中华物理医学与康复杂志
中華物理醫學與康複雜誌
중화물리의학여강복잡지
CHINESE JOURNAL OF PHYSICAL MEDICINE AND REHABILITATION
2014年
12期
907-912
,共6页
吴银侠%袁小燕%陈新胜%王淑媛%黄承芳
吳銀俠%袁小燕%陳新勝%王淑媛%黃承芳
오은협%원소연%진신성%왕숙원%황승방
参芎葡萄糖注射液%脊髓损伤%AQP-4%Tarlov评分
參芎葡萄糖註射液%脊髓損傷%AQP-4%Tarlov評分
삼궁포도당주사액%척수손상%AQP-4%Tarlov평분
Shenxiong glucose injection%Spinal cord injury%AQP-4%Tarlov score
目的 观察参芎葡萄糖注射液对大鼠脊髓损伤(ScI)后神经功能恢复和水通道蛋白-4(AQP-4)的影响,探讨参芎葡萄糖注射液治疗急性SCI的可能机制.方法 选取正常成年SD大鼠90只,按照随机数字表法将其分为假手术组、SCI组、药物组,每组30只.SCI组和药物组采用改良Allen's打击法建立大鼠SCI模型,假手术组只行椎板切除术,不打击脊髓,药物组术后经腹腔注射参芎葡萄糖30 ml/kg,每日1次,假手术组和SCI组术后给予等量生理盐水.术后ld、3d、7d、14d、21d,分别取6只大鼠进行Tarlov评分,观察大鼠的神经功能恢复情况.采用苏木精-伊红(HE)染色观察大鼠SCI后病理改变,应用免疫荧光染色及免疫印迹法观察SCI后不同时段AQP-4的表达情况.结果 假手术组大鼠术后各时间点Tarlov评分变化不明显(P＞0.05),SCI组和药物组术后Tarlov评分明显下降.随着时间延长,SCI组和药物组大鼠后肢神经功能均有不同程度的恢复,且药物组术后3d、7d、14d、21 dTarlov评分较SCI组显著改善(P＜0.05).术后21d,药物组大鼠病理改变较轻,残留神经元较SCI组多,尚可见核仁.与假手术组同时间点比较,SCI组、药物组各时间点AQP-4表达水平均较高,差异有统计学意义(P＜0.05),与SCI组同时间点比较,药物组各时间点AQP-4表达水平较低,差异有统计学意义(P＜0.05).大鼠Tarlov评分与AQP-4蛋白表达水平在术后3d(r=-0.523,P=0.003)、7d(r=-0.437,P=0.016)、14d(r=-0.417,P=0.022)、21 d(r=-0.377,P=0.040)呈负相关.结论 参芎葡萄糖注射液能有效促进SCI后大鼠的运动功能恢复,其机制可能是通过抑制SCI区AQP-4表达,阻止脊髓水肿发展,进而减轻继发性SCI症状.
目的 觀察參芎葡萄糖註射液對大鼠脊髓損傷(ScI)後神經功能恢複和水通道蛋白-4(AQP-4)的影響,探討參芎葡萄糖註射液治療急性SCI的可能機製.方法 選取正常成年SD大鼠90隻,按照隨機數字錶法將其分為假手術組、SCI組、藥物組,每組30隻.SCI組和藥物組採用改良Allen's打擊法建立大鼠SCI模型,假手術組隻行椎闆切除術,不打擊脊髓,藥物組術後經腹腔註射參芎葡萄糖30 ml/kg,每日1次,假手術組和SCI組術後給予等量生理鹽水.術後ld、3d、7d、14d、21d,分彆取6隻大鼠進行Tarlov評分,觀察大鼠的神經功能恢複情況.採用囌木精-伊紅(HE)染色觀察大鼠SCI後病理改變,應用免疫熒光染色及免疫印跡法觀察SCI後不同時段AQP-4的錶達情況.結果 假手術組大鼠術後各時間點Tarlov評分變化不明顯(P＞0.05),SCI組和藥物組術後Tarlov評分明顯下降.隨著時間延長,SCI組和藥物組大鼠後肢神經功能均有不同程度的恢複,且藥物組術後3d、7d、14d、21 dTarlov評分較SCI組顯著改善(P＜0.05).術後21d,藥物組大鼠病理改變較輕,殘留神經元較SCI組多,尚可見覈仁.與假手術組同時間點比較,SCI組、藥物組各時間點AQP-4錶達水平均較高,差異有統計學意義(P＜0.05),與SCI組同時間點比較,藥物組各時間點AQP-4錶達水平較低,差異有統計學意義(P＜0.05).大鼠Tarlov評分與AQP-4蛋白錶達水平在術後3d(r=-0.523,P=0.003)、7d(r=-0.437,P=0.016)、14d(r=-0.417,P=0.022)、21 d(r=-0.377,P=0.040)呈負相關.結論 參芎葡萄糖註射液能有效促進SCI後大鼠的運動功能恢複,其機製可能是通過抑製SCI區AQP-4錶達,阻止脊髓水腫髮展,進而減輕繼髮性SCI癥狀.
