CAJ | 학술논문

目的研究WW结构域氧化还原酶(WWOX)和原癌基因C-JUN在瘢痕疙瘩中的表达,探讨其在瘢痕疙瘩形成中的作用及机制.方法应用免疫组织化学SP法结合计算机病理图像分析、Western印迹和逆转录聚合酶链反应(RT-PCR)检测正常皮肤和瘢痕疙瘩组织中WWOX和C-JUN的表达,并对其表达进行统计学分析.结果瘢痕疙瘩中WWOX蛋白的表达定位于成纤维细胞细胞质中,且WWOX蛋白及其mRNA的表达减少,与正常皮肤对照组相比差异有统计学意义(P＜0.05);C-JUN蛋白的表达定位于成纤维细胞细胞核和细胞质中,且C-JUN蛋白及其mRNA的表达增多,与正常皮肤对照组相比差异有统计学意义(P＜0.05).两者的表达存在负相关性(r=-0.626,P＜0.01).结论 WWOX在瘢痕疙瘩中低表达,C-JUN在瘢痕疙瘩中高表达,两者是瘢痕疙瘩相关基因,两者存在明显负相关,是瘢痕疙瘩的形成机制之一.WWOX蛋白可能是C-J UN蛋白表达的抑制因素,两者可能通过成纤维细胞在瘢痕疙瘩的形成过程中发挥着重要作用.
목적 연구WW결구역양화환원매(WWOX)화원암기인C-JUN재반흔흘탑중적표체,탐토기재반흔흘탑형성중적작용급궤제.방법 응용면역조직화학SP법결합계산궤병리도상분석、Western인적화역전록취합매련반응(RT-PCR)검측정상피부화반흔흘탑조직중WWOX화C-JUN적표체,병대기표체진행통계학분석.결과 반흔흘탑중WWOX단백적표체정위우성섬유세포세포질중,차WWOX단백급기mRNA적표체감소,여정상피부대조조상비차이유통계학의의(P＜0.05);C-JUN단백적표체정위우성섬유세포세포핵화세포질중,차C-JUN단백급기mRNA적표체증다,여정상피부대조조상비차이유통계학의의(P＜0.05).량자적표체존재부상관성(r=-0.626,P＜0.01).결론 WWOX재반흔흘탑중저표체,C-JUN재반흔흘탑중고표체,량자시반흔흘탑상관기인,량자존재명현부상관,시반흔흘탑적형성궤제지일.WWOX단백가능시C-J UN단백표체적억제인소,량자가능통과성섬유세포재반흔흘탑적형성과정중발휘착중요작용.
Objective To study the expression of WWOX and C-JUN in keloid and to approach their role and mechanism in the pathogenesis of keloid.Methods Immunohistochemical SP methods were used with computer pathological image analysis.Western blot and reverse transcription polymerase chain reaction (RT-RCR) were performed to detect the expression of WWOX and C-JUN in keloid and normal skin with statistical analysis.Results In keloid,the expression of WWOX protein was located in the cytoplasm of fibroblasts,and the expression of WWOX protein and its mRNA decreased,with significantly statistical difference (P＜0.05) compared to normal skin in the control group; the expression of C-JUN protein was located in the cell nucleus and cytoplasm of fibroblasts,with increased expression of C-JUN protein and its mRNA,with significantly statistical difference (P＜0.05) in comparison to normal skin in the control group.The expression of both was negative correlation (r=-0.626,P＜0.01).Conclusions Both WWOX with low expression and C-JUN with high expression are keloid-related genes,having significantly negative correlation between them,which may be one of the mechanisms for the keloid formation.It indicates that the WWOX protein may be an inhibitory factor to the expression of C-JUN protein,and the genes may play a major role in the pathogenesis of keloiod through fibroblasts.