国际病毒学杂志
國際病毒學雜誌
국제병독학잡지
INTERNATIONAL JOURNAL OF VIROLOGY
2015年
1期
38-41
,共4页
腺病毒E1%人黑色素瘤%靶向性治疗
腺病毒E1%人黑色素瘤%靶嚮性治療
선병독E1%인흑색소류%파향성치료
Adenovirus E1%Melanoma%Targeted therapy
目的 探讨腺病毒E1对人黑色素瘤靶向性治疗效果.方法 PCR克隆获取腺病毒E1区,增强子DNA片段及鼠酪氨酸酶启动子,采用基因重组法进行构建靶向人黑色素瘤的选择复制性腺病毒的穿梭载体;同源重组法获取人黑色素瘤的选择复制性腺病毒E1,腺病毒E1小剂量攻击人黑色素瘤细胞系SK-Mel-I,同时攻击人肝细胞癌细胞系HepG2,对细胞形态发生的改变、细胞存活数进行记录,利用RT-PCR检测E1A表达,证实腺病毒E1特异性复制.结果 PCR检测结果显示,SK-Mel-I对腺病毒E1裂解杀伤敏感,但是对于HepG2不敏感,提示腺病毒E1在人黑色素瘤细胞中可特异地复制.结论 腺病毒E1对人黑色素瘤具有很好的靶向性.
目的 探討腺病毒E1對人黑色素瘤靶嚮性治療效果.方法 PCR剋隆穫取腺病毒E1區,增彊子DNA片段及鼠酪氨痠酶啟動子,採用基因重組法進行構建靶嚮人黑色素瘤的選擇複製性腺病毒的穿梭載體;同源重組法穫取人黑色素瘤的選擇複製性腺病毒E1,腺病毒E1小劑量攻擊人黑色素瘤細胞繫SK-Mel-I,同時攻擊人肝細胞癌細胞繫HepG2,對細胞形態髮生的改變、細胞存活數進行記錄,利用RT-PCR檢測E1A錶達,證實腺病毒E1特異性複製.結果 PCR檢測結果顯示,SK-Mel-I對腺病毒E1裂解殺傷敏感,但是對于HepG2不敏感,提示腺病毒E1在人黑色素瘤細胞中可特異地複製.結論 腺病毒E1對人黑色素瘤具有很好的靶嚮性.
목적 탐토선병독E1대인흑색소류파향성치료효과.방법 PCR극륭획취선병독E1구,증강자DNA편단급서락안산매계동자,채용기인중조법진행구건파향인흑색소류적선택복제성선병독적천사재체;동원중조법획취인흑색소류적선택복제성선병독E1,선병독E1소제량공격인흑색소류세포계SK-Mel-I,동시공격인간세포암세포계HepG2,대세포형태발생적개변、세포존활수진행기록,이용RT-PCR검측E1A표체,증실선병독E1특이성복제.결과 PCR검측결과현시,SK-Mel-I대선병독E1렬해살상민감,단시대우HepG2불민감,제시선병독E1재인흑색소류세포중가특이지복제.결론 선병독E1대인흑색소류구유흔호적파향성.
Objective To investigate the adenovirus E1 mediated targeting treatment on human melanoma.Methods Adenovirus E1 region,enhancer DNA fragment and the murine tyrosinase promoter were obtained by PCR amplication.Replication adenovirus shuttle vector was constructed by gene recombination.Human melanoma cell line SK-Mel-I and human Liver cancer cell line hepG2 were respectively transfected by adenovirus E1.Cell morphology and cell viable count were recorded and E1A expression was validated by RT-PCR method.Results SK-Mel-I was sensitive to the cleavage of adenovirus E1 and HepG2 was not sensitive,which suggested that the adenovirus E1 could replicate in human melanoma cell.Conclusions Adenovirus E1 has good targeting treatment on the human melanoma.
