中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2014年
11期
834-838
,共5页
华兴%黄笑笑%廖泽晓%冼其%于莉娜
華興%黃笑笑%廖澤曉%冼其%于莉娜
화흥%황소소%료택효%승기%우리나
乳腺肿瘤%肿瘤侵润%成纤维细胞%免疫表型分型
乳腺腫瘤%腫瘤侵潤%成纖維細胞%免疫錶型分型
유선종류%종류침윤%성섬유세포%면역표형분형
Breast neoplasms%Neoplasm invasiveness%Fibroblasts%Immunophenotyping
目的 探讨在乳腺疾病谱系发展过程中间质成纤维细胞免疫表型的转化及其临床意义.方法 采用免疫组织化学方法分别检测60例正常乳腺、46例乳腺导管上皮细胞不典型增生(ADH)、60例乳腺导管内癌(DCIS)、47例乳腺导管内癌微浸润(DCIS-MI)和60例乳腺浸润性导管癌(IDC)组织中间质成纤维细胞成纤维细胞激活蛋白α(FAP-α)、α-平滑肌肌动蛋白(α-SMA)和CD34的表达.结果 正常乳腺组织中间质成纤维细胞的CD34、FAP-α和α-SMA阳性表达率分别为93.3％、6.7％和18.3％,ADH组织中间质成纤维细胞的CD34、FAP-α和α-SMA阳性表达率分别为95.7％、4.3％和10.9％,DCIS组织中间质成纤维细胞的CD34、FAP-α和α-SMA阳性表达率分别为95.0％、8.3％和15.0％,IDC组织中间质成纤维细胞的CD34、FAP-α和α-SMA阳性表达率分别为35.0％、85.0％和93.3％.正常乳腺、ADH和DCIS组织中间质成纤维细胞的CD34、α-SMA和FAP-α表达差异无统计学意义(x2=1.142,P=0.896),正常乳腺、ADH和DCIS组织的间质成纤维细胞免疫表型主要为CD34+ α-SMA-FAP-α-.IDC与正常乳腺、ADH和DCIS组织中间质成纤维细胞的CD34、α-SMA和FAP-α表达差异有统计学意义(x2=8.351,P＜0.001),IDC和DCIS-MI组织中间质成纤维细胞的免疫表型为CD34-α-SMA+ FAP-α+.结论 间质成纤维细胞CD34表型的丢失及α-SMA和FAP-α表型的获得可能是DCIS-MI浸润前沿间质改变的一项敏感指标,检测DCIS间质成纤维细胞免疫表型的转化将有助于判断DCIS是否已有微浸润,提高DCIS-MI诊断的准确率.
目的 探討在乳腺疾病譜繫髮展過程中間質成纖維細胞免疫錶型的轉化及其臨床意義.方法 採用免疫組織化學方法分彆檢測60例正常乳腺、46例乳腺導管上皮細胞不典型增生(ADH)、60例乳腺導管內癌(DCIS)、47例乳腺導管內癌微浸潤(DCIS-MI)和60例乳腺浸潤性導管癌(IDC)組織中間質成纖維細胞成纖維細胞激活蛋白α(FAP-α)、α-平滑肌肌動蛋白(α-SMA)和CD34的錶達.結果 正常乳腺組織中間質成纖維細胞的CD34、FAP-α和α-SMA暘性錶達率分彆為93.3％、6.7％和18.3％,ADH組織中間質成纖維細胞的CD34、FAP-α和α-SMA暘性錶達率分彆為95.7％、4.3％和10.9％,DCIS組織中間質成纖維細胞的CD34、FAP-α和α-SMA暘性錶達率分彆為95.0％、8.3％和15.0％,IDC組織中間質成纖維細胞的CD34、FAP-α和α-SMA暘性錶達率分彆為35.0％、85.0％和93.3％.正常乳腺、ADH和DCIS組織中間質成纖維細胞的CD34、α-SMA和FAP-α錶達差異無統計學意義(x2=1.142,P=0.896),正常乳腺、ADH和DCIS組織的間質成纖維細胞免疫錶型主要為CD34+ α-SMA-FAP-α-.IDC與正常乳腺、ADH和DCIS組織中間質成纖維細胞的CD34、α-SMA和FAP-α錶達差異有統計學意義(x2=8.351,P＜0.001),IDC和DCIS-MI組織中間質成纖維細胞的免疫錶型為CD34-α-SMA+ FAP-α+.結論 間質成纖維細胞CD34錶型的丟失及α-SMA和FAP-α錶型的穫得可能是DCIS-MI浸潤前沿間質改變的一項敏感指標,檢測DCIS間質成纖維細胞免疫錶型的轉化將有助于判斷DCIS是否已有微浸潤,提高DCIS-MI診斷的準確率.
