姜酚对家兔胸主动脉平滑肌的舒张作用及其机制
강분대가토흉주동맥평활기적서장작용급기궤제
Vasodilative effect and the underlying mechanism of gingerol on isolated thoracic aorta ring of rabbits
  • 万方数据
    中华实验外科杂志 중화실험외과잡지
    CHINESE JOURNAL OF EXPERIMENTAL SURGERY
    2015年 2期 265-268 ,共4页

    金燕%赵文超%王志举

    금연%조문초%왕지거

    姜酚%胸主动脉%内皮%一氧化氮%前列环素 薑酚%胸主動脈%內皮%一氧化氮%前列環素
    강분%흉주동맥%내피%일양화담%전렬배소
    Gingerol%Thoracic aorta%Endothelium%Nitric oxide%Prostacyclin
    目的 观察姜酚(gingerol)对家兔胸主动脉平滑肌的舒张作用并探讨其作用机制.方法 制备离体内皮完整的和去内皮胸主动脉环肌条,悬挂于恒温浴槽中,并将其与张力-位移换能器相连记录血管环肌条张力的变化,所有标本分为内皮完整组和去内皮组.内皮完整组分为1×10-5 mol/L去甲肾上腺素(NE)+不同浓度gingerol组(1×10-8、1 ×10-7、1 ×10-6、1 ×10-5、1×10-4、1×10-3 mol/L,下同)、20 mmol/L KCl+不同浓度gingerol组、1×10-5 mol/L NE+左旋-硝基精氨酸甲脂(L-NAME)+不同浓度gingerol组、1×10-5 mol/L NE+吲哚美辛+不同浓度gingerol组、20 mmol/LKCl+ L-NAME+不同浓度gingerol组、20 mmol/L KCl+吲哚美辛+不同浓度gingerol组;去内皮组分为1×10-5 mol/L NE+不同浓度gingerol组、20 mmol/L KCl+不同浓度gingerol组.结果 (1)gingerol能抑制1×10-5 mol/L的NE或20 mmol/L的KCl引起的胸主动脉平滑肌收缩并呈剂量依赖性,另外,gingerol对内皮完整的胸主动脉的舒张作用较去内皮的胸主动脉明显增强(P＜0.05).(2)内皮完整的胸主动脉组,L-NAME能显著抑制gingerol对胸主动脉平滑肌的舒张作用(％,NE处理组:94.83±3.63比96.83±1.82、91.17 ±2.89比94.83±2.68、83.83±2.23比94.67±2.73、78.00±3.54比92.33±2.91、67.83±2.37比90.83±3.77、62.83±3.42比89.67±3.15;KCl处理组:90.33±8.33比98.17±7.56、80.83±7.24比96.83±7.28、70.83±7.57比92.17±7.31、67.83±7.68比89.83±7.23、62.83±7.49比90.33±8.87、57.33±8.32比87.67±8.97,P＜0.05).(3)内皮完整的胸主动脉组吲哚美辛能显著抑制gingerol对胸主动脉平滑肌的舒张作用(％,NE处理组:93.83±7.61比97.83±7.45、92.50±10.13比95.33±8.36、86.67±8.24比93.83±7.28、79.83±7.36比89.83±7.63、67.83±7.46比85.33±8.94、61.83 ±7.55比81.83 ±7.25;KC1处理组:90.17±7.77比96.17±7.31、79.67±8.65比91.33±8.62、72.17 ±7.48比85.67±8.76、70.83±7.78比83.17±7.79、56.83±7.62比77.83±7.29、54.17 ±7.68比74.83±7.34,P＜0.05).结论 gingerol对NE和KCl所诱发的收缩具有剂量依赖性的抑制作用,其机制可能与内皮产生的NO和前列环素有关.
    목적 관찰강분(gingerol)대가토흉주동맥평활기적서장작용병탐토기작용궤제.방법 제비리체내피완정적화거내피흉주동맥배기조,현괘우항온욕조중,병장기여장력-위이환능기상련기록혈관배기조장력적변화,소유표본분위내피완정조화거내피조.내피완정조분위1×10-5 mol/L거갑신상선소(NE)+불동농도gingerol조(1×10-8、1 ×10-7、1 ×10-6、1 ×10-5、1×10-4、1×10-3 mol/L,하동)、20 mmol/L KCl+불동농도gingerol조、1×10-5 mol/L NE+좌선-초기정안산갑지(L-NAME)+불동농도gingerol조、1×10-5 mol/L NE+신타미신+불동농도gingerol조、20 mmol/LKCl+ L-NAME+불동농도gingerol조、20 mmol/L KCl+신타미신+불동농도gingerol조;거내피조분위1×10-5 mol/L NE+불동농도gingerol조、20 mmol/L KCl+불동농도gingerol조.결과 (1)gingerol능억제1×10-5 mol/L적NE혹20 mmol/L적KCl인기적흉주동맥평활기수축병정제량의뢰성,령외,gingerol대내피완정적흉주동맥적서장작용교거내피적흉주동맥명현증강(P＜0.05).(2)내피완정적흉주동맥조,L-NAME능현저억제gingerol대흉주동맥평활기적서장작용(％,NE처리조:94.83±3.63비96.83±1.82、91.17 ±2.89비94.83±2.68、83.83±2.23비94.67±2.73、78.00±3.54비92.33±2.91、67.83±2.37비90.83±3.77、62.83±3.42비89.67±3.15;KCl처리조:90.33±8.33비98.17±7.56、80.83±7.24비96.83±7.28、70.83±7.57비92.17±7.31、67.83±7.68비89.83±7.23、62.83±7.49비90.33±8.87、57.33±8.32비87.67±8.97,P＜0.05).(3)내피완정적흉주동맥조신타미신능현저억제gingerol대흉주동맥평활기적서장작용(％,NE처리조:93.83±7.61비97.83±7.45、92.50±10.13비95.33±8.36、86.67±8.24비93.83±7.28、79.83±7.36비89.83±7.63、67.83±7.46비85.33±8.94、61.83 ±7.55비81.83 ±7.25;KC1처리조:90.17±7.77비96.17±7.31、79.67±8.65비91.33±8.62、72.17 ±7.48비85.67±8.76、70.83±7.78비83.17±7.79、56.83±7.62비77.83±7.29、54.17 ±7.68비74.83±7.34,P＜0.05).결론 gingerol대NE화KCl소유발적수축구유제량의뢰성적억제작용,기궤제가능여내피산생적NO화전렬배소유관.
