中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2015年
2期
422-423
,共2页
胰腺癌%裸鼠%原位模型%高转移
胰腺癌%裸鼠%原位模型%高轉移
이선암%라서%원위모형%고전이
Pancreatic cancer%Nude mouse%Orthotopic model%Highly metastatic
目的 研究一种成功率高、转移率高的人胰腺癌原位动物造模方法.方法 将MIAPaCa-2细胞接种到裸鼠的皮下,待其成瘤后,再将皮下瘤块无菌条件下取出,原位种植到30只裸鼠胰腺尾部,于4、6、8周分批处死裸鼠,进行大体及组织学观察和分析.结果 本研究裸鼠胰腺原位成瘤率达100％,苏木素-伊红(HE)染色观察发现4、6、8周裸鼠原位成瘤的胰腺癌细胞形态学上无明显差异,均为低分化胰腺癌细胞.镜下证实原位植瘤后4周裸鼠远处转移率为0％,原位移植瘤后8周远处转移率为100％,包括腹腔淋巴结转移及远处脏器转移,两者差异有统计学意义(P＜0.05).肝转移仅为1例,占10％.结论 裸鼠4周成瘤属于胰腺癌的早期,肿瘤未发生转移;8周成瘤属于胰腺癌晚期,肿瘤发生了淋巴结及远处转移,且利用该种建模方法成功率高、转移率高、肝转移率低.
目的 研究一種成功率高、轉移率高的人胰腺癌原位動物造模方法.方法 將MIAPaCa-2細胞接種到裸鼠的皮下,待其成瘤後,再將皮下瘤塊無菌條件下取齣,原位種植到30隻裸鼠胰腺尾部,于4、6、8週分批處死裸鼠,進行大體及組織學觀察和分析.結果 本研究裸鼠胰腺原位成瘤率達100％,囌木素-伊紅(HE)染色觀察髮現4、6、8週裸鼠原位成瘤的胰腺癌細胞形態學上無明顯差異,均為低分化胰腺癌細胞.鏡下證實原位植瘤後4週裸鼠遠處轉移率為0％,原位移植瘤後8週遠處轉移率為100％,包括腹腔淋巴結轉移及遠處髒器轉移,兩者差異有統計學意義(P＜0.05).肝轉移僅為1例,佔10％.結論 裸鼠4週成瘤屬于胰腺癌的早期,腫瘤未髮生轉移;8週成瘤屬于胰腺癌晚期,腫瘤髮生瞭淋巴結及遠處轉移,且利用該種建模方法成功率高、轉移率高、肝轉移率低.
목적 연구일충성공솔고、전이솔고적인이선암원위동물조모방법.방법 장MIAPaCa-2세포접충도라서적피하,대기성류후,재장피하류괴무균조건하취출,원위충식도30지라서이선미부,우4、6、8주분비처사라서,진행대체급조직학관찰화분석.결과 본연구라서이선원위성류솔체100％,소목소-이홍(HE)염색관찰발현4、6、8주라서원위성류적이선암세포형태학상무명현차이,균위저분화이선암세포.경하증실원위식류후4주라서원처전이솔위0％,원위이식류후8주원처전이솔위100％,포괄복강림파결전이급원처장기전이,량자차이유통계학의의(P＜0.05).간전이부위1례,점10％.결론 라서4주성류속우이선암적조기,종류미발생전이;8주성류속우이선암만기,종류발생료림파결급원처전이,차이용해충건모방법성공솔고、전이솔고、간전이솔저.
Objective To study a method of orthotopic transplantation model of pancreatic cancer with high success rate and high transfer rate.Methods This research used the MIA PaCa-2 cell lines to establish a nude mouse orthotopic transplantation model of pancreatic cancer.The cancer cells were subcutaneously injected into 30 nude mice,after being tumor,removed subcutaneous tumor with sterile conditions,grew into nude mice pancreas tail in situ.Morphological and biological features,and metastasis of the transplanted tumor were studied after 4,6 and 8 weeks.Results In this study the rate of tumor was 100％,and there was no obvious difference from the cells morphology of pancreatic tumor between 4 weeks and 8 weeks with hematoxylin and eosin (HE) staining,there were poorly differentiated pancreatic cancer cells.The distant metastasis rate was 0％ in postoperative 4 weeks by the microscopic observation,distant metastasis in nude mice was 100％ in postoperative 8 weeks,including local lymph node metastasis and distant organ metastasis,there were extremely significant statistical differences (P ＜ 0.05).The hepatic metastasis rate was 10％ only.Conclusion That showed tumor of 4 weeks belonged to pancreatic cancer early in the disease process,and tumor of 8 weeks belonged to the late stages of pancreatic cancer disease process,it is high success rate,high transfer rate and low hepatic metastasis rate of using this method to build the orthotopic transplantation model.
