CAJ | 학술논문

目的探讨胃腺癌组织中调节性T细胞(Tregs)浸润及其分布与胃腺癌患者预后的关系.方法免疫组织化学链霉菌抗生物素蛋白-过氧化物酶(SP)法检测106例胃腺癌组织中叉头样转录因子3(Foxp3)表达,结合随访资料分析胃癌组织中Tregs浸润数量及分布与患者预后的关系.结果癌组织中浸润的Tregs数量[(7.36±8.06)个]明显高于癌旁黏膜浸润的Tregs数量[(2.10±2.97)个,P＜0.01],癌间质中浸润的Tregs数量[(8.01±10.01)个]明显高于癌巢中浸润的Tregs数量[(0.47±1.49)个,P＜0.01];癌组织中浸润的Tregs与总生存时间(OS)无明显相关(P＞0.05);癌组织中癌巢浸润Tregs与OS呈正相关(P＜0.01).结论胃癌组织中存在Tregs浸润且分布具有差异,检测癌巢内浸润Tregs的水平有助于判断胃癌良好预后.
목적 탐토위선암조직중조절성T세포(Tregs)침윤급기분포여위선암환자예후적관계.방법 면역조직화학련매균항생물소단백-과양화물매(SP)법검측106례위선암조직중차두양전록인자3(Foxp3)표체,결합수방자료분석위암조직중Tregs침윤수량급분포여환자예후적관계.결과 암조직중침윤적Tregs수량[(7.36±8.06)개]명현고우암방점막침윤적Tregs수량[(2.10±2.97)개,P＜0.01],암간질중침윤적Tregs수량[(8.01±10.01)개]명현고우암소중침윤적Tregs수량[(0.47±1.49)개,P＜0.01];암조직중침윤적Tregs여총생존시간(OS)무명현상관(P＞0.05);암조직중암소침윤Tregs여OS정정상관(P＜0.01).결론 위암조직중존재Tregs침윤차분포구유차이,검측암소내침윤Tregs적수평유조우판단위암량호예후.
Objective To detect the infiltration of regulatory T cells (Tregs) in gastric cancer and evaluate its correlation with prognostic significance of gastric cancer.Methods The distribution of tumor-infiltrating Tregs and the expression of forkhead box protein 3 (Foxp3)-positive cancer cells were detected by immunohistochemistry staining technique to evaluate the prognostic effects in 106 cases of gastric adenocarcinoma.Results The average number of intratumoral Tregs (7.36 ± 8.06) was significantly greater than that in paired normal mucosa tissues (2.10 ± 2.97,P ＜ 0.01).The average number of Tregs in cancer stroma (8.01 ± 10.01) was significantly greater than that in paired cancer nests (0.47 ± 1.49,P ＜0.01).The number of intratumoral Tregs was not corrected with overall survival (OS,P ＞ 0.05).The number of Tregs in cancer nest was positively correlated with OS (P ＜ 0.01).Conclusion There are differences in the distribution of infiltrated Tregs between cancer nest and cancer stroma in gastric cancer.The level of the Tregs infiltration in cancer nests can be regarded as a predictive indicator for the prognosis in gastric cancer.