中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2014年
43期
3425-3428
,共4页
白杰%赵元立%窦长武%张占普
白傑%趙元立%竇長武%張佔普
백걸%조원립%두장무%장점보
小凹蛋白-1%血管内皮生长因子%脑动静脉畸形
小凹蛋白-1%血管內皮生長因子%腦動靜脈畸形
소요단백-1%혈관내피생장인자%뇌동정맥기형
Caveolin-1%Vascular endothelial growth factor%Cerebral arteriovenous malformation
目的 研究小凹蛋白(Caveolin)-1在人脑动静脉畸形(AVM)组织中的表达及其在人脑AVM血管生成中的作用.方法 收集北京天坛医院神经外科和内蒙古医科大学附属医院神经外科2013年8月至2014年5月手术切除新鲜AVM组织标本,采用免疫组化和免疫印迹测定Caveolin-1、血管内皮生长因子(VEGF)、VEGF-R2和内皮型一氧化氮合酶(eNOS)的表达,分析Caveolin-1表达与VEGF、VEGF-R2以及eNOS之间的关系.结果 Caveolin-1、VEGF、VEGF-R2和eNOS在人脑AVM中的表达率分别为100%、87.27%、76.36%和85.45%,明显高于正常颞浅动脉;免疫印迹检测出血型脑AVM中Caveolin-1、VEGF、VEGF-R2和eNOS表达高于非出血型AVM (P< 0.05);Caveolin-1表达的高低与VEGF、VEGF-R2和eNOS的表达相一致.结论 Caveolin-1在脑AVMs血管生成中起正调节作用,PI3K/Akt信号通路可能是其发挥作用的主要信号通路.Caveolin-1在脑AVM的发生、进展、出血、退化和术后复发中发挥重要作用.
目的 研究小凹蛋白(Caveolin)-1在人腦動靜脈畸形(AVM)組織中的錶達及其在人腦AVM血管生成中的作用.方法 收集北京天罈醫院神經外科和內矇古醫科大學附屬醫院神經外科2013年8月至2014年5月手術切除新鮮AVM組織標本,採用免疫組化和免疫印跡測定Caveolin-1、血管內皮生長因子(VEGF)、VEGF-R2和內皮型一氧化氮閤酶(eNOS)的錶達,分析Caveolin-1錶達與VEGF、VEGF-R2以及eNOS之間的關繫.結果 Caveolin-1、VEGF、VEGF-R2和eNOS在人腦AVM中的錶達率分彆為100%、87.27%、76.36%和85.45%,明顯高于正常顳淺動脈;免疫印跡檢測齣血型腦AVM中Caveolin-1、VEGF、VEGF-R2和eNOS錶達高于非齣血型AVM (P< 0.05);Caveolin-1錶達的高低與VEGF、VEGF-R2和eNOS的錶達相一緻.結論 Caveolin-1在腦AVMs血管生成中起正調節作用,PI3K/Akt信號通路可能是其髮揮作用的主要信號通路.Caveolin-1在腦AVM的髮生、進展、齣血、退化和術後複髮中髮揮重要作用.
목적 연구소요단백(Caveolin)-1재인뇌동정맥기형(AVM)조직중적표체급기재인뇌AVM혈관생성중적작용.방법 수집북경천단의원신경외과화내몽고의과대학부속의원신경외과2013년8월지2014년5월수술절제신선AVM조직표본,채용면역조화화면역인적측정Caveolin-1、혈관내피생장인자(VEGF)、VEGF-R2화내피형일양화담합매(eNOS)적표체,분석Caveolin-1표체여VEGF、VEGF-R2이급eNOS지간적관계.결과 Caveolin-1、VEGF、VEGF-R2화eNOS재인뇌AVM중적표체솔분별위100%、87.27%、76.36%화85.45%,명현고우정상섭천동맥;면역인적검측출혈형뇌AVM중Caveolin-1、VEGF、VEGF-R2화eNOS표체고우비출혈형AVM (P< 0.05);Caveolin-1표체적고저여VEGF、VEGF-R2화eNOS적표체상일치.결론 Caveolin-1재뇌AVMs혈관생성중기정조절작용,PI3K/Akt신호통로가능시기발휘작용적주요신호통로.Caveolin-1재뇌AVM적발생、진전、출혈、퇴화화술후복발중발휘중요작용.
Objective To explore the expression and role of Caveolin-1 in the angiogenesis of cerebral arteriovenous malformation (AVM).Methods A total of 55 fresh AVM samples at Affiliated Hospital,Inner Mongolia Medical University and Beijing Tiantan Hospital from August 2013 to May 2014 were collected to test the expressions of Caveolin-1,vascular endothelial growth factor (VEGF),vascular endothelial growth factor-receptor 2 (VEGF-R2) and endothelial nitric oxide synthase (eNOS) by immunohistochemistry and Western blot.And the relationship between the expressions of Caveolin-1 and VEGF,VEGF-R2 and eNOS was analysed.Results The expression rates of Caveolin-1,VEGF,VEGF-R2 and eNOS in human AVM were 100%,87.27%,76.36% and 85.45%.And the values were significantly higher than those in normal superficial temporal artery.Western blot showed that the expressions of Caveolin-1,VEGF,VEGF-R2 and eNOS in hemorrahge AVM were higher than those in non-hemorrahge counterpart (P < 0.05).The expressions of Caveolin-1,VEGF,VEGF-R2 and eNOS were consistent.Conclusion Caveolin-1 plays a positive regulatory role in the angiogenesis of AVM through the PI3K/Akt signaling pathway.In cerebral AVM,Caveolin-1 plays an important role in occurrence,progression,hemorrahge and degradation of brain AVM.