中华物理医学与康复杂志
中華物理醫學與康複雜誌
중화물리의학여강복잡지
CHINESE JOURNAL OF PHYSICAL MEDICINE AND REHABILITATION
2014年
12期
897-901
,共5页
张文亮%蔡颖%谢康玲%李剑%刘遂心
張文亮%蔡穎%謝康玲%李劍%劉遂心
장문량%채영%사강령%리검%류수심
运动%胰岛素抵抗%氧化物酶体增殖物激活受体-α%脂酰辅酶A氧化酶%烯酰辅酶A水解酶/L-3羟-CoA脱氢酶
運動%胰島素牴抗%氧化物酶體增殖物激活受體-α%脂酰輔酶A氧化酶%烯酰輔酶A水解酶/L-3羥-CoA脫氫酶
운동%이도소저항%양화물매체증식물격활수체-α%지선보매A양화매%희선보매A수해매/L-3간-CoA탈경매
Exercises%Insulin resistance%Peroxisome proliferators-activated receptor alpha%acyl-CoA oxidase%Enoyl-CoA-hydratase and 3-hydroxyacyl-CoA-dehydrogenase
目的 观察运动对胰岛素抵抗小鼠骨骼肌过氧化物酶体增殖物激活受体-α(PPAR-α)及靶基因脂酰辅酶A氧化酶(ACO)、烯酰辅酶A水解酶/L-3羟-CoA脱氢酶(EHHADH)mRNA表达的影响,并初步探讨运动改善脂代谢的可能机制.方法 将20只雄性ApoE基因敲除小鼠随机分为高脂组及运动组,高脂组小鼠给予高脂饮食喂养,运动组小鼠在高脂饮食基础上增加游泳训练,另选取C57BL/6J小鼠作为对照组.经干预12周后,测定各组小鼠空腹血脂、血糖及胰岛素浓度,并计算胰岛素抵抗指数;同时取各组小鼠腓肠肌进行电镜超微结构观察,采用RT-PCR方法检测各组小鼠骨骼肌中PPAR-α、ACO及EHHADH mRNA水平.结果 通过电镜观察发现,高脂组可见肌细胞线粒体水肿、肌膜下水肿、肌原纤维间水肿,伴有肌溶解灶,该组小鼠血脂(总胆固醇、低密度脂蛋白、游离脂肪酸)、空腹血糖、空腹胰岛素及胰岛素抵抗指数均较对照组明显升高(均P ＜0.05),骨骼肌中PPAR-α、ACO及EHHADH mRNA表达均较对照组明显下调(均P＜0.05).运动组小鼠经游泳干预后,发现其肌细胞肌原纤维间有轻度水肿,肌纤维排列整齐,未见肌溶解及肌膜下水肿;血脂水平较高脂组明显降低(均P＜0.05),高密度脂蛋白较高脂组明显升高(P＜0.05);空腹血糖、空腹胰岛素及胰岛素抵抗指数较高脂组明显降低(均P＜0.05),骨骼肌中PPAR-α、ACO及EHHADH mRNA表达较高脂组明显上调(均P＜0.05).结论 游泳运动可显著改善ApoE基因敲除小鼠脂代谢紊乱及胰岛素抵抗,其治疗机制可能与上调骨骼肌中PPAR-α,进一步激活其下游靶基因ACO及EHHADH,从而提高脂肪酸氧化代谢能力有关.
目的 觀察運動對胰島素牴抗小鼠骨骼肌過氧化物酶體增殖物激活受體-α(PPAR-α)及靶基因脂酰輔酶A氧化酶(ACO)、烯酰輔酶A水解酶/L-3羥-CoA脫氫酶(EHHADH)mRNA錶達的影響,併初步探討運動改善脂代謝的可能機製.方法 將20隻雄性ApoE基因敲除小鼠隨機分為高脂組及運動組,高脂組小鼠給予高脂飲食餵養,運動組小鼠在高脂飲食基礎上增加遊泳訓練,另選取C57BL/6J小鼠作為對照組.經榦預12週後,測定各組小鼠空腹血脂、血糖及胰島素濃度,併計算胰島素牴抗指數;同時取各組小鼠腓腸肌進行電鏡超微結構觀察,採用RT-PCR方法檢測各組小鼠骨骼肌中PPAR-α、ACO及EHHADH mRNA水平.結果 通過電鏡觀察髮現,高脂組可見肌細胞線粒體水腫、肌膜下水腫、肌原纖維間水腫,伴有肌溶解竈,該組小鼠血脂(總膽固醇、低密度脂蛋白、遊離脂肪痠)、空腹血糖、空腹胰島素及胰島素牴抗指數均較對照組明顯升高(均P ＜0.05),骨骼肌中PPAR-α、ACO及EHHADH mRNA錶達均較對照組明顯下調(均P＜0.05).運動組小鼠經遊泳榦預後,髮現其肌細胞肌原纖維間有輕度水腫,肌纖維排列整齊,未見肌溶解及肌膜下水腫;血脂水平較高脂組明顯降低(均P＜0.05),高密度脂蛋白較高脂組明顯升高(P＜0.05);空腹血糖、空腹胰島素及胰島素牴抗指數較高脂組明顯降低(均P＜0.05),骨骼肌中PPAR-α、ACO及EHHADH mRNA錶達較高脂組明顯上調(均P＜0.05).結論 遊泳運動可顯著改善ApoE基因敲除小鼠脂代謝紊亂及胰島素牴抗,其治療機製可能與上調骨骼肌中PPAR-α,進一步激活其下遊靶基因ACO及EHHADH,從而提高脂肪痠氧化代謝能力有關.
