中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2015年
1期
147-150
,共4页
陶凯%沈飞霞%陈景海%余玉慧%孙慧艳
陶凱%瀋飛霞%陳景海%餘玉慧%孫慧豔
도개%침비하%진경해%여옥혜%손혜염
辅助性T细胞22%白细胞介素-22%甲状腺肿瘤
輔助性T細胞22%白細胞介素-22%甲狀腺腫瘤
보조성T세포22%백세포개소-22%갑상선종류
T helper 22 cells%Interleukin 22%Thyroid tumor
目的 检测甲状腺肿瘤患者外周血及肿瘤组织中辅助性T细胞22 (Th22)及白细胞介素(IL)-22的表达水平,探讨其与患者临床病理特征的关系.方法 选择56例原发甲状腺肿瘤患者和30例健康志愿者,采用流式细胞仪检测外周血Th22细胞水平,酶联免疫吸附试验法(ELISA)检测血清中IL-22表达水平,应用免疫组织化学双染色分析正常组织及肿瘤组织中Th22阳性细胞的浸润程度,分析这些参数与临床病理特征间的关系.结果 甲状腺肿瘤患者外周血中Th22细胞及IL-22水平分别为(1.74±0.27)％和(162.7±32.2) ng/L,显著高于对照组[(0.57±0.14)％,t=15.72,P＜0.01;(64.2 ±20.7) ng/L,t=11.31,P＜0.01];甲状腺癌患者的Th22细胞比例及IL-22水平明显高于甲状腺瘤患者[(2.21±0.34)％比(1.39±0.22)％,t=9.14,P＜0.01;(199.1±34.1)ng/L比(129.8±31.3) ng/L,t=6.79,P＜0.01].甲状腺肿瘤患者组织中Th22阳性细胞浸润率为56.25％,而对应正常组织为8.33％,差异有统计学意义(x2=8.167,P＜0.01);甲状腺癌组织中Th22细胞浸润率为78.6％,甲状腺瘤组织为38.9％,差异有统计学意义(x2=5.039,P＜0.05).外周血Th22细胞比例、IL-22的表达水平和肿瘤组织中Th22阳性细胞浸润程度与临床分期、淋巴结转明显相关(P＜0.05),与年龄、性别、病理类型无明显相关(P＞0.05).结论 甲状腺肿瘤患者外周血Th22细胞比例及血浆中IL-22水平显著升高,且与临床分期等病理特征明显相关,表明Th22细胞及IL-22参与甲状腺肿瘤的发生发展.
目的 檢測甲狀腺腫瘤患者外週血及腫瘤組織中輔助性T細胞22 (Th22)及白細胞介素(IL)-22的錶達水平,探討其與患者臨床病理特徵的關繫.方法 選擇56例原髮甲狀腺腫瘤患者和30例健康誌願者,採用流式細胞儀檢測外週血Th22細胞水平,酶聯免疫吸附試驗法(ELISA)檢測血清中IL-22錶達水平,應用免疫組織化學雙染色分析正常組織及腫瘤組織中Th22暘性細胞的浸潤程度,分析這些參數與臨床病理特徵間的關繫.結果 甲狀腺腫瘤患者外週血中Th22細胞及IL-22水平分彆為(1.74±0.27)％和(162.7±32.2) ng/L,顯著高于對照組[(0.57±0.14)％,t=15.72,P＜0.01;(64.2 ±20.7) ng/L,t=11.31,P＜0.01];甲狀腺癌患者的Th22細胞比例及IL-22水平明顯高于甲狀腺瘤患者[(2.21±0.34)％比(1.39±0.22)％,t=9.14,P＜0.01;(199.1±34.1)ng/L比(129.8±31.3) ng/L,t=6.79,P＜0.01].甲狀腺腫瘤患者組織中Th22暘性細胞浸潤率為56.25％,而對應正常組織為8.33％,差異有統計學意義(x2=8.167,P＜0.01);甲狀腺癌組織中Th22細胞浸潤率為78.6％,甲狀腺瘤組織為38.9％,差異有統計學意義(x2=5.039,P＜0.05).外週血Th22細胞比例、IL-22的錶達水平和腫瘤組織中Th22暘性細胞浸潤程度與臨床分期、淋巴結轉明顯相關(P＜0.05),與年齡、性彆、病理類型無明顯相關(P＞0.05).結論 甲狀腺腫瘤患者外週血Th22細胞比例及血漿中IL-22水平顯著升高,且與臨床分期等病理特徵明顯相關,錶明Th22細胞及IL-22參與甲狀腺腫瘤的髮生髮展.
