中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2015年
1期
154-156
,共3页
石琴芳%冯梦宇%罗琨%杨娟%张波
石琴芳%馮夢宇%囉琨%楊娟%張波
석금방%풍몽우%라곤%양연%장파
乳腺癌%Ras相关区域家族1A基因%启动子%甲基化
乳腺癌%Ras相關區域傢族1A基因%啟動子%甲基化
유선암%Ras상관구역가족1A기인%계동자%갑기화
Breast cancer%Ras association domain family 1A gene%Promoter%Hypermethylation
目的 探讨Ras相关区域家族1A基因(RASSF1 A)启动子甲基化在乳腺癌发生发展中的作用.方法 甲基化特异性聚合酶链反应(MS-PCR)法分析44例乳腺癌石蜡包埋切片组织中RASSF 1A基因启动子甲基化状况,分析甲基化与临床病理特征之间的关系.结果 44例乳腺癌组织切片中有25例RASSF1A基因启动子发生了超甲基化,甲基化率为56.8%,启动子甲基化与患者年龄明显相关(P<0.05),与患者月经初潮年龄和生育次数无相关,与肿瘤大小、淋巴结转移、TNM分期及雌激素受体(ER)、孕激素受体(PR)之间无相关.结论 RASSF1A启动子甲基化为乳腺癌中的频发事件,其发生率随着年龄增长而升高,在乳腺癌发生中起重要作用.
目的 探討Ras相關區域傢族1A基因(RASSF1 A)啟動子甲基化在乳腺癌髮生髮展中的作用.方法 甲基化特異性聚閤酶鏈反應(MS-PCR)法分析44例乳腺癌石蠟包埋切片組織中RASSF 1A基因啟動子甲基化狀況,分析甲基化與臨床病理特徵之間的關繫.結果 44例乳腺癌組織切片中有25例RASSF1A基因啟動子髮生瞭超甲基化,甲基化率為56.8%,啟動子甲基化與患者年齡明顯相關(P<0.05),與患者月經初潮年齡和生育次數無相關,與腫瘤大小、淋巴結轉移、TNM分期及雌激素受體(ER)、孕激素受體(PR)之間無相關.結論 RASSF1A啟動子甲基化為乳腺癌中的頻髮事件,其髮生率隨著年齡增長而升高,在乳腺癌髮生中起重要作用.
목적 탐토Ras상관구역가족1A기인(RASSF1 A)계동자갑기화재유선암발생발전중적작용.방법 갑기화특이성취합매련반응(MS-PCR)법분석44례유선암석사포매절편조직중RASSF 1A기인계동자갑기화상황,분석갑기화여림상병리특정지간적관계.결과 44례유선암조직절편중유25례RASSF1A기인계동자발생료초갑기화,갑기화솔위56.8%,계동자갑기화여환자년령명현상관(P<0.05),여환자월경초조년령화생육차수무상관,여종류대소、림파결전이、TNM분기급자격소수체(ER)、잉격소수체(PR)지간무상관.결론 RASSF1A계동자갑기화위유선암중적빈발사건,기발생솔수착년령증장이승고,재유선암발생중기중요작용.
Objective To investigate the role of ras association domain family 1A (RASSF1A) promoter hypermethylation in the occurrence and development of breast cancer.Methods We analyzed the methylation status of RASSF1A promoter by methylation specific polymerase chain reaction (MS-PCR) of 44 formalin-fixed paraffin-embedded slides of sporadic primary breast cancer,collected corresponding clinicopathological data,and analyzed the relationship between methylation status and clinicopathological data.Results We found 25 out of 44 cases of breast cancer had hypermethylation with the occurrence rate being 56.8%.In this study,we found that methylation status of RASSF1A promoter was significantly associated with age of patients with breast cancer (P < 0.05),but not with menarche age and number of births.There was also no significant correlation between RASSF1A promoter methylation and tumor size,lymph node metastasis,TNM stage and estrogen receptor (ER),progesterone receptor (PR) manifestation status.Conclusion Our findings demonstrated that RASSF1A promoter methylation was a frequent event in breast cancer and plays an important role in the cancerogenesis.