목적 관찰삼궁포도당주사액대대서척수손상(ScI)후신경공능회복화수통도단백-4(AQP-4)적영향,탐토삼궁포도당주사액치료급성SCI적가능궤제.방법 선취정상성년SD대서90지,안조수궤수자표법장기분위가수술조、SCI조、약물조,매조30지.SCI조화약물조채용개량Allen's타격법건립대서SCI모형,가수술조지행추판절제술,불타격척수,약물조술후경복강주사삼궁포도당30 ml/kg,매일1차,가수술조화SCI조술후급여등량생리염수.술후ld、3d、7d、14d、21d,분별취6지대서진행Tarlov평분,관찰대서적신경공능회복정황.채용소목정-이홍(HE)염색관찰대서SCI후병리개변,응용면역형광염색급면역인적법관찰SCI후불동시단AQP-4적표체정황.결과 가수술조대서술후각시간점Tarlov평분변화불명현(P＞0.05),SCI조화약물조술후Tarlov평분명현하강.수착시간연장,SCI조화약물조대서후지신경공능균유불동정도적회복,차약물조술후3d、7d、14d、21 dTarlov평분교SCI조현저개선(P＜0.05).술후21d,약물조대서병리개변교경,잔류신경원교SCI조다,상가견핵인.여가수술조동시간점비교,SCI조、약물조각시간점AQP-4표체수평균교고,차이유통계학의의(P＜0.05),여SCI조동시간점비교,약물조각시간점AQP-4표체수평교저,차이유통계학의의(P＜0.05).대서Tarlov평분여AQP-4단백표체수평재술후3d(r=-0.523,P=0.003)、7d(r=-0.437,P=0.016)、14d(r=-0.417,P=0.022)、21 d(r=-0.377,P=0.040)정부상관.결론 삼궁포도당주사액능유효촉진SCI후대서적운동공능회복,기궤제가능시통과억제SCI구AQP-4표체,조지척수수종발전,진이감경계발성SCI증상.
Objective To explore the action mechanism of shenxiong glucose injection in treatment of acute spinal cord injury(SCI) through observing its effects on the recovery of motor function and the expression of aquaporin-4(AQP-4) in SCI rats.Methods Totally 90 healthy,aduh,Sprague-Dawley rats were randomly divided into a sham operation group (n=30),aSCI group (n=30) and a drug group (n=30).The SCI rat models in both the SCI group and the drup group were established aecording to the modified Allen's method,while the sham operation group was only given laminectomy.After the operation,the drug group was given intraperitoneal shenxiong glucose injection of 30 rnl/kg a day,while the other two groups were injected in the same way with normal saline.The neural function recovery,the pathological changes after SCI and the expressions of AQP-4 were observed 1,3,7,14 and 21 d after the operation using the Tarlov score,the hematoxylin and eosin staining,as well as immunofluorescence techniques and Western blotting.And the correlation of Tarlov scores with AQP-4 expressions was analyzed.Results No significant changes in Tarlov scores were observed in the sham operation group (P ＞ 0.05),while in the SCI group and the drug group,postoperative Tarlov scores decreased significantly.The hindlimb nerve function recovered to some degree with time in the SCI group and drug group.At 3,7,14 and 21 days after the operation,the Tarlov scores in the drug group were significantly higher than the SCI group (P ＜ 0.05).The drug group showed less severe pathological changes,with more residual neurons still visible of nucleoli than the SCI group 21 days after the operation.Compared with the sham operation group,the expression levels of AQP-4 were significantly higher in the SCI group and drug group at all the time points (P ＜ 0.05).However,the expression levels of AQP-4 in the drug group were significantly lower than the SCI group accordingly (P ＜ 0.05).The Tarlov scores were found to be significantly and negatively related to the AQP-4 protein expression levels 3 days(r =-0.523,P =0.003),7 days(r =-0.437,P=0.016),14 days(r=-0.417,P=0.022) and 21 days(r=-0.377,P=0.040)after the operation.Conclusion Injecting shenxiong with glucose can effectively promote the recovery of motor function after SCI,at least in rats.And its mechanism may be that the development of spinal cord edema is prevented and the secondary spinal cord injury alleviated by restraining the expressions of AQ P-4 in the injured areas.