목적 검측갑상선종류환자외주혈급종류조직중보조성T세포22 (Th22)급백세포개소(IL)-22적표체수평,탐토기여환자림상병리특정적관계.방법 선택56례원발갑상선종류환자화30례건강지원자,채용류식세포의검측외주혈Th22세포수평,매련면역흡부시험법(ELISA)검측혈청중IL-22표체수평,응용면역조직화학쌍염색분석정상조직급종류조직중Th22양성세포적침윤정도,분석저사삼수여림상병리특정간적관계.결과 갑상선종류환자외주혈중Th22세포급IL-22수평분별위(1.74±0.27)％화(162.7±32.2) ng/L,현저고우대조조[(0.57±0.14)％,t=15.72,P＜0.01;(64.2 ±20.7) ng/L,t=11.31,P＜0.01];갑상선암환자적Th22세포비례급IL-22수평명현고우갑상선류환자[(2.21±0.34)％비(1.39±0.22)％,t=9.14,P＜0.01;(199.1±34.1)ng/L비(129.8±31.3) ng/L,t=6.79,P＜0.01].갑상선종류환자조직중Th22양성세포침윤솔위56.25％,이대응정상조직위8.33％,차이유통계학의의(x2=8.167,P＜0.01);갑상선암조직중Th22세포침윤솔위78.6％,갑상선류조직위38.9％,차이유통계학의의(x2=5.039,P＜0.05).외주혈Th22세포비례、IL-22적표체수평화종류조직중Th22양성세포침윤정도여림상분기、림파결전명현상관(P＜0.05),여년령、성별、병리류형무명현상관(P＞0.05).결론 갑상선종류환자외주혈Th22세포비례급혈장중IL-22수평현저승고,차여림상분기등병리특정명현상관,표명Th22세포급IL-22삼여갑상선종류적발생발전.
Objective To detect the expression of T helper cells 22 (Th22) in the peripheral blood and the level of interleukin (IL)-22 in the plasma and the Th22 cells in the tumor tissues of patients with thyroid tumor,and to analyze the relationship between Th22/IL-22 and clinical characteristics in the pathogenesis of laryngeal squamous cell carcinoma (LSCC).Methods Fifty-six patients with thyroid tumor,and 30 healthy controls were included.Th22 cells in the peripheral blood were detected by flow cytometry,the concentrations of IL-22 in serum were measured by enzyme-linked immunosorbent assay (ELISA),Double immunohistochemistry (IHC) was used to determine the Th22 cells in paraffin-embedded tissues,the relationship between Th22/IL-22 and clinical characteristics were analyzed.Results The level of Th22 cells in peripheral blood and IL-22 in the serum of patients with thyroid tumor were (1.74 ± 0.27) ％ and (162.7 ± 32.2) ng/L,respectively,significantly higher than that of healthy controls [(0.57±0.14)％,t=15.72,P＜0.01; (64.2±20.7) ng/L,t=ll.31,P＜0.01].Patients with thyroid carcinomas had higher Th22 cells and IL-22 levels than that of patients with thyroid adenomes [(2.21±0.34)％ vs.(1.39±0.22)％,t=9.14,P＜0.01; (199.1 ±34.1) ng/Lvs.(129.8±31.3) ng/L,t =6.79,P ＜0.01].Compared with normal tissues,tumor tissues showed higher expression of CD4 + IL-22 + cells (56.25 ％ vs.8.33％,x2 =8.167,P ＜ 0.01).Compared with adenome tissues,carcinoma tissues showed higher expression of CD4 + IL-22 + cells (78.6％ vs.38.9％,x2 =5.039,P ＜0.05).The levels of Th22 cells in peripheral blood,IL-22 in serum and Th22 cells in tumor tissues was associated with tumor stage and lymph node metastasis (P ＜ 0.05).Conclusion There are higher quantification of Th22 cells and IL-22 in the peripheral blood and tumor tissues in patients with thyroid tumor and they have positive correlation with the clinical stage,Th22 cells and IL-22 may contribute to the disease progression and prognosis of thyroid tumor.
