中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2015年
1期
171-174
,共4页
王海英%陈恩乐%周琴%张筱骅
王海英%陳恩樂%週琴%張篠驊
왕해영%진은악%주금%장소화
结肠癌%脱噬作用%新辅助化疗%调控蛋白
結腸癌%脫噬作用%新輔助化療%調控蛋白
결장암%탈서작용%신보조화료%조공단백
Colon cancer%Apoptosis%Neoadjuvant chemotherapy%Regulatory protein
目的 观察新辅助化疗(NACT)对结肠癌细胞凋亡及相关调控蛋白表达的影响.方法 收集结肠癌患者114例,采用原位末端标记法和免疫组织化学方法分别观察经NACT的FOLFOX4方案治疗前后结肠癌细胞凋亡和癌组织中B细胞淋巴瘤/白血病-2相关X蛋白(bax)、B细胞淋巴瘤/白血病-2(bcl-2)、环氧合酶-2(COX-2)、p53蛋白的表达,并判定NACT的近期疗效.结果 NACT化疗后结肠癌细胞发生典型凋亡形态学变化,细胞凋亡系数(3.87％比1.17％)和凋亡阻性率(59.65％比23.68％)显著高于化疗前.经NACT治疗后患者结肠癌细胞bcl-2(54.39％比70.15％)、COX-2(28.07％比50.00％)和p53(45.61％比70.05％)的阳性表达率与化疗前比较均有不同程度降低,差异有统计学意义(P＜0.05或P＜0.01),而bax阳性表达率(22.81％比50.88％)显著增强,差异有统计学意义(P＜0.01).同时bcl-2/bax比值(3.08比1.07)也显著降低.NACT治疗组的总有效率(39.47％)显著高于对照组(1.75％),治疗后患者无严重药物不良反应.结论 结肠癌患者经NACT化疗后癌组织中bcl-2、COX-2和p53蛋白表达明显降低,而bax蛋白的表达显著增加,促进癌细胞凋亡,且NACT的近期疗效显著.
目的 觀察新輔助化療(NACT)對結腸癌細胞凋亡及相關調控蛋白錶達的影響.方法 收集結腸癌患者114例,採用原位末耑標記法和免疫組織化學方法分彆觀察經NACT的FOLFOX4方案治療前後結腸癌細胞凋亡和癌組織中B細胞淋巴瘤/白血病-2相關X蛋白(bax)、B細胞淋巴瘤/白血病-2(bcl-2)、環氧閤酶-2(COX-2)、p53蛋白的錶達,併判定NACT的近期療效.結果 NACT化療後結腸癌細胞髮生典型凋亡形態學變化,細胞凋亡繫數(3.87％比1.17％)和凋亡阻性率(59.65％比23.68％)顯著高于化療前.經NACT治療後患者結腸癌細胞bcl-2(54.39％比70.15％)、COX-2(28.07％比50.00％)和p53(45.61％比70.05％)的暘性錶達率與化療前比較均有不同程度降低,差異有統計學意義(P＜0.05或P＜0.01),而bax暘性錶達率(22.81％比50.88％)顯著增彊,差異有統計學意義(P＜0.01).同時bcl-2/bax比值(3.08比1.07)也顯著降低.NACT治療組的總有效率(39.47％)顯著高于對照組(1.75％),治療後患者無嚴重藥物不良反應.結論 結腸癌患者經NACT化療後癌組織中bcl-2、COX-2和p53蛋白錶達明顯降低,而bax蛋白的錶達顯著增加,促進癌細胞凋亡,且NACT的近期療效顯著.
목적 관찰신보조화료(NACT)대결장암세포조망급상관조공단백표체적영향.방법 수집결장암환자114례,채용원위말단표기법화면역조직화학방법분별관찰경NACT적FOLFOX4방안치료전후결장암세포조망화암조직중B세포림파류/백혈병-2상관X단백(bax)、B세포림파류/백혈병-2(bcl-2)、배양합매-2(COX-2)、p53단백적표체,병판정NACT적근기료효.결과 NACT화료후결장암세포발생전형조망형태학변화,세포조망계수(3.87％비1.17％)화조망조성솔(59.65％비23.68％)현저고우화료전.경NACT치료후환자결장암세포bcl-2(54.39％비70.15％)、COX-2(28.07％비50.00％)화p53(45.61％비70.05％)적양성표체솔여화료전비교균유불동정도강저,차이유통계학의의(P＜0.05혹P＜0.01),이bax양성표체솔(22.81％비50.88％)현저증강,차이유통계학의의(P＜0.01).동시bcl-2/bax비치(3.08비1.07)야현저강저.NACT치료조적총유효솔(39.47％)현저고우대조조(1.75％),치료후환자무엄중약물불량반응.결론 결장암환자경NACT화료후암조직중bcl-2、COX-2화p53단백표체명현강저,이bax단백적표체현저증가,촉진암세포조망,차NACT적근기료효현저.
Objective To explore the effect of neoadjuvant chemotherapy (NACT) on the apoptosis and the expression of relevant regulatory protein in colon cancer.Methods 114 patients with colon cancer collected from my hospital were enrolled in the study from March 2011 to June 2014.Before and after treatment with FOLFOX4,apoptosis was examined by TdT-mediated dUTP nick end labeling (TUNEL) method,and the expression of B-cell lymphoma/leukemia-2 (bcl-2) associated X protein (bax),bcl-2,cyclooxygenase-2 (COX-2),and p53 was detected in colon cancer tissues by immunohistochemistry.The clinical efficacy of NACT was evaluated in short term.Results The apoptosis index (3.87％ vs.1.17％) and the apoptosis positive rate (59.65％ vs.23.68％) after treatment with NACT were significantly increased as compared with those before treatment in colon cancer (P ＜ 0.01).The positive rate ofbcl-2 (54.39％ vs.70.15％),COX-2 (28.07％ vs.50.00％),and p53 (45.61％ vs.70.05％) expression was decreased significantly (P ＜ 0.01 or P ＜ 0.05),while bax (22.81％ vs.50.88％),and the ratio of bcl-2 and bax (3.08 vs.1.07) were increased significantly (P ＜ 0.01).The total effective rate of the NACT group was 39.47％,and 1.75％ of the control group with the difference being significant difference between the two groups (P ＜0.01).There was no obvious side effect during treatment with NACT.Conclusion The down-regulation of bcl-2,COX-2,and p53 expression and the up-regulation of bax expression were observed in colon cancer after treatment with NACT.NACTfacilitates to the apoptosis of cancerous cells,and significantly improves the clinical efficacy.
