CAJ | 학술논문

对实验确定的168条σ54启动子序列进行保守性分析，获得两个保守的区域?24区域和?12区域，均为最保守的功能元件。选取保守性最大的17个保守位点的三联体频数作为参数，引入伪计数构建位置权重矩阵，对168条σ54启动子进行预测，分别从编码区和汇聚非编码区共选取168条序列组成阴性集。使用Jackknife交叉验证法对模型进行检验，整体准确度达到82.0%，为σ54启动子的理论和实验研究提供新信息。
대실험학정적168조σ54계동자서렬진행보수성분석，획득량개보수적구역?24구역화?12구역，균위최보수적공능원건。선취보수성최대적17개보수위점적삼련체빈수작위삼수，인입위계수구건위치권중구진，대168조σ54계동자진행예측，분별종편마구화회취비편마구공선취168조서렬조성음성집。사용Jackknife교차험증법대모형진행검험，정체준학도체도82.0%，위σ54계동자적이론화실험연구제공신신식。
By analyzing the 168 experimental-confirmed σ54 promoter sequences, two conservative regions that are ?24 and ?12 regions are obtained. The trimer frequency at 17 positions in these conservative regions is selected as inputting parameter. By adding pseudo-count into position weight matrix, the σ54 promoter can be predicted. The 168 negative sequences are extracted from coding regions and convergent intergenic regions. In Jackknife cross-validation, the overall accuracy reaches to 82.0%, suggesting that the model can be further used in the theoretical and experimental study ofσ54 promoter.