CAJ | 학술논문

目的观察小分子抑制剂Spautin-1对骨肉瘤细胞株(U2OS)化疗敏感性的影响.方法培养U2OS细胞,使用有效自噬抑制浓度的Spautin-1(10 μmol/L)和顺铂(5μmol/L)处理U2OS,细胞计数试剂盒(CCK-8)法检测Spautin-1对于顺铂(Cisplatin)介导的U2OS的增殖抑制作用的影响,流式细胞术和Western blot检测Spautin-1对顺铂介导的U2OS的凋亡诱导作用的影响.结果有效自噬抑制浓度的Spautin-1(10 μmol/L)对U2OS无明显的生长抑制及凋亡诱导作用(P＞0.05),而此浓度的Spautin-1可明显增强顺铂对U2OS的增殖抑制(P＜0.05),与顺铂处理组(16.5％)比较,Spautin-1与顺铂共处理后U2OS细胞凋亡比例明显增加(35.0％),切割后的半胱氨酰天冬氨酸特异性蛋白酶-3(Caspase-3)和聚腺苷二磷酸核糖聚合酶(PARP)明显升高.结论小分子抑制剂Spautin-1可增强U2OS对顺铂的敏感性.
목적 관찰소분자억제제Spautin-1대골육류세포주(U2OS)화료민감성적영향.방법 배양U2OS세포,사용유효자서억제농도적Spautin-1(10 μmol/L)화순박(5μmol/L)처리U2OS,세포계수시제합(CCK-8)법검측Spautin-1대우순박(Cisplatin)개도적U2OS적증식억제작용적영향,류식세포술화Western blot검측Spautin-1대순박개도적U2OS적조망유도작용적영향.결과 유효자서억제농도적Spautin-1(10 μmol/L)대U2OS무명현적생장억제급조망유도작용(P＞0.05),이차농도적Spautin-1가명현증강순박대U2OS적증식억제(P＜0.05),여순박처리조(16.5％)비교,Spautin-1여순박공처리후U2OS세포조망비례명현증가(35.0％),절할후적반광안선천동안산특이성단백매-3(Caspase-3)화취선감이린산핵당취합매(PARP)명현승고.결론 소분자억제제Spautin-1가증강U2OS대순박적민감성.
Objective To study the effect of autophagy inhibitor Spautin-1 on osteosarcoma cell sensitivity to cisplatin-induced cell proliferation inhibition and apoptosis.Methods Osteosarcoma cell line U2OS cells were treated with an effective inhibition concentration of Spautin-1 (10 μmol/L).Cell proliferation was measured by cell counting kit-8 (CCK-8),and cell apoptosis was measured by flow cytometry and Western blotting.Results Spautin-1 has no obvious effect on U2OS cell proliferation and apoptosis (P ＞ 0.05).However,Spautin-1 sensitized osteosarcoma cells to cisplatin-induced cell proliferation inhibition and apoptosis.The flow cytometry indicated that the apoptosis rate in Spautin-1 + cisplatin group (35.0％) was significantly higher than that in cisplatin group (16.5％).The cleaved Caspase-3 and poly adenosine diphosphate-ribose polymerase (PARP) were also increased in Spautin-1 +cisplating group as compared with cisplatin group.Conclusion The autophagy inhibitor Spautin-1 sensitizes osteosarcoma cells to cisplatin-induced cell proliferation inhibition and apoptosis.