    Objective To observe the effect of gingerol on the isolated thoracic aorta ring of rabbits and investigate its underlying mechanisms.Methods The thoracic aortic ring muscle strips with intact and denuded endothelium were prepared and placed in an organ chamber containing physiological salt solution (PSS).Every strip was connected to the tension transducer and the change of the vascular ring tension was recorded and analyzed by the biological signal collection and analysis computer system.Th isotonic tension of thoracic aortic tings with intact and denuded endothelium precontracted by norepinephrine (NE,1 × 10-5 mol/L) or KCl (20 mmol/L) was recorded before and after administrating gingerol.Meanwhile,we also observed the effects of N-nitro-L-arginine methyl ester (L-NAME,1 × 10-4 mol/L) and indomethacin (1 × 10-8 mol/L) on the action of gingerol in the rings.Results (1) Administration of 1 × 10-5 mol/L NE or 20 mmol/L KCl could produce the contraction effect in the thoracic aortic rings with intact endothelium.gingerol induced the muscle relaxation of aorta in both intact and denuded endothelium groups in a dose-dependant manner,meanwhile,the relaxation effect of gingerol was stronger in intact endothelium group than that in denuded endothelium group (P ＜ 0.05).(2) L-NAME could inhibit the vasodilative role caused by gingerol in intact endothelium group (％,94.83 ±3.63 vs.96.83 ± 1.82,91.17 ± 2.89 vs.94.83 ± 2.68,83.83 ± 2.23 vs.94.67 ± 2.7,78.00 ± 3.54 vs.92.33 ± 2.91,67.83 ± 2.37 vs.90.83 ±3.77,62.83 ±3.42 vs.89.67 ±3.15 respectively in NE-treated group and 90.33 ± 8.33 vs.98.17±7.56,80.83 ±7.24 vs.96.83 ±7.28,70.83±7.57 vs.92.17±7.31,67.83 ±7.68 vs.89.83 ± 7.23,62.83 ± 7.49 vs.90.33 ± 8.87,57.33 ± 8.32 vs.87.67 ± 8.97 respectively in KCl-treated group,P ＜ 0.05).(3) Indomethacin could inhibit the vasodilative role caused by gingerol in intact endothelium group (％,93.83 ± 7.61 vs.97.83 ± 7.45,92.50 ± 10.13 vs.95.33 ± 8.36,86.67 ± 8.24 vs.93.83 ± 7.28,79.83 ± 7.36 vs.89.83 ± 7.63,67.83 ± 7.46 vs.85.33 ± 8.94,61.83 ± 7.55 vs.81.83 ±7.25 respectively in NE-treated group and 90.17 ±7.77 vs.96.17 ±7.31,79.67 ±8.65 vs.91.33 ±8.62,72.17 ±7.48 vs.85.67 ±8.76,70.83 ±7.78 vs.83.17 ±7.79,56.83 ±7.62 vs.77.83 ±7.29,54.17 ±7.68 vs.74.83 ±7.34 respectively in KCl-treated group,P＜0.05).Conclusion (1) Gingero preferentially antagonized the contraction effect induced by NE or KCl in a dose-dependent manner.(2) The vasorelaxation effect of gingerol on the thoracic aortic ring muscle strips was relevant to the production of NO and prostacyclin.
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