中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2015年
1期
54-56,封4
,共4页
胡军%朱琳%杨少娟%许腾%高啸%周军
鬍軍%硃琳%楊少娟%許騰%高嘯%週軍
호군%주림%양소연%허등%고소%주군
硫化氢%糖尿病%胰岛素抵抗%脂代谢
硫化氫%糖尿病%胰島素牴抗%脂代謝
류화경%당뇨병%이도소저항%지대사
Hydrogen sulfide%Diabetes mellitus%Insulin resistance%Lipid metabolism
目的 观察硫化氢对2型糖尿病(T2DM)大鼠胰岛素抵抗和脂代谢的影响.方法 65只大鼠采用高糖高脂饮食+腹腔注射链脲佐菌素(STZ)的方法建立T2DM模型,将45只成模大鼠随机分为糖尿病(DM)、DM+硫氢化钠(NaHS)和DM+ DL-炔丙基甘氨酸(PAG)组,每组各15只,另设正常对照组.DM+ NaHS组大鼠腹腔注射NaHS[56 μmol/(kg·d)],DM+ PAG组腹腔注射PAG[50 mg/(kg·d)],对照组和DM组给予相同体积的生理盐水,连续给药2周.给药结束后,测定大鼠空腹血糖(FBG)、胰岛素(FIns)、甘油三酯(TG)、总胆固醇(TC)、游离脂肪酸(FFA),计算胰岛素抵抗指数(HOMA-IR)、胰岛素敏感指数(ISI);测定胰腺组织中硫化氢(H2S)浓度,免疫组织化学方法检测胰腺组织胰岛素表达.结果 与对照组比较,DM组大鼠FBG[(18.22±3.99) mmol/L]、TG[(1.54 ±0.16) mmol/L]、TC[(3.27±0.38) mmol/L]、FFA[(504.68±37.70) μmol/L]水平及HOMA-IR升高(P＜0.05),FIns[(41.79±3.43) mU/L]和ISI(-6.57 ±0.37)降低,胰腺组织H2S浓度[(96.98±19.44) μmol/L]升高,胰腺组织胰岛素阳性表达细胞面积(AIEP)和胰岛素阳性染色率(PRSI)降低,差异均有统计学意义(P＜0.05);与糖尿病组比较,NaHS干预后,糖尿病大鼠FBG[(25.42 ±0.21) mmol/L]、TG[(2.40 ±0.21) mmol/L]、TC[(4.80 ±0.16) mmol/L]、FFA[(633.96±25.64)μmol/L]水平及HOMA-IR升高更明显,FIns[(29.36±2.65) mU/L]和ISI(-6.58±0.27)降低,胰腺组织H2S浓度[(134.50±12.70)μmol/L]明显升高,AIEP和PRSI降低,差异均有统计学意义(P＜0.05);应用PAG后,糖尿病大鼠血浆FBG[(10.83±1.10) mmol/L]、TG[(1.30±0.12) mmol/L]、TC[(2.79 ±0.33) mmol/L]、FFA[(383.39±69.00) μmol/L]水平明显降低,胰岛素抵抗改善,FIns[(51.58±1.49) mU/L]和ISI(-6.32±0.11)明显升高,胰腺组织H2S浓度[(71.48±10.94) μmol/L]明显降低,AIEP和PRSI增加.结论 H2S通过影响T2DM大鼠胰岛素抵抗和脂代谢参与糖尿病发生发展.
