中华神经外科杂志
中華神經外科雜誌
중화신경외과잡지
Chinese Journal of Neurosurgery
2015年
1期
84-87
,共4页
张白%惠品晶%国风%马亮%吕琦%颜燕红%黄亚波%惠国桢
張白%惠品晶%國風%馬亮%呂琦%顏燕紅%黃亞波%惠國楨
장백%혜품정%국풍%마량%려기%안연홍%황아파%혜국정
颈动脉内膜剥脱术%动脉粥样硬化%斑块稳定性%抗原,CD40%基质金属蛋白酶
頸動脈內膜剝脫術%動脈粥樣硬化%斑塊穩定性%抗原,CD40%基質金屬蛋白酶
경동맥내막박탈술%동맥죽양경화%반괴은정성%항원,CD40%기질금속단백매
Carotid endarterectomy%Atherosclerosis%Plaque stability%Antigens,CD40%Matrix metalloproteinases
目的 研究CD40及基质金属蛋白酶(MMPs)在颈动脉易损斑块及稳定斑块中的表达,探讨CD40与MMPs表达的相关性及影响斑块稳定性的可能机制.方法 收集颈动脉内膜剥脱术(CEA)后的粥样硬化斑块标本32例次,根据术前颈动脉超声、经颅多普勒(TCD)及是否发生急性缺血性脑卒中事件分为易损斑块组及稳定斑块组,采用PCR和Western blot法检测两组标本中CD40及MMPs的mRNA和蛋白表达水平.结果 易损斑块组CD40和MMP-9 mRNA及蛋白表达水平均较稳定斑块组显著增高(P＜0.01);MMP-2蛋白表达水平亦较稳定斑块组明显增高(P ＜0.01);CD40mRNA与MMP-2 mRNA(r=0.43)、CD40 mRNA与MMP-9 mRNA(r=0.90)之间呈正相关(P＜0.05).结论 CD40不但参与动脉粥样硬化斑块的形成,而且可能通过调控MMP-2、MMP-9的表达影响动脉粥样硬化斑块的稳定性.
目的 研究CD40及基質金屬蛋白酶(MMPs)在頸動脈易損斑塊及穩定斑塊中的錶達,探討CD40與MMPs錶達的相關性及影響斑塊穩定性的可能機製.方法 收集頸動脈內膜剝脫術(CEA)後的粥樣硬化斑塊標本32例次,根據術前頸動脈超聲、經顱多普勒(TCD)及是否髮生急性缺血性腦卒中事件分為易損斑塊組及穩定斑塊組,採用PCR和Western blot法檢測兩組標本中CD40及MMPs的mRNA和蛋白錶達水平.結果 易損斑塊組CD40和MMP-9 mRNA及蛋白錶達水平均較穩定斑塊組顯著增高(P＜0.01);MMP-2蛋白錶達水平亦較穩定斑塊組明顯增高(P ＜0.01);CD40mRNA與MMP-2 mRNA(r=0.43)、CD40 mRNA與MMP-9 mRNA(r=0.90)之間呈正相關(P＜0.05).結論 CD40不但參與動脈粥樣硬化斑塊的形成,而且可能通過調控MMP-2、MMP-9的錶達影響動脈粥樣硬化斑塊的穩定性.
목적 연구CD40급기질금속단백매(MMPs)재경동맥역손반괴급은정반괴중적표체,탐토CD40여MMPs표체적상관성급영향반괴은정성적가능궤제.방법 수집경동맥내막박탈술(CEA)후적죽양경화반괴표본32례차,근거술전경동맥초성、경로다보륵(TCD)급시부발생급성결혈성뇌졸중사건분위역손반괴조급은정반괴조,채용PCR화Western blot법검측량조표본중CD40급MMPs적mRNA화단백표체수평.결과 역손반괴조CD40화MMP-9 mRNA급단백표체수평균교은정반괴조현저증고(P＜0.01);MMP-2단백표체수평역교은정반괴조명현증고(P ＜0.01);CD40mRNA여MMP-2 mRNA(r=0.43)、CD40 mRNA여MMP-9 mRNA(r=0.90)지간정정상관(P＜0.05).결론 CD40불단삼여동맥죽양경화반괴적형성,이차가능통과조공MMP-2、MMP-9적표체영향동맥죽양경화반괴적은정성.
Objective To study the expression level of CD40 and matrix metalloproteinase (MMPs) in vulnerability,stability carotid atherosclerotic plaque.Investigate the relevance of CD40 and MMPs expression and the impact on plaque stability.Methods Thirty-two cases of specimens with carotid atherosclerotic plaque were collected from carotid endarterectomy (CEA).According to preoperative carotid ultrasound,transcranial doppler (TCD) and the presence of acute ischemic stroke events,all specimens were divided into stable plaque group and vulnerable plaque group,detected the mRNA and protein expression level of CD40 and MMPs in each group using PCR and western blotting assay.Results The mRNA and protein expression level of CD40 and MMP-9 in vulnerable plaque group was significantly higher than in stable plaque group respectively (P ＜ 0.01).The MMP-2 protein expression level in vulnerable plaque group was distinctly higher than in stable plaque group(P ＜0.01).There were positive correlation between CD40 mRNA and MMP-2 mRNA (r =0.43,P ＜ 0.05),CD40 mRNA and MMP-9 mRNA (r =0.90,P ＜0.01) respectively.Conclusion CD40 is not only involved in the formation of atherosclerotic plaque,but also may influence the stability of atherosclerotic plaques by regulating the expression of MMP-2 and MMP-9.
