中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2015年
1期
78-80
,共3页
异常腺窝病灶%大肠癌%表达
異常腺窩病竈%大腸癌%錶達
이상선와병조%대장암%표체
Aberrant crypt foci%Colorectal cancer%Expression
目的 观察Wistar鼠大肠癌(CRC)模型中异常腺窝病灶(ACF)的发展,探讨ACF与肿瘤的相关性.方法 将成年Wistar大鼠60只随机分为2组,A组中30只Wistar大白鼠给予二甲肼(DMH)腹腔注射(20 mg/kg,每周1次,连续18周),B组大鼠腹腔内每周注射生理盐水,作为正常对照组.将A,B组大鼠随机分为3个亚组,于第8、16和24周分批处死,将亚甲蓝染色后的大肠组织在实体镜下观察.结果 ACF均分布于中段及远端大肠(大部分位距盲肠50％ ～ 80％处),可见肿瘤12个,其中4个肿瘤为低分化黏液腺癌,均分布在近端结肠(距盲肠10％～20％).另外8个肿瘤中有3个为腺瘤,5个为高分化腺癌,均分布在距盲肠50％ ～ 80％处.结直肠中的ACF数目对结直肠肿瘤的发生有显著影响.A组中结直肠有ACF的大鼠其病理学切片显示有46.67％(14/30)可见结肠病变,有16.67％ (5/30)可见结直肠肿瘤,其概率较无ACF大鼠明显升高,其比值比(OR)[95％可信区间(CI)]分别为13.352(5.529 ～ 32.581),6.582(1.374 ～ 17.494),其差异均有统计学意义(P＜0.05).结论 结直肠中的ACF数目对结直肠肿瘤的发生有显著影响.
目的 觀察Wistar鼠大腸癌(CRC)模型中異常腺窩病竈(ACF)的髮展,探討ACF與腫瘤的相關性.方法 將成年Wistar大鼠60隻隨機分為2組,A組中30隻Wistar大白鼠給予二甲肼(DMH)腹腔註射(20 mg/kg,每週1次,連續18週),B組大鼠腹腔內每週註射生理鹽水,作為正常對照組.將A,B組大鼠隨機分為3箇亞組,于第8、16和24週分批處死,將亞甲藍染色後的大腸組織在實體鏡下觀察.結果 ACF均分佈于中段及遠耑大腸(大部分位距盲腸50％ ～ 80％處),可見腫瘤12箇,其中4箇腫瘤為低分化黏液腺癌,均分佈在近耑結腸(距盲腸10％～20％).另外8箇腫瘤中有3箇為腺瘤,5箇為高分化腺癌,均分佈在距盲腸50％ ～ 80％處.結直腸中的ACF數目對結直腸腫瘤的髮生有顯著影響.A組中結直腸有ACF的大鼠其病理學切片顯示有46.67％(14/30)可見結腸病變,有16.67％ (5/30)可見結直腸腫瘤,其概率較無ACF大鼠明顯升高,其比值比(OR)[95％可信區間(CI)]分彆為13.352(5.529 ～ 32.581),6.582(1.374 ～ 17.494),其差異均有統計學意義(P＜0.05).結論 結直腸中的ACF數目對結直腸腫瘤的髮生有顯著影響.
목적 관찰Wistar서대장암(CRC)모형중이상선와병조(ACF)적발전,탐토ACF여종류적상관성.방법 장성년Wistar대서60지수궤분위2조,A조중30지Wistar대백서급여이갑정(DMH)복강주사(20 mg/kg,매주1차,련속18주),B조대서복강내매주주사생리염수,작위정상대조조.장A,B조대서수궤분위3개아조,우제8、16화24주분비처사,장아갑람염색후적대장조직재실체경하관찰.결과 ACF균분포우중단급원단대장(대부분위거맹장50％ ～ 80％처),가견종류12개,기중4개종류위저분화점액선암,균분포재근단결장(거맹장10％～20％).령외8개종류중유3개위선류,5개위고분화선암,균분포재거맹장50％ ～ 80％처.결직장중적ACF수목대결직장종류적발생유현저영향.A조중결직장유ACF적대서기병이학절편현시유46.67％(14/30)가견결장병변,유16.67％ (5/30)가견결직장종류,기개솔교무ACF대서명현승고,기비치비(OR)[95％가신구간(CI)]분별위13.352(5.529 ～ 32.581),6.582(1.374 ～ 17.494),기차이균유통계학의의(P＜0.05).결론 결직장중적ACF수목대결직장종류적발생유현저영향.
Objective To observe the development of aberrant crypt foci (ACF) in Wistar rat colon cancer model and to evaluate the correlation between ACF and tumor.Methods Sixty adult Wistar rats were randomly divided into 2 groups.Thirty Wistar rats in group A were subjected to the subcutaneous injection of 1,2-dimethylhydrazine (DMH) (20 mg/kg,once every week for 18 weeks),and those in group B were intraperitoneally injected with physiological saline for one week as the normal control group.The rats in groups A and B group were killed at 6th,12th and 18th week respectively,and served as three subgroups.The colon tissues subject to methylene blue staining were observed under a microscope.Results ACF were distributed in the middle and distal colon (mostly 50％-80％ of the cecum),and 12 tumors were seen.Four tumors were poorly differentiated mucinous adenocarcinoma,and located in the proximal colon (10％-20％ of the cecum).In the rest 8 tumors,three were adenoma and 5 highly differentiated adenocarcinoma,and located in 50％-80％ of the distal colon.The number of ACF in the colorectum could significantly influence the incidence of colorectal cancer.In group A of rats with ACF in colorectal,the pathological examination showed that the incidence of colonic lesions was 46.67％ (14/30) and that of rectal cancer was 16.67％ (5/30) respectively,which was significantly higher than in the rats without ACR odds ratio (OR) [95％ confidence interval (CI)]:13.352 (5.529-32.581) and6.582 (1.374-17.494),P ＜ 0.05].Conclusion The number of ACF in the colorectum has significant influence on the development of colorectal cancer.
