中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2015年
1期
97-99
,共3页
万鸿君%姚小兵%余伟民%程帆%李文
萬鴻君%姚小兵%餘偉民%程帆%李文
만홍군%요소병%여위민%정범%리문
肾积水%灌注压力%线粒体渗透性转换孔%损伤
腎積水%灌註壓力%線粒體滲透性轉換孔%損傷
신적수%관주압력%선립체삼투성전환공%손상
Hydronephrosis%Irrigation pressure%Mitochondrial permeability transition pore%Damage
目的 在不同程度肾脏积水的前提下,观察线粒体渗透性转换孔(MPTP)抑制剂环孢素A(CsA)对于急性压力灌注所致损伤的保护作用.方法 健康新西兰大白兔20只,随机分为轻度积水组(M组,n=10)和重度积水组(S组,n=10),肾脏轻度积水及重度积水模型采用输尿管套扎法构建.M组及S组分为M1、M2、S1、S24个亚组,分别予以60、100、20、60 mmHg(1mmHg=0.133 kPa)压力灌注.灌注前M组及S组均给予CsA灌胃1周.灌注后48 h取标本,检测标本中超氧化物歧化酶(SOD)及丙二醛(MDA)的含量,JC-1染色检测线粒体膜电位(MMP)的变化.结果 M1、M2、S1、S2各组SOD活力分别为(30.11±2.23)、(28.41 ±2.26)、(44.82±6.61)、(40.31±5.21) U/mg,M1与M2之间SOD活力差异无统计学意义(P＞0.05),S1与S2之间SOD活力差异无统计学意义(P＞0.05);MDA的含量分别为(0.61 ±0.17)、(0.60±0.15)、(0.95 ±0.26)、(0.80±0.32) nmol/mg,M1与M2之间MDA含量差异无统计学意义(P＞0.05),S1与S2之间MDA含量差异无统计学意义(P＞0.05).JC-1染色橙红色阳性细胞百分比分别为(67.16±9.47)％、(60.75±11.89)％、(41.08 ±4.50)％、(42.36 ±3.44)％.M1与M2比较,S1与S2比较,阳性细胞百分比差异均无统计学意义(P＞0.05).结论 CsA对于积水肾脏因急性压力灌注所致的损伤有一定的保护作用.在经皮肾镜及输尿管镜治疗结石前给予MPTP抑制剂可有效减轻压力灌注所致损伤.
目的 在不同程度腎髒積水的前提下,觀察線粒體滲透性轉換孔(MPTP)抑製劑環孢素A(CsA)對于急性壓力灌註所緻損傷的保護作用.方法 健康新西蘭大白兔20隻,隨機分為輕度積水組(M組,n=10)和重度積水組(S組,n=10),腎髒輕度積水及重度積水模型採用輸尿管套扎法構建.M組及S組分為M1、M2、S1、S24箇亞組,分彆予以60、100、20、60 mmHg(1mmHg=0.133 kPa)壓力灌註.灌註前M組及S組均給予CsA灌胃1週.灌註後48 h取標本,檢測標本中超氧化物歧化酶(SOD)及丙二醛(MDA)的含量,JC-1染色檢測線粒體膜電位(MMP)的變化.結果 M1、M2、S1、S2各組SOD活力分彆為(30.11±2.23)、(28.41 ±2.26)、(44.82±6.61)、(40.31±5.21) U/mg,M1與M2之間SOD活力差異無統計學意義(P＞0.05),S1與S2之間SOD活力差異無統計學意義(P＞0.05);MDA的含量分彆為(0.61 ±0.17)、(0.60±0.15)、(0.95 ±0.26)、(0.80±0.32) nmol/mg,M1與M2之間MDA含量差異無統計學意義(P＞0.05),S1與S2之間MDA含量差異無統計學意義(P＞0.05).JC-1染色橙紅色暘性細胞百分比分彆為(67.16±9.47)％、(60.75±11.89)％、(41.08 ±4.50)％、(42.36 ±3.44)％.M1與M2比較,S1與S2比較,暘性細胞百分比差異均無統計學意義(P＞0.05).結論 CsA對于積水腎髒因急性壓力灌註所緻的損傷有一定的保護作用.在經皮腎鏡及輸尿管鏡治療結石前給予MPTP抑製劑可有效減輕壓力灌註所緻損傷.
