CAJ | 학술논문

目的利用生物信息学方法进一步认识肝癌细胞应对肿瘤坏死因子α(TNF-α)刺激过程中的细胞黏附相关的信号转导网络.方法利用肝癌细胞HepG2应对TNF-α刺激的基因芯片数据、转录因子数据库HTRI以及蛋白-蛋白相互作用数据库HRPD构建了肝癌细胞应对TNF-α刺激过程中的信号转导网络,并利用网络结构和基因功能聚类的分析方法进行了研究.结果发现在信号转导网络中MYC、SP1等基因是信号转导网络中的关键转录因子,信号转导网络中的基因与细胞黏附密切相关,EGFR很可能是肝癌细胞应对TNF-α刺激过程中调控细胞黏附的关键基因.结论 EGFR很可能在TNF-α引起的肝癌细胞迁移过程中发挥了关键作用.
목적 이용생물신식학방법진일보인식간암세포응대종류배사인자α(TNF-α)자격과정중적세포점부상관적신호전도망락.방법 이용간암세포HepG2응대TNF-α자격적기인심편수거、전록인자수거고HTRI이급단백-단백상호작용수거고HRPD구건료간암세포응대TNF-α자격과정중적신호전도망락,병이용망락결구화기인공능취류적분석방법진행료연구.결과 발현재신호전도망락중MYC、SP1등기인시신호전도망락중적관건전록인자,신호전도망락중적기인여세포점부밀절상관,EGFR흔가능시간암세포응대TNF-α자격과정중조공세포점부적관건기인.결론 EGFR흔가능재TNF-α인기적간암세포천이과정중발휘료관건작용.
Objective To systemically explore the cellular adhesion signal transduction network of tumor necrosis factor-alpha (TNF-α)-induced hepatocellular carcinoma cells with bioinformatics tools.Methods Published microarray dataset of TNF-α-induced HepG2,human transcription factor database HTRI and human protein-protein interaction database HPRD were used to construct and analyze the signal transduction network.Results In the signal transduction network,MYC and SP1 were the key nodes of signaling transduction.Several genes from the network were closely related with cellular adhesion.Epidermal growth factor receptor (EGFR) is a possible key gene of effectively regulating cellular adhesion during the induction of TNF-α.Conclusion EGFR is a possible key gene for TNF-α-induced metastasis of hepatocellular carcinoma.