中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2014年
42期
3329-3333
,共5页
李静波%林静娜%宋轶萱%相莉%毋中明
李靜波%林靜娜%宋軼萱%相莉%毌中明
리정파%림정나%송질훤%상리%무중명
糖尿病%心肌病%胰岛素样生长因子-1%纤维化%凋亡
糖尿病%心肌病%胰島素樣生長因子-1%纖維化%凋亡
당뇨병%심기병%이도소양생장인자-1%섬유화%조망
Diabetes%Cardiomyopathy%Insulin-like growth factor 1%Fibrosis%Apoptosis
目的 观察胰岛素样生长因子-1(IGF-1)对糖尿病大鼠心肌纤维化及心肌细胞凋亡的影响,探讨其对糖尿病心肌病的保护作用.方法 大鼠尾静脉注射链脲佐菌素建立糖尿病模型,随机分为糖尿病组、胰岛素治疗组及IGF-1治疗组,同时设立正常对照组.干预10周处死大鼠测定心重指数、血清胰岛素和IGF-1水平.取部分左心室组织HE染色及Masson染色,末端标记法(TUNEL)检测心肌细胞凋亡,免疫组织化学及免疫印迹(Western blot)检测大鼠心肌组织IGF-1受体(IGF-1 R)水平.结果 IGF-1及胰岛素治疗后大鼠心重指数较糖尿病组明显降低.与正常对照组相比,糖尿病组血清IGF-1水平明显下降,IGF-1治疗组血清IGF-1水平明显升高.HE及Masson染色结果显示,与正常对照组比较糖尿病大鼠出现心肌组织病变,IGF-1及胰岛素治疗后大鼠心肌组织病变情况明显改善.TUNEL结果显示IGF-1及胰岛素治疗组心肌细胞凋亡指数较糖尿病模型组明显下降.糖尿病大鼠心肌组织中IGF-1R水平较正常对照组明显增加,IGF-1治疗组较糖尿病模型组明显减低,胰岛素治疗组IGF-i R水平较糖尿病模型组无明显变化.结论 IGF-1治疗能够改善心肌间质纤维化及抑制心肌细胞凋亡,对糖尿病心肌病有治疗作用.
目的 觀察胰島素樣生長因子-1(IGF-1)對糖尿病大鼠心肌纖維化及心肌細胞凋亡的影響,探討其對糖尿病心肌病的保護作用.方法 大鼠尾靜脈註射鏈脲佐菌素建立糖尿病模型,隨機分為糖尿病組、胰島素治療組及IGF-1治療組,同時設立正常對照組.榦預10週處死大鼠測定心重指數、血清胰島素和IGF-1水平.取部分左心室組織HE染色及Masson染色,末耑標記法(TUNEL)檢測心肌細胞凋亡,免疫組織化學及免疫印跡(Western blot)檢測大鼠心肌組織IGF-1受體(IGF-1 R)水平.結果 IGF-1及胰島素治療後大鼠心重指數較糖尿病組明顯降低.與正常對照組相比,糖尿病組血清IGF-1水平明顯下降,IGF-1治療組血清IGF-1水平明顯升高.HE及Masson染色結果顯示,與正常對照組比較糖尿病大鼠齣現心肌組織病變,IGF-1及胰島素治療後大鼠心肌組織病變情況明顯改善.TUNEL結果顯示IGF-1及胰島素治療組心肌細胞凋亡指數較糖尿病模型組明顯下降.糖尿病大鼠心肌組織中IGF-1R水平較正常對照組明顯增加,IGF-1治療組較糖尿病模型組明顯減低,胰島素治療組IGF-i R水平較糖尿病模型組無明顯變化.結論 IGF-1治療能夠改善心肌間質纖維化及抑製心肌細胞凋亡,對糖尿病心肌病有治療作用.
목적 관찰이도소양생장인자-1(IGF-1)대당뇨병대서심기섬유화급심기세포조망적영향,탐토기대당뇨병심기병적보호작용.방법 대서미정맥주사련뇨좌균소건립당뇨병모형,수궤분위당뇨병조、이도소치료조급IGF-1치료조,동시설립정상대조조.간예10주처사대서측정심중지수、혈청이도소화IGF-1수평.취부분좌심실조직HE염색급Masson염색,말단표기법(TUNEL)검측심기세포조망,면역조직화학급면역인적(Western blot)검측대서심기조직IGF-1수체(IGF-1 R)수평.결과 IGF-1급이도소치료후대서심중지수교당뇨병조명현강저.여정상대조조상비,당뇨병조혈청IGF-1수평명현하강,IGF-1치료조혈청IGF-1수평명현승고.HE급Masson염색결과현시,여정상대조조비교당뇨병대서출현심기조직병변,IGF-1급이도소치료후대서심기조직병변정황명현개선.TUNEL결과현시IGF-1급이도소치료조심기세포조망지수교당뇨병모형조명현하강.당뇨병대서심기조직중IGF-1R수평교정상대조조명현증가,IGF-1치료조교당뇨병모형조명현감저,이도소치료조IGF-i R수평교당뇨병모형조무명현변화.결론 IGF-1치료능구개선심기간질섬유화급억제심기세포조망,대당뇨병심기병유치료작용.
Objective To observe the effects of insulin-like growth factor-1 (IGF-1) on myocardial fibrosis and myocardial cell apoptosis in diabetic rats,so as to clarify the potential benefit of IGF-1 on diabetic cardiomyopathy.Methods Diabetic rats were induced by caudal injection of STZ,and the diabetic rats were randomly divided into 3 groups:diabetes group,the insulin treatment group and the IGF-1 treatment group.Normal control(NC) group did not receive any therapy.The experiment lasted 10 weeks and the heart weight was recorded to calculate the heart weight index.The serum insulin and IGF-1 level were determined.HE staining and Masson staining were taken on the left ventricular tissue.The cardiomyocyte apoptosis were measured by methods of terminal-deoxynucleotidyl transferase mediated nick end labeling (TUNEL),the expression levels of IGF-1 R in cardiomyocytes were measured by immunohistochemical and Western blot.Results Compared with diabetes group,the heart weight index of the insulin treatment group and the IGF-1 treatment group were significantly decreased.Serum levels of IGF-1 in diabetes group were significantly decreased compared with NC group,and the levels were significantly increased in IGF-1 treatment group.The results of HE and Masson staining showed that compared with NC group,diabetes rats' myocardial tissue were damaged.Myocardial tissue pathological changes of rats were significantly improved after insulin and IGF-1 treatment.The result of TUNEL indicant cardiomyocyte apoptosis index in diabetes group was obviously increased than the NC group,and that in IGF-1 treatment group were decreased compared with diabetes group.IGF-1R levels in diabetic rats myocardial tissue were rised than the NC group,it was declined in IGF-1 treatment group,but in insulin treatment group the levels had no obvious change.Conclusions IGF-1 can inhibite the myocardial fibrosis and cardiomyocyte apoptosis.It maybe have the possible effect on the treatment of diabetic cardiomyopathy.