목적 재불동정도신장적수적전제하,관찰선립체삼투성전환공(MPTP)억제제배포소A(CsA)대우급성압력관주소치손상적보호작용.방법 건강신서란대백토20지,수궤분위경도적수조(M조,n=10)화중도적수조(S조,n=10),신장경도적수급중도적수모형채용수뇨관투찰법구건.M조급S조분위M1、M2、S1、S24개아조,분별여이60、100、20、60 mmHg(1mmHg=0.133 kPa)압력관주.관주전M조급S조균급여CsA관위1주.관주후48 h취표본,검측표본중초양화물기화매(SOD)급병이철(MDA)적함량,JC-1염색검측선립체막전위(MMP)적변화.결과 M1、M2、S1、S2각조SOD활력분별위(30.11±2.23)、(28.41 ±2.26)、(44.82±6.61)、(40.31±5.21) U/mg,M1여M2지간SOD활력차이무통계학의의(P＞0.05),S1여S2지간SOD활력차이무통계학의의(P＞0.05);MDA적함량분별위(0.61 ±0.17)、(0.60±0.15)、(0.95 ±0.26)、(0.80±0.32) nmol/mg,M1여M2지간MDA함량차이무통계학의의(P＞0.05),S1여S2지간MDA함량차이무통계학의의(P＞0.05).JC-1염색등홍색양성세포백분비분별위(67.16±9.47)％、(60.75±11.89)％、(41.08 ±4.50)％、(42.36 ±3.44)％.M1여M2비교,S1여S2비교,양성세포백분비차이균무통계학의의(P＞0.05).결론 CsA대우적수신장인급성압력관주소치적손상유일정적보호작용.재경피신경급수뇨관경치료결석전급여MPTP억제제가유효감경압력관주소치손상.
Objective To study the protective effect of cyclosporine A for acute renal function impairment induced by various of irrigation pressures in mild and severe hydronephrosis and to provided a reference values for clinical work during endourological procedures.Methods A total 20 rabbits were randomly divided into two groups:Mild hydronephrosis group (group M,n =10),Severe hydronephrosis group (group S,n =10).Both mild and severe hydronephrosis model were induced by embeding ureters into psoas.In each group,rabbits were randomly divided into 2 subgroups (M1,M2,S1 and S2) of respectively 5 rabbits.Groups M1,M2,S1 and S2 were respectively perfused with 60,100,20 and 100 mmHg (1 mmHg =0.133 kPa) pressure fluid.M and S groups were treated with CsA administered for one week before perfusion.The prefusion time was lasted for one hour.After that,the kidneys were harvested for measurement of super oxide dismutase (SOD) and malonaldehyde (MDA).And the change of mitochondrial membrane potential was detected by JC-1 staining flow cytometry.Resets For group M1,M2,S1 and S2,SOD were (30.11 ±2.23),(28.41 ±2.26),(44.82 ±6.61),(40.31 ±5.21) U/mg respectively; MDA were (0.61 ±0.17),(0.60 ±0.15),(0.95 ±0.26),(0.80 ±0.32) nmol/mg respectively; the percentage of positive cell of expressing JC-1 were (67.16 ± 9.47)％,(60.75 ±11.89)％,(41.08 ±4.50)％,(42.36 ±3.44)％ respectively.For M group,SOD,MDA and the percentage of positive cell has no significantly differences ; For S group,no abnormalities were observed.Conchsion CsA has a protective effect for damage due to renal perfusion.Give CsA before endosurgical procedures can effectively decrease damages induced by various of irrigation pressures.
