CAJ | 학술논문

目的：探讨督脉、夹脊电针对脊髓损伤（SCI）慢性期大鼠的功能康复作用及机制是否有差异。方法采用脊髓打击器（MASCIS Impactor）计算机程控下精确打击制作大鼠 SCI 模型，选取两组不同配穴的电针（大椎命门组、夹脊组）干预大鼠 SCI 慢性期，通过 BBB 评分观察行为学变化，实时荧光定量 PCR、蛋白免疫印迹法检测以观察神经营养因子（BDNF）和神经营养因子3（NT3） mRNA 及蛋白的表达变化，分析大椎命门组和夹脊组的电针干预对 SCI 慢性期神经营养因子表达及功能康复的影响。所有数据采用 SPSS 16.0统计软件进行分析处理，用均值±标准差（x±s）表示。多组间比较采用单因素方差分析，两两比较方差齐时用 LSD-t 法，方差不齐时用 Dunnett 法。以 P<0.05为差异有统计学意义。结果经电针治疗后，BBB 评分显示，与模型组相比，两组电针治疗组在造模后各时段运动能力均逐步提高（P<0.05），夹脊组评分高于大椎命门组（P<0.05）。造模后7周，各组间的 BDNF mRNA 表达量差异有统计学意义（F=451.8，P<0.05）；大椎命门组、夹脊组的 BDNF mRNA 表达水平均高于模型对照组（t=15.2，t=26.8，均 P<0.05）；夹脊组高于大椎命门组（t=11.6， P<0.05）。各组间的 NT-3 mRNA 表达量差异有统计学意义（F=320.4，P<0.05），大椎命门组、夹脊组的 NT-3 mRNA 表达水平均高于模型对照组（t=14.7，t=21.2，均 P<0.05）；且夹脊组高于大椎命门组（t=6.6，P<0.05）。各组间的 BDNF 蛋白表达量差异有统计学意义（F=50.3，P<0.05）；大椎命门组、夹脊组的 BDNF 蛋白表达量高于模型组（t=0.4，t=0.8，均 P<0.05）；夹脊组高于大椎命门组（t=3.6，P<0.05）。各组间的 NT-3蛋白表达量差异有统计学意义（F=39.6，P<0.05）；大椎命门组、夹脊组的 NT-3蛋白表达量高于模型组（t=3.8，t=6.9，均P<0.05），且夹脊组高于大椎命门组（t=3.1， P<0.05）。结论对 SCI 慢性期大鼠，督脉、夹脊电针治疗均通过增加损伤局部脊髓的 BDNF、NT-3的表达，促进的神经修复和功能康复；夹脊组作用强于大椎命门组。目的：探討督脈、夾脊電針對脊髓損傷（SCI）慢性期大鼠的功能康複作用及機製是否有差異。方法採用脊髓打擊器（MASCIS Impactor）計算機程控下精確打擊製作大鼠 SCI 模型，選取兩組不同配穴的電針（大椎命門組、夾脊組）榦預大鼠 SCI 慢性期，通過 BBB 評分觀察行為學變化，實時熒光定量 PCR、蛋白免疫印跡法檢測以觀察神經營養因子（BDNF）和神經營養因子3（NT3） mRNA 及蛋白的錶達變化，分析大椎命門組和夾脊組的電針榦預對 SCI 慢性期神經營養因子錶達及功能康複的影響。所有數據採用 SPSS 16.0統計軟件進行分析處理，用均值±標準差（x±s）錶示。多組間比較採用單因素方差分析，兩兩比較方差齊時用 LSD-t 法，方差不齊時用 Dunnett 法。以 P<0.05為差異有統計學意義。結果經電針治療後，BBB 評分顯示，與模型組相比，兩組電針治療組在造模後各時段運動能力均逐步提高（P<0.05），夾脊組評分高于大椎命門組（P<0.05）。造模後7週，各組間的 BDNF mRNA 錶達量差異有統計學意義（F=451.8，P<0.05）；大椎命門組、夾脊組的 BDNF mRNA 錶達水平均高于模型對照組（t=15.2，t=26.8，均 P<0.05）；夾脊組高于大椎命門組（t=11.6， P<0.05）。各組間的 NT-3 mRNA 錶達量差異有統計學意義（F=320.4，P<0.05），大椎命門組、夾脊組的 NT-3 mRNA 錶達水平均高于模型對照組（t=14.7，t=21.2，均 P<0.05）；且夾脊組高于大椎命門組（t=6.6，P<0.05）。各組間的 BDNF 蛋白錶達量差異有統計學意義（F=50.3，P<0.05）；大椎命門組、夾脊組的 BDNF 蛋白錶達量高于模型組（t=0.4，t=0.8，均 P<0.05）；夾脊組高于大椎命門組（t=3.6，P<0.05）。各組間的 NT-3蛋白錶達量差異有統計學意義（F=39.6，P<0.05）；大椎命門組、夾脊組的 NT-3蛋白錶達量高于模型組（t=3.8，t=6.9，均P<0.05），且夾脊組高于大椎命門組（t=3.1， P<0.05）。結論對 SCI 慢性期大鼠，督脈、夾脊電針治療均通過增加損傷跼部脊髓的 BDNF、NT-3的錶達，促進的神經脩複和功能康複；夾脊組作用彊于大椎命門組。
목적：탐토독맥、협척전침대척수손상（SCI）만성기대서적공능강복작용급궤제시부유차이。방법채용척수타격기（MASCIS Impactor）계산궤정공하정학타격제작대서 SCI 모형，선취량조불동배혈적전침（대추명문조、협척조）간예대서 SCI 만성기，통과 BBB 평분관찰행위학변화，실시형광정량 PCR、단백면역인적법검측이관찰신경영양인자（BDNF）화신경영양인자3（NT3） mRNA 급단백적표체변화，분석대추명문조화협척조적전침간예대 SCI 만성기신경영양인자표체급공능강복적영향。소유수거채용 SPSS 16.0통계연건진행분석처리，용균치±표준차（x±s）표시。다조간비교채용단인소방차분석，량량비교방차제시용 LSD-t 법，방차불제시용 Dunnett 법。이 P<0.05위차이유통계학의의。결과경전침치료후，BBB 평분현시，여모형조상비，량조전침치료조재조모후각시단운동능력균축보제고（P<0.05），협척조평분고우대추명문조（P<0.05）。조모후7주，각조간적 BDNF mRNA 표체량차이유통계학의의（F=451.8，P<0.05）；대추명문조、협척조적 BDNF mRNA 표체수평균고우모형대조조（t=15.2，t=26.8，균 P<0.05）；협척조고우대추명문조（t=11.6， P<0.05）。각조간적 NT-3 mRNA 표체량차이유통계학의의（F=320.4，P<0.05），대추명문조、협척조적 NT-3 mRNA 표체수평균고우모형대조조（t=14.7，t=21.2，균 P<0.05）；차협척조고우대추명문조（t=6.6，P<0.05）。각조간적 BDNF 단백표체량차이유통계학의의（F=50.3，P<0.05）；대추명문조、협척조적 BDNF 단백표체량고우모형조（t=0.4，t=0.8，균 P<0.05）；협척조고우대추명문조（t=3.6，P<0.05）。각조간적 NT-3단백표체량차이유통계학의의（F=39.6，P<0.05）；대추명문조、협척조적 NT-3단백표체량고우모형조（t=3.8，t=6.9，균P<0.05），차협척조고우대추명문조（t=3.1， P<0.05）。결론대 SCI 만성기대서，독맥、협척전침치료균통과증가손상국부척수적 BDNF、NT-3적표체，촉진적신경수복화공능강복；협척조작용강우대추명문조。
