中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2015年
2期
300-301
,共2页
潘延凤%郑岩%秦涛%张耀丹%伍孝贤%李花花
潘延鳳%鄭巖%秦濤%張耀丹%伍孝賢%李花花
반연봉%정암%진도%장요단%오효현%리화화
癌,肝细胞%细胞骨架蛋白%UC001kfo%α-平滑肌肌动蛋白
癌,肝細胞%細胞骨架蛋白%UC001kfo%α-平滑肌肌動蛋白
암,간세포%세포골가단백%UC001kfo%α-평활기기동단백
Carcinoma,hepatocellular%Cytoskeleton protein%UC001kfo%α-smooth muscle actin
目的 观察新基因UC001 kfo对肝癌细胞细胞骨架蛋白表达的调控效应.方法 以肝癌细胞株HepG2为细胞模型,采用脂质体转染技术分别将UC001kfo-pCDNA和pCDNA转染至HepG2细胞内,分为UC001 kfo-pCDNA、pCDNA、HepG2组,每组设3个复孔,实时定量聚合酶链反应(Real-time PCR)检测UC001 kfo、α-平滑肌肌动蛋白(α-SMA)mRNA表达,免疫组织化学及积分吸光度(IA)分析α-SMA的表达.结果 Real-time PCR结果显示48 h后UC001kfo表达量在UC001 kfo-pCDNA组、pCDNA组、HepG2组分别为1 924.14±238.69、1.87 ±0.43、1.00±0.29,UC001 kfo-pCDNA组与阴性对照组和空白对照组比较差异有统计学意义(P＜0.01);α-SMA表达量分别为6.81 ±1.39、0.82±0.14、1.00±0.54,UC001 kfo-pCDNA组与阴性对照组和空白对照组比较差异均有统计学意义(P＜0.01).48 h UC001kfo-pCDNA、pCDNA、HepG2组IA值分别为972.53±59.10、623.11±57.66、697.98±51.29,UC001 kfo-pCDNA组与阴性对照组比较差异有统计学意义(P＜0.01),与空白组比较差异无统计学意义(P＞0.05).结论 UC001 kfo上调α-SMA的表达,促进肝癌细胞的侵袭转移.
目的 觀察新基因UC001 kfo對肝癌細胞細胞骨架蛋白錶達的調控效應.方法 以肝癌細胞株HepG2為細胞模型,採用脂質體轉染技術分彆將UC001kfo-pCDNA和pCDNA轉染至HepG2細胞內,分為UC001 kfo-pCDNA、pCDNA、HepG2組,每組設3箇複孔,實時定量聚閤酶鏈反應(Real-time PCR)檢測UC001 kfo、α-平滑肌肌動蛋白(α-SMA)mRNA錶達,免疫組織化學及積分吸光度(IA)分析α-SMA的錶達.結果 Real-time PCR結果顯示48 h後UC001kfo錶達量在UC001 kfo-pCDNA組、pCDNA組、HepG2組分彆為1 924.14±238.69、1.87 ±0.43、1.00±0.29,UC001 kfo-pCDNA組與陰性對照組和空白對照組比較差異有統計學意義(P＜0.01);α-SMA錶達量分彆為6.81 ±1.39、0.82±0.14、1.00±0.54,UC001 kfo-pCDNA組與陰性對照組和空白對照組比較差異均有統計學意義(P＜0.01).48 h UC001kfo-pCDNA、pCDNA、HepG2組IA值分彆為972.53±59.10、623.11±57.66、697.98±51.29,UC001 kfo-pCDNA組與陰性對照組比較差異有統計學意義(P＜0.01),與空白組比較差異無統計學意義(P＞0.05).結論 UC001 kfo上調α-SMA的錶達,促進肝癌細胞的侵襲轉移.
목적 관찰신기인UC001 kfo대간암세포세포골가단백표체적조공효응.방법 이간암세포주HepG2위세포모형,채용지질체전염기술분별장UC001kfo-pCDNA화pCDNA전염지HepG2세포내,분위UC001 kfo-pCDNA、pCDNA、HepG2조,매조설3개복공,실시정량취합매련반응(Real-time PCR)검측UC001 kfo、α-평활기기동단백(α-SMA)mRNA표체,면역조직화학급적분흡광도(IA)분석α-SMA적표체.결과 Real-time PCR결과현시48 h후UC001kfo표체량재UC001 kfo-pCDNA조、pCDNA조、HepG2조분별위1 924.14±238.69、1.87 ±0.43、1.00±0.29,UC001 kfo-pCDNA조여음성대조조화공백대조조비교차이유통계학의의(P＜0.01);α-SMA표체량분별위6.81 ±1.39、0.82±0.14、1.00±0.54,UC001 kfo-pCDNA조여음성대조조화공백대조조비교차이균유통계학의의(P＜0.01).48 h UC001kfo-pCDNA、pCDNA、HepG2조IA치분별위972.53±59.10、623.11±57.66、697.98±51.29,UC001 kfo-pCDNA조여음성대조조비교차이유통계학의의(P＜0.01),여공백조비교차이무통계학의의(P＞0.05).결론 UC001 kfo상조α-SMA적표체,촉진간암세포적침습전이.
Objective To explore the regulatory effect of the new gene UC001kfo on the expression of cytoskeletal protein in hepatocellular carcinoma cells.Methods The hepatocellular carcinoma cell line HepG2 was used as the cell model,the liposomal transfection technique was applied to transfect the UC001kfo-pCDNA and pCDNA into the HepG2 cells respectively,and three groups were set up:UC001kfo-pCDNA group,pCDNA group and HepG2 group (three holes for each group).Real-time quantitative polymerase chain reaction (Real-time PCR) was used to detect the expression of UC001kfo,and α-smooth muscle actin (ot-SMA) mRNA.Immunohistochemistry and the integral absorbance (IA) were used to analyze the expression of α-SMA.Results Real-time PCR showed that the expression of UC001kfo in UC001kfo-pCDNA group,pCDNA group and HepG2 group was 1924.14 ±238.69,1.87 ± 0.43 and 1.00 ± 0.29 respectively,and there was statistically significant difference between UC001 kfo-pCDNA group and pCDNA group or HepG2 group (both P ＜0.01).α-SMA expression in UC001kfo-pCDNA group,pCDNA group and HepG2 group was 6.81 ± 1.39,0.82 ±0.14 and 1.00 ±0.54 respectively,and there was statistically significant difference between UC001kfo-pCDNA group and pCDNA group or HepG2 group (both P ＜0.01).The IA values in UC001kfo-pCDNA group,pCDNA group and HepG2 group were 972.53 ±59.10,623.11 ±57.66 and 697.98 ±51.29 respectively,and there was statistically significant difference between UC001 kfo-pCDNA group and pCDNA group or HepG2 group (P ＜ 0.01),but no statistically significant difference was found between pCDNA group and HepG2 group (P ＞ 0.05).Conclusion UC001 kfo can increase the expression of α-SMA and promote the invasion and migration of hepatocellular carcinoma cells.
