中华围产医学杂志
中華圍產醫學雜誌
중화위산의학잡지
CHINESE JOURNAL OF PERINATAL MEDICINE
2015年
1期
24-29
,共6页
胡小娅%应燕芬%梁忠杰%林锦%吴海山%林振浪%陈尚勤
鬍小婭%應燕芬%樑忠傑%林錦%吳海山%林振浪%陳尚勤
호소아%응연분%량충걸%림금%오해산%림진랑%진상근
肺%生长和发育%图像处理,计算机辅助%二乙基己基邻苯二甲酸%大鼠
肺%生長和髮育%圖像處理,計算機輔助%二乙基己基鄰苯二甲痠%大鼠
폐%생장화발육%도상처리,계산궤보조%이을기기기린분이갑산%대서
Lung%Growth and development%Image Processing,computer-assisted%Diethylhexyl phthalate%Rats
目的 探讨邻苯二甲酸二乙基己基酯[di-(2-ethylhexyl)phthalate,DEHP]暴露对新生鼠肺形态发育的影响. 方法 无特定病原体(specific pathogen free,SPF)级Sprague-Dawley新生大鼠105只,出生体重5.0～8.0g,共2批.第1批60只大鼠作为对照组,生后第1天起,每日腹腔注射玉米油0.2 ml.第2批45只大鼠作为暴露组,随机分为低、中和高3种剂量亚组,每组1 5只,生后第1～13天,每日腹腔注射溶有DEHP的玉米油0.2 ml,对应的DEHP剂量分别为10、100和750 mg/ (kg·d).对照组分别于生后1、4、7和14d,暴露组于生后第14天,随机取仔鼠6只,称重后腹腔注射戊巴比妥钠35 mg/kg,取右肺中叶近胸膜处肺组织,固定包埋.光镜下观察肺组织病理改变.采用数字图像分析技术分析肺间质比例,测定肺骨架长度并计算肺骨架长度比值.采用单因素方差分析、LSD和Dunnet T3法进行统计学分析. 结果 对照组大鼠肺间质比例生后第1、4、7和14天逐渐下降,分别为(31.97±5.03)％、(30.05±3.57)％、(25.33±1.83)％和(22.01±2.19)％.其中生后第4天分别与第7天和第14天比较,以及第7天与第14天比较,差异均有统计学意义(P＜ 0.05或P＜0.01).生后第14天时,中、高剂量亚组肺间质比例[分别为(24.11±2.78)％和(26.53±3.42)％]均高于对照组(P＜0.05或P＜0.01).对照组肺骨架长度比值生后第1、4、7和14天逐渐增大,分别为0.047 8±0.003 7、0.050 0±0.002 9、0.071 2±0.003 0和0.084 4±0.004 3.生后第4天分别与第7天和第14天比较,以及7和14d比较,差异均有统计学意义(P＜ 0.05或P＜ 0.01).生后14d时,低剂量亚组肺骨架长度比值与中、高剂量亚组比较,差异有统计学意义(分别为0.082 9±0.001 8、0.077 2±0.002 0和0.071 3±0.003 7,P＜ 0.05或P＜ 0.01). 结论 中、高剂量DEHP暴露后,新生大鼠肺间质和肺泡发育受到抑制,暴露量越大,抑制作用越明显.
目的 探討鄰苯二甲痠二乙基己基酯[di-(2-ethylhexyl)phthalate,DEHP]暴露對新生鼠肺形態髮育的影響. 方法 無特定病原體(specific pathogen free,SPF)級Sprague-Dawley新生大鼠105隻,齣生體重5.0～8.0g,共2批.第1批60隻大鼠作為對照組,生後第1天起,每日腹腔註射玉米油0.2 ml.第2批45隻大鼠作為暴露組,隨機分為低、中和高3種劑量亞組,每組1 5隻,生後第1～13天,每日腹腔註射溶有DEHP的玉米油0.2 ml,對應的DEHP劑量分彆為10、100和750 mg/ (kg·d).對照組分彆于生後1、4、7和14d,暴露組于生後第14天,隨機取仔鼠6隻,稱重後腹腔註射戊巴比妥鈉35 mg/kg,取右肺中葉近胸膜處肺組織,固定包埋.光鏡下觀察肺組織病理改變.採用數字圖像分析技術分析肺間質比例,測定肺骨架長度併計算肺骨架長度比值.採用單因素方差分析、LSD和Dunnet T3法進行統計學分析. 結果 對照組大鼠肺間質比例生後第1、4、7和14天逐漸下降,分彆為(31.97±5.03)％、(30.05±3.57)％、(25.33±1.83)％和(22.01±2.19)％.其中生後第4天分彆與第7天和第14天比較,以及第7天與第14天比較,差異均有統計學意義(P＜ 0.05或P＜0.01).生後第14天時,中、高劑量亞組肺間質比例[分彆為(24.11±2.78)％和(26.53±3.42)％]均高于對照組(P＜0.05或P＜0.01).對照組肺骨架長度比值生後第1、4、7和14天逐漸增大,分彆為0.047 8±0.003 7、0.050 0±0.002 9、0.071 2±0.003 0和0.084 4±0.004 3.生後第4天分彆與第7天和第14天比較,以及7和14d比較,差異均有統計學意義(P＜ 0.05或P＜ 0.01).生後14d時,低劑量亞組肺骨架長度比值與中、高劑量亞組比較,差異有統計學意義(分彆為0.082 9±0.001 8、0.077 2±0.002 0和0.071 3±0.003 7,P＜ 0.05或P＜ 0.01). 結論 中、高劑量DEHP暴露後,新生大鼠肺間質和肺泡髮育受到抑製,暴露量越大,抑製作用越明顯.
