CAJ | 학술논문

目的检测尘肺病患者外周血清中基质金属蛋白酶(MMP)9和19的表达水平,探讨其作为尘肺病相关生物指标的可行性.方法选择98例男性尘肺病患者(Ⅰ期49例,Ⅱ期36例,Ⅲ期13例),其中矽肺41例,煤工尘肺57例.98例男性健康体检者作为对照.空腹情况下采集3 ml外周静脉血,分离血清,用酶联免疫吸附法测定样本血清中MMP9和MMP19水平.结果矽肺组血清中MMP9和MMP19表达均低于对照组,差异有统计学意义(P＜0.05);煤工尘肺组血清中MMP9和MMP19表达均低于对照组,差异有统计学意义(P＜0.05);矽肺组血清中MMP19表达水平高于煤工尘肺组,差异有统计学意义(P＜0.05).接尘工龄≤7年患者血清中MMP19表达水平高于接尘工龄＞20年的患者,差异有统计学意义(P＜0.05).不同肺功能损伤患者血清中MMP9和MMP19表达,差异无统计学意义(P＞0.05).尘肺病组与健康对照组血清中MMP9和MMP19表达均呈明显正相关关系(r=0.235,P＜0.05);血清中MMP9表达与尘肺分期呈负相关关系(P＜0.05).结论尘肺病患者血清中MMP9和MMP19表达变化有作为尘肺病相关生物标志物的潜能.
목적 검측진폐병환자외주혈청중기질금속단백매(MMP)9화19적표체수평,탐토기작위진폐병상관생물지표적가행성.방법 선택98례남성진폐병환자(Ⅰ기49례,Ⅱ기36례,Ⅲ기13례),기중석폐41례,매공진폐57례.98례남성건강체검자작위대조.공복정황하채집3 ml외주정맥혈,분리혈청,용매련면역흡부법측정양본혈청중MMP9화MMP19수평.결과 석폐조혈청중MMP9화MMP19표체균저우대조조,차이유통계학의의(P＜0.05);매공진폐조혈청중MMP9화MMP19표체균저우대조조,차이유통계학의의(P＜0.05);석폐조혈청중MMP19표체수평고우매공진폐조,차이유통계학의의(P＜0.05).접진공령≤7년환자혈청중MMP19표체수평고우접진공령＞20년적환자,차이유통계학의의(P＜0.05).불동폐공능손상환자혈청중MMP9화MMP19표체,차이무통계학의의(P＞0.05).진폐병조여건강대조조혈청중MMP9화MMP19표체균정명현정상관관계(r=0.235,P＜0.05);혈청중MMP9표체여진폐분기정부상관관계(P＜0.05).결론 진폐병환자혈청중MMP9화MMP19표체변화유작위진폐병상관생물표지물적잠능.
Objective To measure peripheral serum levels of matrix metalloproteinase 9 (MMP9) and matrix metalloproteinase 19 (MMP19) in patients with pneumoconiosis,and to investigate their feasibility as potential biomarkers for pneumoconiosis.Methods Ninety-eight male patients with pneumoconiosis (49patients in phase Ⅰ,36 patients in phase Ⅱ,and 13 patients in phase Ⅲ) were enrolled as subjects,which included 41 patients with silicosis and 57 patients with coal workers' pneumoconiosis.Ninety-eight healthy male physical examinees were used as controls.A fasting blood sample (3 ml) was collected from the peripheral venous blood of each patient or control,and the serum was separated from the blood sample.The expression levels of MMP9 and MMP19 in serum were measured by enzyme-linked immunosorbent assay.Results Serum levels of MMP9 and MMP19 in patients with silicosis or coal workers' pneumoconiosis were significantly lower than those in the control group (P＜0.05).Serum levels of MMP19 in patients with silicosis were significantly higher than those in patients with coal workers' pneumoconiosis (P＜0.05).Serum levels of MMP19 in patients exposed to dust for less than 7 years were significantly higher than those in patients exposed to dust for more than 20 years (P＜0.05).There were no significant differences in serum levels of MMP9 and MMP19 between patients with different levels of pulmonary function impairment (P＞0.05).Serum expression levels of MMP9 and MMP19 were positively correlated with each other in both patients with pneumoconiosis and those in the control group (P＜0.05).The serum expression level of MMP9 was negatively correlated with the stage of pneumoconiosis (P＜0.05).Conclusion Serum MMP9 and MMP19 may be used as potential biomarkers for pneumoconiosis.