中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2015年
2期
391-393
,共3页
涂华华%陈先祥%程彩涛%冯勇%张林菲%曾天才%蔡庆和
塗華華%陳先祥%程綵濤%馮勇%張林菲%曾天纔%蔡慶和
도화화%진선상%정채도%풍용%장림비%증천재%채경화
甲状腺癌%甲状腺腺瘤%S100钙结合蛋白A13%人成纤维细胞生长因子-1
甲狀腺癌%甲狀腺腺瘤%S100鈣結閤蛋白A13%人成纖維細胞生長因子-1
갑상선암%갑상선선류%S100개결합단백A13%인성섬유세포생장인자-1
Thyroid cancer%Thyroid adenoma%S100 calcium binding protein A13%Fibroblast growth factor-1
目的 检测S100钙结合蛋白A13(S100A13)和人成纤维细胞生长因子-1(FGF-1)在甲状腺癌、甲状腺腺瘤、正常甲状腺组织中的表达,探讨两者与甲状腺癌发生发展的关系.方法 采用免疫组织化学链霉菌抗生物素蛋白-过氧化物酶(SP)方法检测甲状腺癌、甲状腺腺瘤、正常甲状腺组织中两者的表达,比较两者在两种不同组织中的表达.结果 甲状腺癌组织中,S100A13的表达阳性率为93.3％(56/60),在甲状腺腺瘤组织中S100A13的表达阳性率为66.7％ (20/30),在正常甲状腺组织中的表达阳性率为60.0％(12/20),S100A13在甲状腺癌组织的表达阳性率明显高于甲状腺腺瘤及正常甲状腺组织,差异有统计学意义(P＜0.05),但是在甲状腺腺瘤及正常甲状腺组织中其表达差异无统计学意义(P＞0.05);FGF-1在甲状腺癌组织中的表达阳性率为81.7％(49/60),在甲状腺腺瘤组织中FGF-1的表达阳性率为63.3％(19/30),在正常甲状腺组织中的表达阳性率为55.0％(11/20),FGF-1在甲状腺癌组织的表达阳性率明显高于甲状腺腺瘤及正常甲状腺组织,差异有统计学意义(P＜0.05),但是在甲状腺腺瘤及正常甲状腺组织中其表达差异无统计学意义(P＞0.05);S100A13与FGF-1表达阳性与甲状腺癌的淋巴结转移相关(P＜0.05).结论 S100A13与FGF-1与甲状腺癌的发生及发展密切相关.
目的 檢測S100鈣結閤蛋白A13(S100A13)和人成纖維細胞生長因子-1(FGF-1)在甲狀腺癌、甲狀腺腺瘤、正常甲狀腺組織中的錶達,探討兩者與甲狀腺癌髮生髮展的關繫.方法 採用免疫組織化學鏈黴菌抗生物素蛋白-過氧化物酶(SP)方法檢測甲狀腺癌、甲狀腺腺瘤、正常甲狀腺組織中兩者的錶達,比較兩者在兩種不同組織中的錶達.結果 甲狀腺癌組織中,S100A13的錶達暘性率為93.3％(56/60),在甲狀腺腺瘤組織中S100A13的錶達暘性率為66.7％ (20/30),在正常甲狀腺組織中的錶達暘性率為60.0％(12/20),S100A13在甲狀腺癌組織的錶達暘性率明顯高于甲狀腺腺瘤及正常甲狀腺組織,差異有統計學意義(P＜0.05),但是在甲狀腺腺瘤及正常甲狀腺組織中其錶達差異無統計學意義(P＞0.05);FGF-1在甲狀腺癌組織中的錶達暘性率為81.7％(49/60),在甲狀腺腺瘤組織中FGF-1的錶達暘性率為63.3％(19/30),在正常甲狀腺組織中的錶達暘性率為55.0％(11/20),FGF-1在甲狀腺癌組織的錶達暘性率明顯高于甲狀腺腺瘤及正常甲狀腺組織,差異有統計學意義(P＜0.05),但是在甲狀腺腺瘤及正常甲狀腺組織中其錶達差異無統計學意義(P＞0.05);S100A13與FGF-1錶達暘性與甲狀腺癌的淋巴結轉移相關(P＜0.05).結論 S100A13與FGF-1與甲狀腺癌的髮生及髮展密切相關.
목적 검측S100개결합단백A13(S100A13)화인성섬유세포생장인자-1(FGF-1)재갑상선암、갑상선선류、정상갑상선조직중적표체,탐토량자여갑상선암발생발전적관계.방법 채용면역조직화학련매균항생물소단백-과양화물매(SP)방법검측갑상선암、갑상선선류、정상갑상선조직중량자적표체,비교량자재량충불동조직중적표체.결과 갑상선암조직중,S100A13적표체양성솔위93.3％(56/60),재갑상선선류조직중S100A13적표체양성솔위66.7％ (20/30),재정상갑상선조직중적표체양성솔위60.0％(12/20),S100A13재갑상선암조직적표체양성솔명현고우갑상선선류급정상갑상선조직,차이유통계학의의(P＜0.05),단시재갑상선선류급정상갑상선조직중기표체차이무통계학의의(P＞0.05);FGF-1재갑상선암조직중적표체양성솔위81.7％(49/60),재갑상선선류조직중FGF-1적표체양성솔위63.3％(19/30),재정상갑상선조직중적표체양성솔위55.0％(11/20),FGF-1재갑상선암조직적표체양성솔명현고우갑상선선류급정상갑상선조직,차이유통계학의의(P＜0.05),단시재갑상선선류급정상갑상선조직중기표체차이무통계학의의(P＞0.05);S100A13여FGF-1표체양성여갑상선암적림파결전이상관(P＜0.05).결론 S100A13여FGF-1여갑상선암적발생급발전밀절상관.
Objective To detect S100 calcium binding protein A13 (S100A13) and fibroblast growth factor-1 (FGF-1) in thyroid cancer,thyroid adenoma and normal thyroid tissue,and to explore their relationship with the development of thyroid cancer.Methods Streptavidin avidin-peroxidase (SP) immunohistochemical method was used to detect the expression of S100A13 and FGF-1 in thyroid cancer,thyroid adenoma and normal thyroid tissues.Results The expression rate of S100A13 in thyroid cancer,thyroid adenoma and normal thyroid tissues was 93.3％ (56/60),66.7％ (20/30) and 60.0％ (12/20) respectively.The S100A13 expression rate in thyroid cancer and thyroid adenoma tissues was significantly higher than in normal thyroid tissue (P ＜ 0.05),but there was no significant difference between thyroid adenoma and normal thyroid tissues (P ＞ 0.05).The expression rate of FGF-1 in thyroid cancer,thyroid adenoma and normal thyroid tissues was 81.7％ (49/60),63.3％ (19/30),and 55.0％ (11/20) respectively.The positive rate of FGF-1 expression in thyroid cancer tissues was significantly higher than that in thyroid adenoma and normal thyroid tissues (P ＜ 0.05),but there was no significant difference between thyroid adenoma and normal thyroid tissues (P＞ 0.05).The positive expression of S100A13 and FGF-1 was associated with lymph node metastasis (P ＜0.05).Conclusion S100A13 and FGF-1 are closely related to the occurrence and development of thyroid cancer.
