变特点同对照组,改变程度轻于对照组.对照组大鼠视网膜中HIF-1α、p53表达主要位于神经节细胞层和内核层;随着模拟的海拔高度增加,HIF-1α、p53表达也增加.与对照组比较,干预组大鼠视网膜中HIF-1α表达增加,p53表达降低,差异均有统计学意义(P＜0.05).相关性分析结果显示,HIF-1α与p53的表达呈正相关(r=0.984 6,P＜0.05).结论红景天能改善模拟高海拔缺氧所致的大鼠视网膜组织病理损害,其作用机制可能与上调HIF-1α表达、下调p53表达有关.
목적 관찰홍경천대모의불동해발고도결양적대서시망막조직형태이급결양유도인자(HIF)표체적영향.방법 성년자성Sprague Dawley대서48지수궤분위간예조화대조조,매조24지.간예조대서안10 ml/lg적제량복강주사대주홍경천주사액,대조조대서안상동제량주사생리염수.주사후1h,량조수궤선취6지대서분별사양우양분압위17.6、14.7、11.3、7.4 kPa적고원배경모의실험창,실내모의적해발고도분별위1500、3000、5000、8000 m.사양후6h적제대서안구작석사절편.채용소목정이홍염색관찰대서시망막병리형태적개변,면역조직화학염색관찰대서시망막중HIF-1α、p53적표체.분석HIF-1α여p53표체적상관성.결과 대조조대서시망막각층조직수종소송,시망막후도증가,층차배렬문란,신경절세포종창변성,가견핵고축용해급세포수량감소;수착모의적해발고도증가,기시망막병리형태개변월명현.간예조대서시망막조직형태개변특점동대조조,개변정도경우대조조.대조조대서시망막중HIF-1α、p53표체주요위우신경절세포층화내핵층;수착모의적해발고도증가,HIF-1α、p53표체야증가.여대조조비교,간예조대서시망막중HIF-1α표체증가,p53표체강저,차이균유통계학의의(P＜0.05).상관성분석결과현시,HIF-1α여p53적표체정정상관(r=0.984 6,P＜0.05).결론 홍경천능개선모의고해발결양소치적대서시망막조직병리손해,기작용궤제가능여상조HIF-1α표체、하조p53표체유관.
Objective To observe the effects of Rhodiola on the rat retinal tissue morphology and the hypoxia-inducible factor (HIF)-1α at simulated hypoxia at different altitudes.Methods Forty-eight adult female Sprague Dawley rats were randomly divided into the Rhodiola Intervention group (intervention group) and the control group,each group had 24 rats.The intervention group rats were treated with intraperitoneal injection of 10 ml/kg of large plants Rhodiola solution,and the control group rats were injected with same volume of saline.One hour after the injection,six rats were randomly selected from both of the two groups and reared in the plateau environment simulation laboratory modules with the oxygen partial pressure of 17.4,14.6,11.3 and 7.4 kPa,which simulated the altitudes of 1500,3000,5000 and 8000 meters indoor respectively.Six hours later the rat eyeballs were harvested for paraffin sections and analyzed by hematoxylin and eosin staining,and immunohistochemical staining to observe the expression of HIF-1α and p53.Results In the control group,the rat retinal layers were edema and loose,the retinal thickness increased,the retinal structure was disorganized,the ganglion cells were swollen and degenerated,and some can observe the karyopyknosis,karyolysis and the reduced cells number.As the altitude increased,the pathological changes of retinal became more obvious.In the intervention group,the characteristics of rat retinal morphology were same with the control group,while the degree of morphology changes was lighter than the control group.HIF-1α and p53 expressed mainly in the ganglion cell layer and inner nuclear layer of rat retina in the control group.As altitude increased,the expression of HIF-1α and p53 were increased too,which was positive correlated (r=0.9846,P＜0.05).Compared with the control group,the rat retinal expression of HIF1α increased,while expression of p53 decreased in the intervention group,and the differences were statistically significant (P＜0.05).Conclusion Rhodiola can reduce the retinal tissue pathology damage caused by high altitude hypoxia,and its mechanism may be related to the increasing expression of HIF-1α and reducing expression of p53.