中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2015年
2期
419-421
,共3页
李杰%刘涛%杜振宁%蔡腾%张丰泉
李傑%劉濤%杜振寧%蔡騰%張豐泉
리걸%류도%두진저%채등%장봉천
微小RNA-146a%骨肉瘤%脱噬作用
微小RNA-146a%骨肉瘤%脫噬作用
미소RNA-146a%골육류%탈서작용
MicroRNA-146a%Osteosarcoma%Apoptosis
目的 探讨微小RNA(miRNA,miR)-146a在骨肉瘤中表达的意义和miRNA-146a对骨肉瘤的作用机制.方法 实时定量聚合酶链反应(Real-time PCR)检测骨肉瘤标本和癌旁组织中miRNA-146a表达量,并采用Cox回归模型分析其与患者生存期的相关性.质粒转染miRNA-146a进入骨肉瘤细胞TE85中,检测转染后细胞活性、细胞凋亡和细胞中YY1、Fas蛋白的表达.结果 癌旁组织和骨肉瘤组织中miRNA-146a的表达量分别为1.213±0.525和0.734±0.263,差异有统计学意义(P＜0.05).miRNA-146a的表达量与患者的生存时间呈正相关(P＜0.05).转染miRNA-146a24 h后,50、100 nmol/L组人骨肉瘤细胞活性明显降低至(65.161±5.528)％和(40.385±8.442)％(P＜0.05),细胞凋亡率分别明显升高至(38.671±12.415)％和(59.513±9.058)％ (P＜0.05),细胞中YY1蛋白表达量明显降低(分别为0.472±0.014和0.272±0.005,P＜0.05),而Fas蛋白表达量明显升高(0.292±0.010、0.584±0.006,P＜0.05).转染miRNA-146a 48 h后,与对照组比较,50、100 nmol/L组人骨肉瘤细胞活性、凋亡率、YY1表达量和Fas蛋白表达量变化与转染24 h的变化相似.结论 miRNA-146a对骨肉瘤的作用是通过下调YY1的表达,而上调Fas蛋白的表达,抑制骨肉瘤细胞的增殖同时促进骨肉瘤细胞的凋亡.
目的 探討微小RNA(miRNA,miR)-146a在骨肉瘤中錶達的意義和miRNA-146a對骨肉瘤的作用機製.方法 實時定量聚閤酶鏈反應(Real-time PCR)檢測骨肉瘤標本和癌徬組織中miRNA-146a錶達量,併採用Cox迴歸模型分析其與患者生存期的相關性.質粒轉染miRNA-146a進入骨肉瘤細胞TE85中,檢測轉染後細胞活性、細胞凋亡和細胞中YY1、Fas蛋白的錶達.結果 癌徬組織和骨肉瘤組織中miRNA-146a的錶達量分彆為1.213±0.525和0.734±0.263,差異有統計學意義(P＜0.05).miRNA-146a的錶達量與患者的生存時間呈正相關(P＜0.05).轉染miRNA-146a24 h後,50、100 nmol/L組人骨肉瘤細胞活性明顯降低至(65.161±5.528)％和(40.385±8.442)％(P＜0.05),細胞凋亡率分彆明顯升高至(38.671±12.415)％和(59.513±9.058)％ (P＜0.05),細胞中YY1蛋白錶達量明顯降低(分彆為0.472±0.014和0.272±0.005,P＜0.05),而Fas蛋白錶達量明顯升高(0.292±0.010、0.584±0.006,P＜0.05).轉染miRNA-146a 48 h後,與對照組比較,50、100 nmol/L組人骨肉瘤細胞活性、凋亡率、YY1錶達量和Fas蛋白錶達量變化與轉染24 h的變化相似.結論 miRNA-146a對骨肉瘤的作用是通過下調YY1的錶達,而上調Fas蛋白的錶達,抑製骨肉瘤細胞的增殖同時促進骨肉瘤細胞的凋亡.
목적 탐토미소RNA(miRNA,miR)-146a재골육류중표체적의의화miRNA-146a대골육류적작용궤제.방법 실시정량취합매련반응(Real-time PCR)검측골육류표본화암방조직중miRNA-146a표체량,병채용Cox회귀모형분석기여환자생존기적상관성.질립전염miRNA-146a진입골육류세포TE85중,검측전염후세포활성、세포조망화세포중YY1、Fas단백적표체.결과 암방조직화골육류조직중miRNA-146a적표체량분별위1.213±0.525화0.734±0.263,차이유통계학의의(P＜0.05).miRNA-146a적표체량여환자적생존시간정정상관(P＜0.05).전염miRNA-146a24 h후,50、100 nmol/L조인골육류세포활성명현강저지(65.161±5.528)％화(40.385±8.442)％(P＜0.05),세포조망솔분별명현승고지(38.671±12.415)％화(59.513±9.058)％ (P＜0.05),세포중YY1단백표체량명현강저(분별위0.472±0.014화0.272±0.005,P＜0.05),이Fas단백표체량명현승고(0.292±0.010、0.584±0.006,P＜0.05).전염miRNA-146a 48 h후,여대조조비교,50、100 nmol/L조인골육류세포활성、조망솔、YY1표체량화Fas단백표체량변화여전염24 h적변화상사.결론 miRNA-146a대골육류적작용시통과하조YY1적표체,이상조Fas단백적표체,억제골육류세포적증식동시촉진골육류세포적조망.
Objective To study the expressiou and significance of microRNA (miRNA,miR)-146a in osteosarcoma.Methods The expression levels of miRNA-146a in osteosarcoma tissue and normal tissue adjacent to carcinoma were detected by real-time quantitative polymerase chain reaction (Real-time PCR).The correlation between survival and miRNA-146a level was analyzed by Cox' s proportional hazards regression model.The cell viabiliyt,apoptosis and the levels of Yin-Yang 1 (YY1) and Fas were measured after miRNA-146a was transfected into osteosarcoma TE85 cells.Results The expression levels of miRNA-146a in osteosarcoma tissue and normal tissue adjacent to carcinoma were respectively 1.213 ±0.525 and 0.734 ±0.263.There was a positive correlation between survival and miRNA-146a expression level (P ＜0.05).After transfected with 50,100 nmol/L miRNA-146a for 24 h,the cytoactive were respectively (65.161 ±5.528)％ and (40.385 ±8.442)％ (P＜0.05) and the apoptosis rates were respectively (38.671 ± 12.415) ％ and (59.513 ± 9.058) ％ (P ＜0.05).The YY1 expression levels were significantly decreased (0.472 ± 0.014 and 0.272 ± 0.005,P ＜ 0.05),and the Fas expression level were significantly increased (0.292 ± 0.010 and 0.584 ± 0.006,P ＜ 0.05).Compared to control group,the changes of cytoactive,apoptosis rates,YY and Fas expression levels after transfected with 50 and 100 nmol/L miRNA-46a for 48 h were similar to those changes in osteosarcoma cells transfected with miRNA-146a for 24 h.Conclusion miRNA-146a may inhibit the proliferation of osteosarcoma cells,and simultaneously promote the apoptosis of osteosarcoma cells,probably by down-regulating the YY1 expression and up-regulating the Fas expression.
