CAJ | 학술논문

目的观察低强度跑台运动对大鼠外周血内皮祖细胞(EPCs)数量及内皮型一氧化氮合酶(eNOS)等相关细胞因子的影响并探讨其机制.方法 8周龄SD大鼠24只,随机分为运动组与非运动组,运动组给予低强度跑台运动训练2个月,非运动组同等条件下饲养2个月.2个月后经心脏穿刺抽血,流式细胞术检测CD34+血管内皮生长因子受体(VEGFR)+ EPCs比例,酶联免疫吸附试验(ELISA)法检测血浆中eNOS、血管内皮生长因子(VEGF)及碱性成纤维细胞生长因子(bFGF)等细胞因子的浓度.结果流式细胞术显示运动组外周血EPCs数量(0.612 ±0.018)％增加,与非运动组(0.292±0.027)％比较差异有统计学意义(P＜0.01).ELISA结果显示,eNOS运动组(29.72士0.52)U/L,非运动组(22.85±1.75) U/L(P ＜0.01);VEGF运动组(17.05±0.34) U/L,非运动组(5.61±0.45) U/L(P＜0.01);bFGF运动组(66.98±1.80) U/L,非运动组(51.21±1.90) U/L(P ＜0.01).以上结果显示运动组各细胞因子浓度升高,与非运动组比较差异有统计学意义.结论低强度运动可以刺激大鼠体内细胞因子增多并增加外周血中EPCs的数量,而细胞因子的增多可能是动员SD大鼠EPCs人血和迁移的分子机制.
목적 관찰저강도포태운동대대서외주혈내피조세포(EPCs)수량급내피형일양화담합매(eNOS)등상관세포인자적영향병탐토기궤제.방법 8주령SD대서24지,수궤분위운동조여비운동조,운동조급여저강도포태운동훈련2개월,비운동조동등조건하사양2개월.2개월후경심장천자추혈,류식세포술검측CD34+혈관내피생장인자수체(VEGFR)+ EPCs비례,매련면역흡부시험(ELISA)법검측혈장중eNOS、혈관내피생장인자(VEGF)급감성성섬유세포생장인자(bFGF)등세포인자적농도.결과 류식세포술현시운동조외주혈EPCs수량(0.612 ±0.018)％증가,여비운동조(0.292±0.027)％비교차이유통계학의의(P＜0.01).ELISA결과현시,eNOS운동조(29.72사0.52)U/L,비운동조(22.85±1.75) U/L(P ＜0.01);VEGF운동조(17.05±0.34) U/L,비운동조(5.61±0.45) U/L(P＜0.01);bFGF운동조(66.98±1.80) U/L,비운동조(51.21±1.90) U/L(P ＜0.01).이상결과현시운동조각세포인자농도승고,여비운동조비교차이유통계학의의.결론 저강도운동가이자격대서체내세포인자증다병증가외주혈중EPCs적수량,이세포인자적증다가능시동원SD대서EPCs인혈화천이적분자궤제.
Objective To observe the impact and explore its mechanism of low intensity treadmill exercise on the number of endothelial progenitor cells and the concentration of cytokines such as endothelial nitric oxide synthase (eNOS) in Sprague-Dawley (SD) rats.Methods Twenty four eight-weeks SD rats were randomly divided into two groups (Exercise group and Non-exercise group).Exercise group was exposed to two-month low intensity treadmill exercise,and Non-exercise group not.Both groups were harvested blood samples via cardiac puncture two months later.CD34 + vascular endothelial growth factor receptor (VEGFR) + double-positive cells (EPCs) were tested with flow cytometry,and cytokines as eNOS,vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) were detected using enzyme linked immunosorbent assay (ELISA).Results The number of EPCs increased in Exercise group (0.612±0.018) ％,compared with the Non-exercise group (0.292 ±0.027) ％ (P＜0.01).The concentration of cytokines in Exercise group were higher than Non-exercise group as follow:eNOS (29.72 ±0.52) U/L vs.(22.85 ± 1.75) U/L (P＜0.01) ; VEGF (17.05 ±0.34) U/L vs.(5.61 ± 0.45) U/L (P＜0.01); bFGF (66.98±1.80) U/L vs.(51.21 ±1.90) U/L (P＜0.01).Conclusion Low intensity treadmill exercise increased the number of EPCs and the concentration of cytokines in SD rats.And the increment of cytokines may be the molecular mechanism to mobilize and migrate the EPCs.