目的 觀察硫化氫對2型糖尿病(T2DM)大鼠胰島素牴抗和脂代謝的影響.方法 65隻大鼠採用高糖高脂飲食+腹腔註射鏈脲佐菌素(STZ)的方法建立T2DM模型,將45隻成模大鼠隨機分為糖尿病(DM)、DM+硫氫化鈉(NaHS)和DM+ DL-炔丙基甘氨痠(PAG)組,每組各15隻,另設正常對照組.DM+ NaHS組大鼠腹腔註射NaHS[56 μmol/(kg·d)],DM+ PAG組腹腔註射PAG[50 mg/(kg·d)],對照組和DM組給予相同體積的生理鹽水,連續給藥2週.給藥結束後,測定大鼠空腹血糖(FBG)、胰島素(FIns)、甘油三酯(TG)、總膽固醇(TC)、遊離脂肪痠(FFA),計算胰島素牴抗指數(HOMA-IR)、胰島素敏感指數(ISI);測定胰腺組織中硫化氫(H2S)濃度,免疫組織化學方法檢測胰腺組織胰島素錶達.結果 與對照組比較,DM組大鼠FBG[(18.22±3.99) mmol/L]、TG[(1.54 ±0.16) mmol/L]、TC[(3.27±0.38) mmol/L]、FFA[(504.68±37.70) μmol/L]水平及HOMA-IR升高(P＜0.05),FIns[(41.79±3.43) mU/L]和ISI(-6.57 ±0.37)降低,胰腺組織H2S濃度[(96.98±19.44) μmol/L]升高,胰腺組織胰島素暘性錶達細胞麵積(AIEP)和胰島素暘性染色率(PRSI)降低,差異均有統計學意義(P＜0.05);與糖尿病組比較,NaHS榦預後,糖尿病大鼠FBG[(25.42 ±0.21) mmol/L]、TG[(2.40 ±0.21) mmol/L]、TC[(4.80 ±0.16) mmol/L]、FFA[(633.96±25.64)μmol/L]水平及HOMA-IR升高更明顯,FIns[(29.36±2.65) mU/L]和ISI(-6.58±0.27)降低,胰腺組織H2S濃度[(134.50±12.70)μmol/L]明顯升高,AIEP和PRSI降低,差異均有統計學意義(P＜0.05);應用PAG後,糖尿病大鼠血漿FBG[(10.83±1.10) mmol/L]、TG[(1.30±0.12) mmol/L]、TC[(2.79 ±0.33) mmol/L]、FFA[(383.39±69.00) μmol/L]水平明顯降低,胰島素牴抗改善,FIns[(51.58±1.49) mU/L]和ISI(-6.32±0.11)明顯升高,胰腺組織H2S濃度[(71.48±10.94) μmol/L]明顯降低,AIEP和PRSI增加.結論 H2S通過影響T2DM大鼠胰島素牴抗和脂代謝參與糖尿病髮生髮展.
목적 관찰류화경대2형당뇨병(T2DM)대서이도소저항화지대사적영향.방법 65지대서채용고당고지음식+복강주사련뇨좌균소(STZ)적방법건립T2DM모형,장45지성모대서수궤분위당뇨병(DM)、DM+류경화납(NaHS)화DM+ DL-결병기감안산(PAG)조,매조각15지,령설정상대조조.DM+ NaHS조대서복강주사NaHS[56 μmol/(kg·d)],DM+ PAG조복강주사PAG[50 mg/(kg·d)],대조조화DM조급여상동체적적생리염수,련속급약2주.급약결속후,측정대서공복혈당(FBG)、이도소(FIns)、감유삼지(TG)、총담고순(TC)、유리지방산(FFA),계산이도소저항지수(HOMA-IR)、이도소민감지수(ISI);측정이선조직중류화경(H2S)농도,면역조직화학방법검측이선조직이도소표체.결과 여대조조비교,DM조대서FBG[(18.22±3.99) mmol/L]、TG[(1.54 ±0.16) mmol/L]、TC[(3.27±0.38) mmol/L]、FFA[(504.68±37.70) μmol/L]수평급HOMA-IR승고(P＜0.05),FIns[(41.79±3.43) mU/L]화ISI(-6.57 ±0.37)강저,이선조직H2S농도[(96.98±19.44) μmol/L]승고,이선조직이도소양성표체세포면적(AIEP)화이도소양성염색솔(PRSI)강저,차이균유통계학의의(P＜0.05);여당뇨병조비교,NaHS간예후,당뇨병대서FBG[(25.42 ±0.21) mmol/L]、TG[(2.40 ±0.21) mmol/L]、TC[(4.80 ±0.16) mmol/L]、FFA[(633.96±25.64)μmol/L]수평급HOMA-IR승고경명현,FIns[(29.36±2.65) mU/L]화ISI(-6.58±0.27)강저,이선조직H2S농도[(134.50±12.70)μmol/L]명현승고,AIEP화PRSI강저,차이균유통계학의의(P＜0.05);응용PAG후,당뇨병대서혈장FBG[(10.83±1.10) mmol/L]、TG[(1.30±0.12) mmol/L]、TC[(2.79 ±0.33) mmol/L]、FFA[(383.39±69.00) μmol/L]수평명현강저,이도소저항개선,FIns[(51.58±1.49) mU/L]화ISI(-6.32±0.11)명현승고,이선조직H2S농도[(71.48±10.94) μmol/L]명현강저,AIEP화PRSI증가.결론 H2S통과영향T2DM대서이도소저항화지대사삼여당뇨병발생발전.