목적 탐토재유선질병보계발전과정중간질성섬유세포면역표형적전화급기림상의의.방법 채용면역조직화학방법분별검측60례정상유선、46례유선도관상피세포불전형증생(ADH)、60례유선도관내암(DCIS)、47례유선도관내암미침윤(DCIS-MI)화60례유선침윤성도관암(IDC)조직중간질성섬유세포성섬유세포격활단백α(FAP-α)、α-평활기기동단백(α-SMA)화CD34적표체.결과 정상유선조직중간질성섬유세포적CD34、FAP-α화α-SMA양성표체솔분별위93.3％、6.7％화18.3％,ADH조직중간질성섬유세포적CD34、FAP-α화α-SMA양성표체솔분별위95.7％、4.3％화10.9％,DCIS조직중간질성섬유세포적CD34、FAP-α화α-SMA양성표체솔분별위95.0％、8.3％화15.0％,IDC조직중간질성섬유세포적CD34、FAP-α화α-SMA양성표체솔분별위35.0％、85.0％화93.3％.정상유선、ADH화DCIS조직중간질성섬유세포적CD34、α-SMA화FAP-α표체차이무통계학의의(x2=1.142,P=0.896),정상유선、ADH화DCIS조직적간질성섬유세포면역표형주요위CD34+ α-SMA-FAP-α-.IDC여정상유선、ADH화DCIS조직중간질성섬유세포적CD34、α-SMA화FAP-α표체차이유통계학의의(x2=8.351,P＜0.001),IDC화DCIS-MI조직중간질성섬유세포적면역표형위CD34-α-SMA+ FAP-α+.결론 간질성섬유세포CD34표형적주실급α-SMA화FAP-α표형적획득가능시DCIS-MI침윤전연간질개변적일항민감지표,검측DCIS간질성섬유세포면역표형적전화장유조우판단DCIS시부이유미침윤,제고DCIS-MI진단적준학솔.
Objective To evaluate the immunophenotype conversion of fibroblasts and its clinical significance in the process of breast tumor stromal remodeling.Methods CD34,FAP-α,p63 and a-SMA were detected by immunohistochemistry in 273 breast biopsies,including 60 normal breast tissues,46 atypical ductal hyperplasia (ADH),60 ductal carcinoma in situ (DCIS),47 DCIS microinvasive carcinoma (DCIS-MI) and 60 invasive ductal carcinoma (IDC).Results The positive expression rates of CD34,FAP-α and α-SMA in the stromal fibroblasts of normal breast tissues were 93.3％,6.7％ and 18.3％,respectively.Those in the stromal fibroblasts of ADH tissues were 95.7％,4.3％ and 10.9％,respectively.Those in the stromal fibroblasts of DCIS tissues were 95.0％,8.3％ and 15.0％,respectively.Those in the IDC tissues were 35.0％,85.0％ and 93.3％,respectively.The expressions of CD34,α-SMA and FAP-α in the stromal fibroblasts of normal,ASH and DCIS breast tissues did not show significant differences (x2 =1.142,P =0.896).The main immunophenotype of stromal fibroblasts in the tumor-host interface at the invasive front of ADH and DCIS lesions was CD34 + α-SMA + FAP-α +.There were statistically significant differences in the expression of CD34,α-SMA and FAP-α between IDC and ADH,DCIS and normal breast tissues (x2 =8.351,P ＜0.001).The immunophenotype of stromal fibroblasts in the IDC and DCIS-MI breast tissues was CD34-α-SMA + FAP-α +.Conclusions Immunophenotype conversion from CD34 + α-SMA-FAP-α-to CD34-α-SMA + FAP-α + may be a sensitive indicator to judge whether DCIS has microinvasion.Detection of the immunophenotype conversion of stromal fibroblasts may be helpful to determine the presence of microinvasion,and to improve the diagnostic accuracy rate of DCIS.