목적 관찰운동대이도소저항소서골격기과양화물매체증식물격활수체-α(PPAR-α)급파기인지선보매A양화매(ACO)、희선보매A수해매/L-3간-CoA탈경매(EHHADH)mRNA표체적영향,병초보탐토운동개선지대사적가능궤제.방법 장20지웅성ApoE기인고제소서수궤분위고지조급운동조,고지조소서급여고지음식위양,운동조소서재고지음식기출상증가유영훈련,령선취C57BL/6J소서작위대조조.경간예12주후,측정각조소서공복혈지、혈당급이도소농도,병계산이도소저항지수;동시취각조소서비장기진행전경초미결구관찰,채용RT-PCR방법검측각조소서골격기중PPAR-α、ACO급EHHADH mRNA수평.결과 통과전경관찰발현,고지조가견기세포선립체수종、기막하수종、기원섬유간수종,반유기용해조,해조소서혈지(총담고순、저밀도지단백、유리지방산)、공복혈당、공복이도소급이도소저항지수균교대조조명현승고(균P ＜0.05),골격기중PPAR-α、ACO급EHHADH mRNA표체균교대조조명현하조(균P＜0.05).운동조소서경유영간예후,발현기기세포기원섬유간유경도수종,기섬유배렬정제,미견기용해급기막하수종;혈지수평교고지조명현강저(균P＜0.05),고밀도지단백교고지조명현승고(P＜0.05);공복혈당、공복이도소급이도소저항지수교고지조명현강저(균P＜0.05),골격기중PPAR-α、ACO급EHHADH mRNA표체교고지조명현상조(균P＜0.05).결론 유영운동가현저개선ApoE기인고제소서지대사문란급이도소저항,기치료궤제가능여상조골격기중PPAR-α,진일보격활기하유파기인ACO급EHHADH,종이제고지방산양화대사능력유관.
Objective To observe the influence of exercises on the mRNA expression of peroxisome proliferator-activated receptor (PPAR-α) and its target genes of acyl-CoA oxidase (ACO),Enoyl-CoA-hydratase and 3-hydroxyacyl-CoA-dehydrogenase (EHHADH) in the skeletal muscles in insulin-resistance mice to develop a way to improve the lipid metabolism.Methods Twenty male ApoE knockout mice were randomly divided into two groups,the high-fat diet group (group HFD) and the exercise training group (group Ex).The HFD group were fed with highfat diet,while the Ex group were fed in the same way,with additionally swimming training.And ten healthy male C57BL/6j mice were chosen as the control group(group ND).After 12 weeks of intervention,the serum lipid,blood glucose and insulin levels were determined,and homeostasis model assessment insulin resistance index (Homa-IRI) was calculated.The bilateral gastrocnemiuses were cut to be observed under a transmission electron microscope,and the mRNA expression of PPAR-α,ACO and EHHADH in skeletal muscle were measured using reverse transcription polymerase chain reaction(RT-PCR).Results The transmission electron microscope showed that the sarcolemma edema,mitochondrial swelling,as well as focal myocytolysis and edema within myofibrils were observed in the HFD group.The total cholesterol,low-density lipoprotein cholesterol,free fatty acid,fasting glucose,insulin and HomaIRI of the HFD group were significantly higher than the control group (P ＜ 0.05),while the mRNA expression of PPAR-α,ACO and EHHADH was significantly deceased than the latter(P ＜ 0.05).After swimming,the abovementioned pathological changes disappeared.The serum lipid of the Ex group were significantly lower (P ＜ 0.05),while HDL was significantly higher (P ＜ 0.05).And fasting insulin,glucose and HOMA-IR of the Ex group were significantly lower (P ＜ 0.05),while the mRNA expression of the above in the Ex group were significantly increased (P ＜0.05).Conclusion Swimming training could improve insulin resistance and metabolic disorder of lipid of ApoE knockout mice.The possible mechanisms may be through up-regulating the expression of PPAR-α,which in turn stimulates the expression of ACO and EHHADH mRNA to strengthen fatty acid β-oxidation.