목적 탐토린분이갑산이을기기기지[di-(2-ethylhexyl)phthalate,DEHP]폭로대신생서폐형태발육적영향. 방법 무특정병원체(specific pathogen free,SPF)급Sprague-Dawley신생대서105지,출생체중5.0～8.0g,공2비.제1비60지대서작위대조조,생후제1천기,매일복강주사옥미유0.2 ml.제2비45지대서작위폭로조,수궤분위저、중화고3충제량아조,매조1 5지,생후제1～13천,매일복강주사용유DEHP적옥미유0.2 ml,대응적DEHP제량분별위10、100화750 mg/ (kg·d).대조조분별우생후1、4、7화14d,폭로조우생후제14천,수궤취자서6지,칭중후복강주사무파비타납35 mg/kg,취우폐중협근흉막처폐조직,고정포매.광경하관찰폐조직병리개변.채용수자도상분석기술분석폐간질비례,측정폐골가장도병계산폐골가장도비치.채용단인소방차분석、LSD화Dunnet T3법진행통계학분석. 결과 대조조대서폐간질비례생후제1、4、7화14천축점하강,분별위(31.97±5.03)％、(30.05±3.57)％、(25.33±1.83)％화(22.01±2.19)％.기중생후제4천분별여제7천화제14천비교,이급제7천여제14천비교,차이균유통계학의의(P＜ 0.05혹P＜0.01).생후제14천시,중、고제량아조폐간질비례[분별위(24.11±2.78)％화(26.53±3.42)％]균고우대조조(P＜0.05혹P＜0.01).대조조폐골가장도비치생후제1、4、7화14천축점증대,분별위0.047 8±0.003 7、0.050 0±0.002 9、0.071 2±0.003 0화0.084 4±0.004 3.생후제4천분별여제7천화제14천비교,이급7화14d비교,차이균유통계학의의(P＜ 0.05혹P＜ 0.01).생후14d시,저제량아조폐골가장도비치여중、고제량아조비교,차이유통계학의의(분별위0.082 9±0.001 8、0.077 2±0.002 0화0.071 3±0.003 7,P＜ 0.05혹P＜ 0.01). 결론 중、고제량DEHP폭로후,신생대서폐간질화폐포발육수도억제,폭로량월대,억제작용월명현.
Objective To investigate the effect of di-(2-ethylhexyl) phthalate (DEHP) on the postnatal lung development in newborn rats.Methods A total of 60 newborn Sprague-Dawley rats (weighing 5.0-8.0 g) in five age groups were studied in the first experiment.The rats were divided based on the different postnatal ages:postnatal day (PND)I,PND4,PND7 and PND14.A total of 45 newborn Sprague-Dawley rats (weighing 5.0-8.0 g) were randomly divided into three groups according to the dosage of DEHP administered in the second experiment.The newborn rats were administered DEHP through intraperitoneal injection at 10 (low-dose subgroup),100 (medium-dose subgroup) or 750 (high-dose subgroup) mg/kg daily from PND1 to PND13.The rats were sacrificed on PND14.Pups were sacrificed with lethal dose injection of pentobarbital sodium.The lung was removed.The right middle lobes were used for analysis.The tissue was processed for histology and lung sections were stained with HE for light microscopic (LM) morphometric measurement.The analysis was performed by means of a digital image analysis system,including pulmonary interstitial area ratio (IAR) and total length density of all segments.One-way ANOVA,LSD and Dunnet T3 methods were used for statistical analysis.Results In the normal controls,IAR decreased significantly by (31.97±5.03) ％,(30.05±3.57)％,(25.33± 1.83)％ and(22.01 ±2.19)％,respectively,from PND1 to PND14 (P＜0.05 or P＜0.01).IAR in medium-and high-dose subgroups increased significantly by (24.11 ±2.78)％ and (26.53± 3.42)％,respectively on PND 14.The total length density of all segments in unit area lung volume increased significantly by 0.047 8±0.003 7,0.050 0±0.002 9,0.071 2±0.003 0 and 0.084 4±0.004 3,respectively from PND1 to PND14 (P＜0.01).In the DEHP treated animals,when compared with the control group,IAR was significantly higher on PND14 (P＜0.05 or P＜0.01),while the total length density of all segments in unit area lung volume was significantly decreased (P＜0.05 or P＜0.01).Length density in medium-and high-dose subgroups were higher than that of low-dose subgroup by 0.082 9±0.001 8,0.077 2±0.002 0 and 0.071 3±0.003 7,respectively on PND14 (P＜0.05 or P＜0.01).Conclusions Medium-and high-dose DEHP affect the postnatal lung development in rats in a dose-dependent mode.
