中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2015年
2期
370-373
,共4页
高肺血流肺动脉高压%幼猪模型%血管内皮祖细胞
高肺血流肺動脈高壓%幼豬模型%血管內皮祖細胞
고폐혈류폐동맥고압%유저모형%혈관내피조세포
High blood-induced pulmonary artery hypertension%Piglet model%Endothelial progenitor cells
目的 观察自体血管内皮祖细胞(EPCs)移植对高肺血流肺动脉高压(PAH)的治疗作用.方法 将30头实验幼猪随机分为3组,每组10头.假手术组(Sham组)仅开胸;对照组(Control组)行左胸后外侧切口,降主动脉与主肺动脉分流术;EPCs治疗组(EPCs组)手术操作同对照组,术后2周给予EPCs 2×10 7个细胞,颈静脉输入.术前与术后30 d,观察3组猪的肺动脉收缩压(PASP)、肺血管阻力(PVR),血清基质金属蛋白酶(MMP)-9、内皮素-1(ET-1)、白细胞介素(IL)-6、IL-8水平变化,以及肺组织p38丝裂原激活蛋白激酶(p38 MAPK)表达活性,肺动脉标本的组织学变化.结果 术后30 d,与Sham组比较,PASP、PVR、MMP-9、ET-1、IL-6、IL-8、p38MAPK在Control组[(6.63±1.20) kPa、(10.44 ±2.53) wood's U、(76.63±10.39) μg/L、(103.66±17.31) μg/L、(43.00 ±7.33) ng/L、(67.39±9.65) ng/L、3.63 ±0.33]和EPCs组[(4.07 ±0.77) kPa、(6.27±0.65) wood's U、(64.11 ±9.73) μg/L、(90.44±13.11) μg/L、(30.44±8.17) ng/L、(53.23±7.37) ng/L、1.73 ±0.37]明显升高(P＜0.01);EPCs组明显低于Control组(P＜0.05);肺组织病理学改变,Control组最为明显,EPCs组轻度改变,Sham组未见明显病理改变.结论 EPCs移植对高肺血流肺动脉高压具有治疗作用,其机制可能为抑制MMP-9、ET-1等致肺组织重构因素的水平,以及抑制肺动脉高压过程中的炎症损伤而发挥作用.
目的 觀察自體血管內皮祖細胞(EPCs)移植對高肺血流肺動脈高壓(PAH)的治療作用.方法 將30頭實驗幼豬隨機分為3組,每組10頭.假手術組(Sham組)僅開胸;對照組(Control組)行左胸後外側切口,降主動脈與主肺動脈分流術;EPCs治療組(EPCs組)手術操作同對照組,術後2週給予EPCs 2×10 7箇細胞,頸靜脈輸入.術前與術後30 d,觀察3組豬的肺動脈收縮壓(PASP)、肺血管阻力(PVR),血清基質金屬蛋白酶(MMP)-9、內皮素-1(ET-1)、白細胞介素(IL)-6、IL-8水平變化,以及肺組織p38絲裂原激活蛋白激酶(p38 MAPK)錶達活性,肺動脈標本的組織學變化.結果 術後30 d,與Sham組比較,PASP、PVR、MMP-9、ET-1、IL-6、IL-8、p38MAPK在Control組[(6.63±1.20) kPa、(10.44 ±2.53) wood's U、(76.63±10.39) μg/L、(103.66±17.31) μg/L、(43.00 ±7.33) ng/L、(67.39±9.65) ng/L、3.63 ±0.33]和EPCs組[(4.07 ±0.77) kPa、(6.27±0.65) wood's U、(64.11 ±9.73) μg/L、(90.44±13.11) μg/L、(30.44±8.17) ng/L、(53.23±7.37) ng/L、1.73 ±0.37]明顯升高(P＜0.01);EPCs組明顯低于Control組(P＜0.05);肺組織病理學改變,Control組最為明顯,EPCs組輕度改變,Sham組未見明顯病理改變.結論 EPCs移植對高肺血流肺動脈高壓具有治療作用,其機製可能為抑製MMP-9、ET-1等緻肺組織重構因素的水平,以及抑製肺動脈高壓過程中的炎癥損傷而髮揮作用.
