中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2015年
2期
377-380
,共4页
李杰%刘涛%杜振宁%蔡腾%张丰泉
李傑%劉濤%杜振寧%蔡騰%張豐泉
리걸%류도%두진저%채등%장봉천
双氢青蒿素%骨肉瘤%迁移%尿激酶型纤溶酶原激活物%色素上皮衍生因子
雙氫青蒿素%骨肉瘤%遷移%尿激酶型纖溶酶原激活物%色素上皮衍生因子
쌍경청호소%골육류%천이%뇨격매형섬용매원격활물%색소상피연생인자
Dihydroartemisinin%Osteosarcoma%Migration%Urokinase-type plasminogen activator%Pigment epithelium-derived factor
目的 观察双氢青蒿素(DHA)对人骨肉瘤细胞活性、转移能力以及细胞中尿激酶型纤溶酶原激活物(uPA)和色素上皮衍生因子(PEDF)表达的影响.方法 将不同浓度的DHA(5、10、20、40、80 μmol/L)作用于体外培养的正常人成骨细胞和人骨肉瘤细胞TE85 12、24、48 h后,噻唑蓝(MTT)法测定DHA对细胞活性的影响,反转录-聚合酶链反应(RT-PCR)法检测细胞中uPA和PEDF mRNA水平的表达,Westen blot法检测uPA和PEDF蛋白水平的表达.结果 DHA对人成骨细胞活性无明显影响(P＞0.05).DHA能够明显抑制人骨肉瘤细胞TE85的活性(P＜0.05).不同作用时间下DHA对人骨肉瘤细胞TE85的半数抑制浓度(IC50)分别为(12 h)=(41.66±0.53) μmol/L、IC50(24 h)=(32.28 ±0.27)μmol/L和IC50 (48 h)=(22.53±1.26) μmol/L.DHA能够明显减少人骨肉瘤细胞的迁移和细胞中uPA的表达水平,同时细胞中PEDF表达升高(P＜0.05).结论 DHA对正常人体骨组织无害,能够有效抑制骨肉瘤细胞生长和转移.
目的 觀察雙氫青蒿素(DHA)對人骨肉瘤細胞活性、轉移能力以及細胞中尿激酶型纖溶酶原激活物(uPA)和色素上皮衍生因子(PEDF)錶達的影響.方法 將不同濃度的DHA(5、10、20、40、80 μmol/L)作用于體外培養的正常人成骨細胞和人骨肉瘤細胞TE85 12、24、48 h後,噻唑藍(MTT)法測定DHA對細胞活性的影響,反轉錄-聚閤酶鏈反應(RT-PCR)法檢測細胞中uPA和PEDF mRNA水平的錶達,Westen blot法檢測uPA和PEDF蛋白水平的錶達.結果 DHA對人成骨細胞活性無明顯影響(P＞0.05).DHA能夠明顯抑製人骨肉瘤細胞TE85的活性(P＜0.05).不同作用時間下DHA對人骨肉瘤細胞TE85的半數抑製濃度(IC50)分彆為(12 h)=(41.66±0.53) μmol/L、IC50(24 h)=(32.28 ±0.27)μmol/L和IC50 (48 h)=(22.53±1.26) μmol/L.DHA能夠明顯減少人骨肉瘤細胞的遷移和細胞中uPA的錶達水平,同時細胞中PEDF錶達升高(P＜0.05).結論 DHA對正常人體骨組織無害,能夠有效抑製骨肉瘤細胞生長和轉移.
목적 관찰쌍경청호소(DHA)대인골육류세포활성、전이능력이급세포중뇨격매형섬용매원격활물(uPA)화색소상피연생인자(PEDF)표체적영향.방법 장불동농도적DHA(5、10、20、40、80 μmol/L)작용우체외배양적정상인성골세포화인골육류세포TE85 12、24、48 h후,새서람(MTT)법측정DHA대세포활성적영향,반전록-취합매련반응(RT-PCR)법검측세포중uPA화PEDF mRNA수평적표체,Westen blot법검측uPA화PEDF단백수평적표체.결과 DHA대인성골세포활성무명현영향(P＞0.05).DHA능구명현억제인골육류세포TE85적활성(P＜0.05).불동작용시간하DHA대인골육류세포TE85적반수억제농도(IC50)분별위(12 h)=(41.66±0.53) μmol/L、IC50(24 h)=(32.28 ±0.27)μmol/L화IC50 (48 h)=(22.53±1.26) μmol/L.DHA능구명현감소인골육류세포적천이화세포중uPA적표체수평,동시세포중PEDF표체승고(P＜0.05).결론 DHA대정상인체골조직무해,능구유효억제골육류세포생장화전이.
Objective To investigate the effects of dihydroartemisinin (DHA) on the viability,metastasis and the expression of urokinase-type plasminogen activator (uPA) and pigment epithelium-derived factor (PEDF) in osteosarcoma TE85 cells.Methods The normal human osteoblast cells and osteosarcoma TE85 cells were treated with 5,10,20,40,80 μmol/L DHA for 12,24 and 48 h.After treatment,methyl thiazol tetrazolium (MTT) assay was used to measure the effects of DHA on the viability of normal human osteoblast cells and osteosarcoma TE85 cells.Reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blotting were used to detect the expression of uPA and PEDF in osteosarcoma TE85 cells.Results There was no significant effect of DHA on the viability of normal human osteoblast cells.The viability and the uPA expression of osteosarcoma TE85 cells were significantly inhibited by DHA.However,the PEDF expression was up-regulated by DHA.The 50％ inhibitory dose (IC50) (12 h),IC50(24 h) and IC50(48 h) were respectively (41.66 ±0.53),(32.28 ±0.27) and (22.53 ± 1.26) μmol/L respectively.Conclusion DHA could inhibit the viability and metastasis of osteosarcoma TE85 cells,and had no adverse effects on bones of normal human body.
