中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2015年
1期
139-141
,共3页
周围神经损伤%糖尿病%神经生长因子%钙通道阻滞剂
週圍神經損傷%糖尿病%神經生長因子%鈣通道阻滯劑
주위신경손상%당뇨병%신경생장인자%개통도조체제
Peripheral nerve injury%Diabetes mellitus%Nerve growth factor%Calcium channel blockers
目的 比较神经生长因子(NGF)与尼莫地平两种药物对大鼠糖尿病周围神经病变(DPN)预处理的影响.方法 用链脲佐菌素(STZ)诱导建立糖尿病(DM)大鼠模型并随机分组:Ⅰ:空白对照组(Control);Ⅱ:糖尿病组(DM);Ⅲ:糖尿病+β-神经生长因子组(DM+ β-NGF);Ⅳ:糖尿病+尼莫地平组(DM+ Nimodipine).检测各组坐骨神经运动传导速度(MNCV)以及坐骨神经病理改变.结果 坐骨神经MNCV的测量结果:8周时,Control组[(50.70±1.12) m/s]与DM组[(45.80±1.12) m/s]比较,差异有统计学意义(P＜0.05),β-NGF组[(49.50±1.08) m/s]、尼莫地平组[(48.50±0.81) m/s]分别与DM组[(45.80±1.12) m/s]比较,差异有统计学意义(P＜0.05);12周时,β-NGF组[(49.50±1.08) m/s]、尼莫地平组[(48.50±0.81) m/s]分别与Control组[(50.90±1.31) m/s]比较,差异有统计学意义(P＜0.05),β-NGF组[(49.50±1.08) m/s]与尼莫地平组[(48.50±0.81) m/s]比较,差异有统计学意义(P＜0.05).与Control比较,其余3组坐骨神经形态学结构均有不同程度的破坏,DM组＞尼莫地平组＞β-NGF组.结论 NGF和尼莫地平均对大鼠坐骨神经糖尿病周围神经病变有预保护作用,但不能阻止其发生和发展,NGF对其预保护作用要优于尼莫地平,并随时间延长效果也逐渐明显.
目的 比較神經生長因子(NGF)與尼莫地平兩種藥物對大鼠糖尿病週圍神經病變(DPN)預處理的影響.方法 用鏈脲佐菌素(STZ)誘導建立糖尿病(DM)大鼠模型併隨機分組:Ⅰ:空白對照組(Control);Ⅱ:糖尿病組(DM);Ⅲ:糖尿病+β-神經生長因子組(DM+ β-NGF);Ⅳ:糖尿病+尼莫地平組(DM+ Nimodipine).檢測各組坐骨神經運動傳導速度(MNCV)以及坐骨神經病理改變.結果 坐骨神經MNCV的測量結果:8週時,Control組[(50.70±1.12) m/s]與DM組[(45.80±1.12) m/s]比較,差異有統計學意義(P＜0.05),β-NGF組[(49.50±1.08) m/s]、尼莫地平組[(48.50±0.81) m/s]分彆與DM組[(45.80±1.12) m/s]比較,差異有統計學意義(P＜0.05);12週時,β-NGF組[(49.50±1.08) m/s]、尼莫地平組[(48.50±0.81) m/s]分彆與Control組[(50.90±1.31) m/s]比較,差異有統計學意義(P＜0.05),β-NGF組[(49.50±1.08) m/s]與尼莫地平組[(48.50±0.81) m/s]比較,差異有統計學意義(P＜0.05).與Control比較,其餘3組坐骨神經形態學結構均有不同程度的破壞,DM組＞尼莫地平組＞β-NGF組.結論 NGF和尼莫地平均對大鼠坐骨神經糖尿病週圍神經病變有預保護作用,但不能阻止其髮生和髮展,NGF對其預保護作用要優于尼莫地平,併隨時間延長效果也逐漸明顯.
목적 비교신경생장인자(NGF)여니막지평량충약물대대서당뇨병주위신경병변(DPN)예처리적영향.방법 용련뇨좌균소(STZ)유도건립당뇨병(DM)대서모형병수궤분조:Ⅰ:공백대조조(Control);Ⅱ:당뇨병조(DM);Ⅲ:당뇨병+β-신경생장인자조(DM+ β-NGF);Ⅳ:당뇨병+니막지평조(DM+ Nimodipine).검측각조좌골신경운동전도속도(MNCV)이급좌골신경병리개변.결과 좌골신경MNCV적측량결과:8주시,Control조[(50.70±1.12) m/s]여DM조[(45.80±1.12) m/s]비교,차이유통계학의의(P＜0.05),β-NGF조[(49.50±1.08) m/s]、니막지평조[(48.50±0.81) m/s]분별여DM조[(45.80±1.12) m/s]비교,차이유통계학의의(P＜0.05);12주시,β-NGF조[(49.50±1.08) m/s]、니막지평조[(48.50±0.81) m/s]분별여Control조[(50.90±1.31) m/s]비교,차이유통계학의의(P＜0.05),β-NGF조[(49.50±1.08) m/s]여니막지평조[(48.50±0.81) m/s]비교,차이유통계학의의(P＜0.05).여Control비교,기여3조좌골신경형태학결구균유불동정도적파배,DM조＞니막지평조＞β-NGF조.결론 NGF화니막지평균대대서좌골신경당뇨병주위신경병변유예보호작용,단불능조지기발생화발전,NGF대기예보호작용요우우니막지평,병수시간연장효과야축점명현.
Objective The present study was planned to compare the nerve growth factor (NGF)and nimodipine on diabetic peripheral neuropathy (DPN) the effects of pretreatment.Methods The diabetic rats model that induced by streptozotocin (STZ) were randomly divided into groups:Ⅰ:blank group (Control) ; Ⅱ:diabetes mellitus group (DM) ; Ⅲ:diabetes + β-NGF group (DM + β-NGF) ; Ⅳ:diabetes + nimodipine group (DM + Nimodipine).The level of sciatic morter nerve conduction velocity and pathology of the animals were checked.Results At 8 week,compared with control group [(50.70 ±1.12) m/s],the MNCV of DM group [(45.80 ± 1.12) m/s] decreased significantly (P ＜ 0.05).β-NGF group [(49.50 ± 1.08) m/s] and nimodipine group [(48.50 ±0.81) m/s] compared with the DM group [(45.80 ± 1.12) m/s] was statistically significant (P ＜ 0.05) ; At 12 week,compared with Control group [(43.90 ± 1.03) m/s],β-NGF group [(49.50 ± 1.08) m/s] and Nimodipine group [(48.50 ± 0.81) m/s] was statistically significant (P ＜ 0.05),Compared with Nimodipine group [(48.50 ±0.81) m/s],β-NGF group [(49.50 ± 1.08) m/s] has better protection to the nerve and there is obviously difference (P ＜ 0.05).Compared with Control group,the structure of the sciatic nerve was damaged in different degree.β-NGF group show little damage than nimodipine group,and the sciatic structure of DM group is the worst.Conclusion Nerve growth factor and nimodipine has protective effect on sciatic nerve in diabetic rats,but cannot prevent the occurrence and development.Protective effect of NGF on its superior to Nimodipine,and extended the effects become apparent over time.
