CAJ | 학술논문

目的观察鞘内注射过表达胶质细胞源性神经生长因子(GDNF)慢病毒载体对慢性缩窄性神经损伤(CCI)大鼠神经病理性疼痛的影响.方法采用结扎大鼠坐骨神经制备CCI模型.CCI造模成功后第7天鞘内注射GDNF过表达慢病毒载体.CCI造模前及造模后第3、5、7、14、21天测定大鼠机械痛阈,并于术后第21天采用Western免疫印迹法测定脊髓GDNF表达.结果 Western免疫印迹显示CCI组GDNF表达较假手术组减少(P＜0.05);鞘内注射GDNF过表达慢病毒载体后,脊髓GDNF表达上调,且CCI大鼠机械痛敏显著降低(P＜0.05).结论上调脊髓GDNF表达可减轻CCI大鼠神经病理性疼痛.
목적 관찰초내주사과표체효질세포원성신경생장인자(GDNF)만병독재체대만성축착성신경손상(CCI)대서신경병이성동통적영향.방법 채용결찰대서좌골신경제비CCI모형.CCI조모성공후제7천초내주사GDNF과표체만병독재체.CCI조모전급조모후제3、5、7、14、21천측정대서궤계통역,병우술후제21천채용Western면역인적법측정척수GDNF표체.결과 Western면역인적현시CCI조GDNF표체교가수술조감소(P＜0.05);초내주사GDNF과표체만병독재체후,척수GDNF표체상조,차CCI대서궤계통민현저강저(P＜0.05).결론 상조척수GDNF표체가감경CCI대서신경병이성동통.
Objective To investigate the effect of intrathecal injection of lentiviral vector-mediated up-regulation of glial cell line-derived neurotrophicfactor (GDNF) on neuropathic pain of chronic constriction injury (CCI) rats.Methods The CCI model was prepared by ligating the sciatic nerve of Sprague-Dawley (SD) rats.Seven days after CCI modeling,a single intrathecal injection of lentiviral vectors (LV)-GDNF was given.Before CCI and 3,5,7,14,and 21 days after CCI modeling,the mechanical pain threshold was tested in rats,and 21 days after surgery,Western blot was used to detect the expression of GDNF protein.Results On 21 days after CCI modeling,GDNF expression was reduced compared to sham group.After intrathecal injection of LV-GDNF,GDNF expression was up-regulated in the spinal cord,and CCI-induced mechanical hyperalgesia in rats was alleviated.Conclusions Intrathecal injection LV-GDNF can up-regulate the expression of GDNF and alleviate neuropathic pain in CCI rats.