Objective To investigate the effects of hydrogen sulfide on insulin sensitivity and lipid metabolism in type 2 diabetic rat model.Methods The type 2 diabetes mellitus (T2DM) rat model was established by high-fat and high-glucose diet and low-dose intraperitoneal injection of stretopzin (STZ).The model rats were randomly divided into diabetes(DM) group (n =15),DM + NaHS group (n =15),and DM + DL-propargylglycine(PAG) group (n =15),and the normal control group was set up (n =15).The animals in DM + NaHS group were given NaHS [56 μmol/(kg· d)],those in DM +PAG group given PAG [50 mg/(kg·d)],and those in control and DM groups given the same volume of saline for 2 weeks.Behavioral characteristics were observed in all groups.The serum levels of fasting blood glucose (FBG) and insulin (FIns),triglycerides (TG),total cholesterol (TC),free fatty acid (FFA) and hydrogen sulfide (H2S) concentrations in the diabetic models were measured to calculate HOMA-IR and ISI.H2S concentrations in pancreatic tissue were measured.The expression of insulin was detected by immunohistochemistry in pancreatic tissue.Results The typical symptoms of diabetes were observed in T2DM rats.As compared with control group,plasma FBG [(18.22 ± 3.99) mmol/L],TG [(1.54 ±0.16) mmol/L],TC [(3.27 ± 0.38) mmol/L],FFA [(504.68 ± 37.70) μmol/L] levels and HOMA-IR were significantly increased in DM group; FIns [(41.79 ±3.43) mU/L] and ISI (-6.57 ±0.37) were significantly decreased,H2S concentration [(96.98 ± 19.44) μmol/L] in pancreatic tissue was significantly increased,and the areas of insulin-expression positive cells and positive rate of insulin staining cells were significantly decreased (P ＜ 0.05).As compared with DM group,plasma FBG [(25.42±0.21) mmol/L],TG [(2.40 ±0.21) mmol/L],TC [(4.80 ±0.16) mmol/L],FFA [(633.96 ±25.64) μmol/L] levels and HOMA-IR were significantly increased in DM + NaHS group; FIns [(29.36 ±2.65) mU/L] and ISI (-6.58 ±0.27) were significantly decreased,H2S concentration in pancreatic tissue [(134.50 ± 12.70) μmol/L] was significantly increased,and the areas of insulin-expression positive cells and positive rate of insulin staining cells were significantly decreased (P ＜ 0.05).As compared with DM group,plasma FBG [(10.83 ± 1.10) mmol/L],TG [(1.30 ±0.12) mmol/L],TC [(2.79 ±0.33) mmol/L],FFA [(383.39 ±69.00) μmol/L] levels and HOMA-IR were significantly decreased in DM + PAG group,FIns [(51.58 ± 1.49) mU/L] and ISI (-6.32 ± 0.11) were significantly increased,H2S concentration in pancreas tissue [(71.48 ± 10.94) μmol/L] was significantly decreased,and the areas of insulin-expression positive cells and positive rate of insulin staining cells were significantly increased (P ＜ 0.05).Conclusion H2S may be involved in the development of T2DM through influencing the insulin sensitivity and lipid metabolism.
