中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2015年
1期
142-143
,共2页
姜迎海%夏令杰%刘琳%李海芹
薑迎海%夏令傑%劉琳%李海芹
강영해%하령걸%류림%리해근
米贝地尔%神经病理性疼痛
米貝地爾%神經病理性疼痛
미패지이%신경병이성동통
Mibefradil%Neuropathic pain
目的 观察米贝地尔对糖尿病神经病理性疼痛(DNP)大鼠的镇痛作用.方法 制备DNP模型大鼠21只,随机分为3组(n=7):A组:腹腔内注射生理盐水(NS)3 ml;B组:腹腔内注射米贝地尔0.5 mg;C组:腹腔内注射米贝地尔1.5 mg.测定给药前、给药后0.5、1.0、2.0、4.0、8.0h大鼠机械性缩足反射阈值(MWT)和热缩足潜伏期(TWL).结果 各时间点B组大鼠MWT[0.5 h:(6.2±2.3)g;1.0 h:(9.7±3.1)g;2.0 h:(8.8±3.4)g;4.0 h:(7.2±2.7)g;8.0 h:(4.4±0.4)g],TWL[0.5 h:(12.2±2.1)s;1.0 h:(13.9 ±2.3)s;2.0 h:(13.2±2.4)s;4.0 h:(11.9±2.2)s;8.0 h:(9.9±0.9)s],均较A组明显增加(P＜0.05);各时间点C组大鼠MWT[0.5 h:(10.6 ±3.1)g;1.0h:(12.9±2.7) g;2.0h:(10.9±4.2) g;4.0h:(9.2±3.1)g;8.0h:(5.2±0.6) g],TWL[0.5 h:(14.6±3.0)s;1.0h:(17.1±3.2)s;2.0 h:(16.5±3.6)s;4.0 h:(13.0±3.4)s;8.0 h:(10.9±2.3)s],均较A组明显增加(P＜0.01).结论 腹腔内注射米贝地尔对DNP大鼠有较好的镇痛作用.
目的 觀察米貝地爾對糖尿病神經病理性疼痛(DNP)大鼠的鎮痛作用.方法 製備DNP模型大鼠21隻,隨機分為3組(n=7):A組:腹腔內註射生理鹽水(NS)3 ml;B組:腹腔內註射米貝地爾0.5 mg;C組:腹腔內註射米貝地爾1.5 mg.測定給藥前、給藥後0.5、1.0、2.0、4.0、8.0h大鼠機械性縮足反射閾值(MWT)和熱縮足潛伏期(TWL).結果 各時間點B組大鼠MWT[0.5 h:(6.2±2.3)g;1.0 h:(9.7±3.1)g;2.0 h:(8.8±3.4)g;4.0 h:(7.2±2.7)g;8.0 h:(4.4±0.4)g],TWL[0.5 h:(12.2±2.1)s;1.0 h:(13.9 ±2.3)s;2.0 h:(13.2±2.4)s;4.0 h:(11.9±2.2)s;8.0 h:(9.9±0.9)s],均較A組明顯增加(P＜0.05);各時間點C組大鼠MWT[0.5 h:(10.6 ±3.1)g;1.0h:(12.9±2.7) g;2.0h:(10.9±4.2) g;4.0h:(9.2±3.1)g;8.0h:(5.2±0.6) g],TWL[0.5 h:(14.6±3.0)s;1.0h:(17.1±3.2)s;2.0 h:(16.5±3.6)s;4.0 h:(13.0±3.4)s;8.0 h:(10.9±2.3)s],均較A組明顯增加(P＜0.01).結論 腹腔內註射米貝地爾對DNP大鼠有較好的鎮痛作用.
목적 관찰미패지이대당뇨병신경병이성동통(DNP)대서적진통작용.방법 제비DNP모형대서21지,수궤분위3조(n=7):A조:복강내주사생리염수(NS)3 ml;B조:복강내주사미패지이0.5 mg;C조:복강내주사미패지이1.5 mg.측정급약전、급약후0.5、1.0、2.0、4.0、8.0h대서궤계성축족반사역치(MWT)화열축족잠복기(TWL).결과 각시간점B조대서MWT[0.5 h:(6.2±2.3)g;1.0 h:(9.7±3.1)g;2.0 h:(8.8±3.4)g;4.0 h:(7.2±2.7)g;8.0 h:(4.4±0.4)g],TWL[0.5 h:(12.2±2.1)s;1.0 h:(13.9 ±2.3)s;2.0 h:(13.2±2.4)s;4.0 h:(11.9±2.2)s;8.0 h:(9.9±0.9)s],균교A조명현증가(P＜0.05);각시간점C조대서MWT[0.5 h:(10.6 ±3.1)g;1.0h:(12.9±2.7) g;2.0h:(10.9±4.2) g;4.0h:(9.2±3.1)g;8.0h:(5.2±0.6) g],TWL[0.5 h:(14.6±3.0)s;1.0h:(17.1±3.2)s;2.0 h:(16.5±3.6)s;4.0 h:(13.0±3.4)s;8.0 h:(10.9±2.3)s],균교A조명현증가(P＜0.01).결론 복강내주사미패지이대DNP대서유교호적진통작용.
Objective To evaluate the analgesic effect of mibefradil on diabetic neuropathic pain in rats.Methods Twenty-one rats were used to establish the models of diabetic neuropathic pain.The rats were randomly divided into 3 groups (n =7 each):group A,3 ml normal saline was intraperitoneally injected ; group B,0.5 mg mibefradil was intraperitoneally injected; group C,1.5 mg mibefradil was intraperitoneally given.The mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL)were measured before and 0.5,1.0,2.0,4.0 and 8.0 h after administration respectively.Results In groupB,MWTateach time point [0.5 h:(6.2±2.3) g; 1.0 h:(9.7±3.1) g; 2.0 h:(8.8±3.4) g;4.0h:(7.2±2.7)g; 8.0h:(4.4±0.4) g],andTWL [0.5 h:(12.2±2.1) s; 1.0 h:(13.9±2.3) s; 2.0 h:(13.2±2.4) s; 4.0 h:(11.9±2.2) s; 8.0 h:(9.9±0.9) s] were significantly increased as compare with those in group A (P ＜ 0.05).In group C,MWT at each time point [0.5 h:(10.6±3.1) g; 1.0 h:(12.9 ±2.7) g; 2.0 h:(10.9 ±4.2) g; 4.0 h:(9.2 ±3.1) g;8.0 h:(5.2±0.6) g],and TWL [0.5 h:(14.6±3.0) s; 1.0 h:(17.1 ±3.2) s; 2.0 h:(16.5 ±3.6) s ; 4.0 h:(13.0 ± 3.4) s ; 8.0 h:(10.9 ± 2.3) s] were singnificantly increased as compared with those in group A (P ＜ 0.01).Conclusion Mibefradil administrered intraperitioneally can produce a better analgesic effect in rats model of diabetic neuropathic pain.