SCI rats; RT-qPCR detection and Western-blot to observe the mRNA and protein expression changes of brain derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3); to analyze the effects of EA intervention with different point combinations on function recovery of SCI rats in chronic phase.Statistical analysis was performed using statistical program SPSS 16.0 software . All the data are presented as mean±SD values(x±s). ANOVA for comparing multiple data from different groups was used. For pairwise comparing, if homogeneity of variance was positive, LSD-t method would be choosed,while if was not, Dunnett method would be choosed. P value <0.05 was considered to be significant statistically. Results After treatments of EA with two different point combinations on SCI rats in chronic phase, BBB score at each time point showed gradually increase of locomotion function in each treatment group compared with the model group (P<0.05), but the most significantly increase in Jiaji group (P<0.05). For BDNF mRNA,there was significant difference among every group(F=451.8, P<0.05),and there was increased expression of BDNF mRNA in Dazhui & Mingmen group and Jiaji group ,compared with that in the model group(t=15.2,t=26.8, all P<0.05);an increased expression of BDNF mRNA was detected in Jiaji group compared with that in Dazhui & Mingmen group(t=11.6, P<0.05). For NT-3 mRNA,there was significant difference among every group(F=320.4, P<0.05),and there was increased expression of NT-3 mRNA in Dazhui& Mingmen group and Jiaji group ,compared with that in the model group(t=14.7, t=21.2,all P<0.05);an increased expression of NT-3 mRNA was detected in Jiaji group compared with that in Dazhui & Mingmen group(t=6.6,P<0.05). For BDNF protein,there was significant difference among every group(F=50.3, P<0.05),and there was increased expression of BDNF in Dazhui & Mingmen group and Jiaji group ,compared with that in the model group(t=0.4, t=0.8, all P<0.05);an increased expression of BDNF was detected in Jiaji group compared with that in Dazhui & Mingmen group(t=3.6, P<0.05).For NT-3 protein,there was significant difference among every group(F=39.6, P<0.05),and there was increased expression of NT-3 in Dazhui & Mingmen group and Jiaji group ,compared with that in the model group(t=3.8, t=6.9, all P<0.05);an increased expression of NT-3 was detected in Jiaji group compared with that in Dazhui & Mingmen group(t=3.1, P<0.05). Conclusion EA administrations with different point combinations on SCI rats in chronic phase, greatly promoted neuronal recovery and function rehabilitation by increasing the expression of BDNF and NT-3 in local injury spinal cord, whereas, the Jiaji group had the most remarkable effect.