CAJ | 학술논문

采用MEMS工艺及C-MEMS工艺制备碳微电极，在其上修饰纳米氧化钌RuO x颗粒后，表现出对胰岛素的电催化氧化特性，可实现胰岛素的电化学检测。以此构建的微型电化学胰岛素传感器，具有较好的电化学性能，其检测灵敏度为1 nA/μM，检测限为800 nM。该传感器结合流动注射分析系统，可获得稳定的测量，且所需样品量少，易实现长时、连续检测，具有潜在的临床应用价值，也可构建细胞传感器，在胰腺β细胞分泌胰岛素的机理研究中具有良好的应用前景。
채용MEMS공예급C-MEMS공예제비탄미전겁，재기상수식납미양화조RuO x과립후，표현출대이도소적전최화양화특성，가실현이도소적전화학검측。이차구건적미형전화학이도소전감기，구유교호적전화학성능，기검측령민도위1 nA/μM，검측한위800 nM。해전감기결합류동주사분석계통，가획득은정적측량，차소수양품량소，역실현장시、련속검측，구유잠재적림상응용개치，야가구건세포전감기，재이선β세포분비이도소적궤리연구중구유량호적응용전경。
Insulin detection is significant in clinical study, but the lack of effective detection methods impedes its clinical applications. In this work, the carbon microelectrode fabricated with MEMS (micro-electromechanical system) and C-MEMS (complex micro-electromechanical system) technology is applied for insulin detection. The carbon microelectrodes were fabricated through the pyrolysis of photoresist and showed good electrochemical performance. After electro-deposition of ruthinium oxide nano particles on the electrode surface, the insulin oxidation is electro-catalyzed and its electrochemical detection is realized at 0.7 V. The sensor has a sensitivity of 1 nA/μM for insulin detection, with a detection limit of 800 nM. Electrochemical measurement shows that the sensor has advantages of high sensitivity, fast response and usability for real time on-site detection.