CAJ | 학술논문

目的观察p53正向细胞凋亡调控因子基因(PUMA)的表达对关节软骨细胞凋亡的影响.方法通过白细胞介素(IL)-1β干预建立小鼠骨关节炎模型,再采用小分子RNA干扰片段对小鼠软骨细胞中的PUMA进行抑制,转染前后分别检测PUMA蛋白的表达及细胞凋亡.结果空转染对照组PUMA蛋白表达量为空白对照组的6.11倍,空转染对照组分别为两个实验组的2.02倍和3.30倍.染色显示细胞凋亡率在空白对照组为3.6％,空转染对照组29.6％,两个实验组分别为9.0％和13.2％,实验组和空转染对照组的差异有统计学意义(P＜0.01),抑制PUMA表达后细胞凋亡明显减少.结论骨关节炎小鼠软骨细胞中PUMA基因表达增加,提示PUMA基因参与了骨关节炎软骨细胞凋亡.
목적 관찰p53정향세포조망조공인자기인(PUMA)적표체대관절연골세포조망적영향.방법 통과백세포개소(IL)-1β간예건립소서골관절염모형,재채용소분자RNA간우편단대소서연골세포중적PUMA진행억제,전염전후분별검측PUMA단백적표체급세포조망.결과 공전염대조조PUMA단백표체량위공백대조조적6.11배,공전염대조조분별위량개실험조적2.02배화3.30배.염색현시세포조망솔재공백대조조위3.6％,공전염대조조29.6％,량개실험조분별위9.0％화13.2％,실험조화공전염대조조적차이유통계학의의(P＜0.01),억제PUMA표체후세포조망명현감소.결론 골관절염소서연골세포중PUMA기인표체증가,제시PUMA기인삼여료골관절염연골세포조망.
Objective To explore the effects of p53 upregulated modulator of apoptosis (PUMA) on apoptosis in the mouse knee joint osteoarthritis cartilage.Methods The osteoarthritis cell model was made by using mouse artificial chondrocytes treated with interleukin (IL)-1β,and small interfering RNA (siRNA) of PUMA was transfected into chondrocytes.The expression of PUMA and the apoptosis were detected by Western blotting and Hoechst staining at baseline and after transfection.Results PUMA protein expression in control group was 6.11 times of blank group,and that in control group was 2.02 and 3.30 times of experimental groups.The apoptosis rate in blank group,control group,and experimental groups A and B was 3.6％,29.6％,9.0％ and 13.2％ respectively.SiRNA of PUMA had been transfected into chondrocytes successfully.PUMA protein expression was suppressed,and apoptosis of chondrocytes decreased.Conclusion PUMA gene has taken part in the apoptosis of chondrocytes in mice with osteoarthritis.