목적 관찰자체혈관내피조세포(EPCs)이식대고폐혈류폐동맥고압(PAH)적치료작용.방법 장30두실험유저수궤분위3조,매조10두.가수술조(Sham조)부개흉;대조조(Control조)행좌흉후외측절구,강주동맥여주폐동맥분류술;EPCs치료조(EPCs조)수술조작동대조조,술후2주급여EPCs 2×10 7개세포,경정맥수입.술전여술후30 d,관찰3조저적폐동맥수축압(PASP)、폐혈관조력(PVR),혈청기질금속단백매(MMP)-9、내피소-1(ET-1)、백세포개소(IL)-6、IL-8수평변화,이급폐조직p38사렬원격활단백격매(p38 MAPK)표체활성,폐동맥표본적조직학변화.결과 술후30 d,여Sham조비교,PASP、PVR、MMP-9、ET-1、IL-6、IL-8、p38MAPK재Control조[(6.63±1.20) kPa、(10.44 ±2.53) wood's U、(76.63±10.39) μg/L、(103.66±17.31) μg/L、(43.00 ±7.33) ng/L、(67.39±9.65) ng/L、3.63 ±0.33]화EPCs조[(4.07 ±0.77) kPa、(6.27±0.65) wood's U、(64.11 ±9.73) μg/L、(90.44±13.11) μg/L、(30.44±8.17) ng/L、(53.23±7.37) ng/L、1.73 ±0.37]명현승고(P＜0.01);EPCs조명현저우Control조(P＜0.05);폐조직병이학개변,Control조최위명현,EPCs조경도개변,Sham조미견명현병리개변.결론 EPCs이식대고폐혈류폐동맥고압구유치료작용,기궤제가능위억제MMP-9、ET-1등치폐조직중구인소적수평,이급억제폐동맥고압과정중적염증손상이발휘작용.
Objective To stidy the effects of the transplantation of autologous endothelial progenitor cells (EPCs) on high blood flow-induced pulmonary artery hypertension (PAH).Methods Thirty piglets were divided into three groups randomly.The animals in sham group (n =10) underwent thoracotomy only,those in control group (n =10) were subjected to the descending aorta and the main pulmonary artery shunt via left posterolateral thoracotomy,and those in EPCs + shunt group (n =10) were given EPCs (2 × 107) intravenously after descending aorta and the main pulmonary artery shunt procedure for two weeks.All animals are normally raised for one month.Then their pulmonary artery systolic pressure (PASP) and pulmonary vascular resistance (PVR) were measured,the levels of serum matrix metalloproteinase (MMP)-9,endothelin (ET)-1,interleukin (IL)-6,and IL-8 were tested,p38 mitogen-activated protein kinase (p38MAPK) activity in the lung tissue was detected,and pulmonary artery structures were observed.Results As compared with the Sham group,the levels of PASP,PVR,MMP-9,ET-1,IL-6 and IL-8,and activity of p38MAPK were significantly increased in control group [(6.63 ± 1.20) kPa,(10.44±2.53) wood's U,(76.63±10.39) μg/L,(103.66±17.31) μg/L,(43.00± 7.33) ng/L,(67.39 ±9.65) ng/L,3.63 ±0.33] and EPCs + shunt group [(4.07 ±0.77) kPa,(6.27 ± 0.65) wood' s U,(64.11 ± 9.73) μg/L,(90.44 ± 13.11) μg/L,(30.44 ± 8.17) ng/L,(53.23 ± 7.37) ng/L,1.73 ± 0.37] (P ＜ 0.01),30 days postoperatively.These values were remarkablely decreased in EPCs + shunt group as compared with those in control group (P ＜ 0.05).There were most obvious lung histologic changes in control group,mild changes in EPCs + shunt group,and no obvious pathological changes in Sham group.Conclusion Transplantation of autologous EPCs attenuates dynamic pulmonary hypertension,which may be associated with the decreased levels of MMP-9 and ET-1 which cause the lung tissue reconstruction,and inhibition of the inflammatory injury which plays a leader role in the process of PAH.
