CAJ | 학술논문

目的观察在大脑中动脉闭塞(MCAO)模型中不同缺血时间对实验结果的影响.方法雄性SD大鼠随机分为缺血60、90和120 min 3组,在再灌注开始时吸入1.0最小肺泡浓度(MAC)七氟烷进行后处理.再灌注24h后用神经功能缺陷评分评价神经功能,氯化三苯基四氮唑(TTC)染色后计算脑梗死容积.结果 60、90和120 min缺血导致的脑梗死容积分别为26.35％、42.65％和50.02％.在90 min组,七氟烷后处理明显改善了神经功能,七氟烷组和对照组神经功能缺陷评分均数分别为1.41和2.22分,并且减小了脑梗死容积,梗死容积百分比分别为30.17％和42.65％,60和120 min组比较差异无统计学意义(P＞0.05).结论选择恰当的缺血时间是MCAO模型动物实验取得良好实验结果的关键.
목적 관찰재대뇌중동맥폐새(MCAO)모형중불동결혈시간대실험결과적영향.방법 웅성SD대서수궤분위결혈60、90화120 min 3조,재재관주개시시흡입1.0최소폐포농도(MAC)칠불완진행후처리.재관주24h후용신경공능결함평분평개신경공능,록화삼분기사담서(TTC)염색후계산뇌경사용적.결과 60、90화120 min결혈도치적뇌경사용적분별위26.35％、42.65％화50.02％.재90 min조,칠불완후처리명현개선료신경공능,칠불완조화대조조신경공능결함평분균수분별위1.41화2.22분,병차감소료뇌경사용적,경사용적백분비분별위30.17％화42.65％,60화120 min조비교차이무통계학의의(P＞0.05).결론 선택흡당적결혈시간시MCAO모형동물실험취득량호실험결과적관건.
Objective To evaluate the effect of different ischemic time on sevoflurane postconditioning in rat middle cerebral artery occlusion model (MCAO).Methods Male SD rats were randomly allocated into 3 groups subjected to MCAO for 60 min,90 min or 120 min separately and then treated with sevoflurane at the beginning of reperfusion.The infarct volume and neurological deficit scores were evaluated at 24 hours.Results The infarct volume of 60,90 and 120 min group is 26.35％,42.65％ and 50.02％.In 90 min group,postconditioning with sevoflurane deceased neurological deficit scores (1.41 vs.2.22) and infarct volume (30.17％ vs.42.65％),while no significant differences were observed in 60 min and 120 min groups.Conclusion Optimal ischemic time is very important in experiment with MCAO.