中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2015年
1期
178-179
,共2页
郑克彬%刘艳峰%刘海鹏%单小松%郭毅%李春晖%李志红
鄭剋彬%劉豔峰%劉海鵬%單小鬆%郭毅%李春暉%李誌紅
정극빈%류염봉%류해붕%단소송%곽의%리춘휘%리지홍
颅脑损伤%基质金属蛋白酶%基质金属蛋白酶抑制剂%脑脊液
顱腦損傷%基質金屬蛋白酶%基質金屬蛋白酶抑製劑%腦脊液
로뇌손상%기질금속단백매%기질금속단백매억제제%뇌척액
Traumatic brain injury%Matrix metalloproteinase%Tissue inhibitor of metalloproteinase%Cerebrospinal fluid
目的 探讨颅脑损伤(TBI)患者基质金属蛋白酶(MMPs)和基质金属蛋白酶抑制剂(TIMPs)水平之间的关系以及与临床预后的相关性.方法 收集颅脑损伤患者30例,并分为轻重两组,分别留取24、72、120 h内脑脊液及静脉血,10例健康体检者为对照组,用双抗体夹心酶联免疫吸附试验(ELISA)法检测MMPs和TIMPs水平.结果 本实验检测到MMP-9在颅脑损伤患者组脑脊液中24 h内达到高峰[(71.34±13.93) μg/L],可以持续72 h.TIMP-1在120 h内可达高峰[(162.24±17.15) μg/L],相对第72小时及第24小时内差异有统计学意义(P＜0.05).颅脑损伤患者与对照组的MMP-9、MMP-2、TIMP-1及TIMP-2无论脑脊液还是血液中的表达差异均有统计学意义(P＜0.05).重度TBI患者组的MMP-9在脑脊液水平显著高于轻度TBI患者组.颅脑损伤患者组伤后MMP-9 (24 h)含量与TIMP-1(120 h)含量呈正相关(P＜0.01).结论 MMPs和TIMPs在颅脑损伤患者脑脊液和血浆中增加,TIMP-1和MMP-9呈正相关,MMP-9可以作为预测颅脑损伤患者临床预后的指标.
目的 探討顱腦損傷(TBI)患者基質金屬蛋白酶(MMPs)和基質金屬蛋白酶抑製劑(TIMPs)水平之間的關繫以及與臨床預後的相關性.方法 收集顱腦損傷患者30例,併分為輕重兩組,分彆留取24、72、120 h內腦脊液及靜脈血,10例健康體檢者為對照組,用雙抗體夾心酶聯免疫吸附試驗(ELISA)法檢測MMPs和TIMPs水平.結果 本實驗檢測到MMP-9在顱腦損傷患者組腦脊液中24 h內達到高峰[(71.34±13.93) μg/L],可以持續72 h.TIMP-1在120 h內可達高峰[(162.24±17.15) μg/L],相對第72小時及第24小時內差異有統計學意義(P＜0.05).顱腦損傷患者與對照組的MMP-9、MMP-2、TIMP-1及TIMP-2無論腦脊液還是血液中的錶達差異均有統計學意義(P＜0.05).重度TBI患者組的MMP-9在腦脊液水平顯著高于輕度TBI患者組.顱腦損傷患者組傷後MMP-9 (24 h)含量與TIMP-1(120 h)含量呈正相關(P＜0.01).結論 MMPs和TIMPs在顱腦損傷患者腦脊液和血漿中增加,TIMP-1和MMP-9呈正相關,MMP-9可以作為預測顱腦損傷患者臨床預後的指標.
목적 탐토로뇌손상(TBI)환자기질금속단백매(MMPs)화기질금속단백매억제제(TIMPs)수평지간적관계이급여림상예후적상관성.방법 수집로뇌손상환자30례,병분위경중량조,분별류취24、72、120 h내뇌척액급정맥혈,10례건강체검자위대조조,용쌍항체협심매련면역흡부시험(ELISA)법검측MMPs화TIMPs수평.결과 본실험검측도MMP-9재로뇌손상환자조뇌척액중24 h내체도고봉[(71.34±13.93) μg/L],가이지속72 h.TIMP-1재120 h내가체고봉[(162.24±17.15) μg/L],상대제72소시급제24소시내차이유통계학의의(P＜0.05).로뇌손상환자여대조조적MMP-9、MMP-2、TIMP-1급TIMP-2무론뇌척액환시혈액중적표체차이균유통계학의의(P＜0.05).중도TBI환자조적MMP-9재뇌척액수평현저고우경도TBI환자조.로뇌손상환자조상후MMP-9 (24 h)함량여TIMP-1(120 h)함량정정상관(P＜0.01).결론 MMPs화TIMPs재로뇌손상환자뇌척액화혈장중증가,TIMP-1화MMP-9정정상관,MMP-9가이작위예측로뇌손상환자림상예후적지표.
Objective In this study,we investigated matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinase (TIMPs) in cerebrospinal fluid (CSF) and plasma of traumatic brain injury (TBI) patients.Methods A total of 30 patients with moderate and severe TBI and 15 age-matched controls were enrolled in this study.Plasma and CSF samples were collected within 24 h (as the initial value),at 72 and 120 h post injury.CSF and plasma MMP-9,MMP-2,TIMP-1 and TIMP-2 were estimated using enzyme linked immunosorbent assay (ELISA).Different levels of these indexes were compared in the two groups and further investigated the correlation between each other.Results There was a significant elevation in the levels of the initial MMP-9 in the CSF [(71.34 ± 13.93) μg/L],which lasted for 72 h post injury.TIMP-1 kept increasing within 120 h post injury[(162.24 ±17.15) μg/L] and it was different compared with TIMP-1 at 24 and 72 h post injury.Plasma levels of MMP-9,MMP-2,TIMP-1 and TIMP-2 in TBI patients were also significantly different from those in controls.Furthermore the CSF MMP-9 in patients with severe TBI was higher than that in patients with moderate TBI.In addition,there was a positive relationship between the initial MMP-9 and TIMP-1 at 120 h post injury (r =0.614,P ＜0.01).Conclusion MMPs and TIMPs are increased in both CSF and plasma of TBI patients.TIMP-1 has a positive correlation with MMP-9 and the initial MMP-9 is associated with the neurological outcomes.
