CAJ | 학술논문

目的探讨CD157蛋白在人胃癌组织中的表达情况及其与胃癌患者临床病理指标和预后的关系;初步探讨CD157对胃癌AGS细胞侵袭转移的作用及其机制.方法用免疫组织化学方法检测CD157蛋白在90例胃癌及配对癌旁组织中的表达,分析其表达结果与胃癌患者的临床病理资料和预后的相关性.通过小干扰RNA(small interfering RNA,siRNA)转染人胃癌AGS细胞下调CD157的表达,用Transwell侵袭实验检测CD157对胃癌AGS细胞侵袭能力的影响.结果 CD157蛋白在胃癌组织及癌旁组织的表达率分别为67％和12％,差异有统计学意义(x2=55.84,P＜0.01);CD157蛋白的表达水平与肿瘤浸润深度(x2=12.503,P＜0.01)、淋巴结转移(x2=8.693,P=0.003)和远处转移(x2=4.944,P=0.027)相关,而与年龄(x2=1.659,P=0.198)、性别(x2=1.431,P=0.232)和分化程度(x2 =0.407,P=0.856)无相关;CD157高表达组胃癌患者中位生存期短于低表达组(29.2个月比46.0个月,x2=4.438,P=0.036).与对照组相比,siRNA组CD157 RNA表达(t=45.004,P＜0.01)和蛋白表达(t=32.877,P＜0.01)降低,侵袭能力减弱(F=98.455,P＜0.01).结论 CD157高表达与胃癌的浸润、转移和不良预后有关,下调CD157表达可抑制胃癌细胞侵袭能力.
목적 탐토CD157단백재인위암조직중적표체정황급기여위암환자림상병리지표화예후적관계;초보탐토CD157대위암AGS세포침습전이적작용급기궤제.방법 용면역조직화학방법검측CD157단백재90례위암급배대암방조직중적표체,분석기표체결과여위암환자적림상병리자료화예후적상관성.통과소간우RNA(small interfering RNA,siRNA)전염인위암AGS세포하조CD157적표체,용Transwell침습실험검측CD157대위암AGS세포침습능력적영향.결과 CD157단백재위암조직급암방조직적표체솔분별위67％화12％,차이유통계학의의(x2=55.84,P＜0.01);CD157단백적표체수평여종류침윤심도(x2=12.503,P＜0.01)、림파결전이(x2=8.693,P=0.003)화원처전이(x2=4.944,P=0.027)상관,이여년령(x2=1.659,P=0.198)、성별(x2=1.431,P=0.232)화분화정도(x2 =0.407,P=0.856)무상관;CD157고표체조위암환자중위생존기단우저표체조(29.2개월비46.0개월,x2=4.438,P=0.036).여대조조상비,siRNA조CD157 RNA표체(t=45.004,P＜0.01)화단백표체(t=32.877,P＜0.01)강저,침습능력감약(F=98.455,P＜0.01).결론 CD157고표체여위암적침윤、전이화불량예후유관,하조CD157표체가억제위암세포침습능력.
Objective To investigate the expression of CD157 in gastric cancer and its relationship with clinicopathological parameters and prognosis,and to explore the effects and molecular mechanisms of CD157 in invasion and metastasis of gastric cancer cells.Method The expression of CD157 in 90 cases of gastric cancer tissues and corresponding adjacent non-cancerous tissues (ANCT) was detected using immunohistochemistry.The correlation between the expression of CD157 and clinicopathological parameters and prognosis was analyzed statistically.Human gastric cancer AGS cells in vitro were transfected with small interfering RNA to down regulate the expression of CD157.The invasion capability of gastric cancer cells were assessed by Transwell assays.Result High expression of CD157 protein was observed in 67％ gastric adenocarcinoma compared with that in 12％ ANCT (x2 =55.84,P ＜0.01).The expression of CD157 was correlated with the depth of tumor infiltration (x2 =12.503,P ＜ 0.01),lymph node metastasis (x2 =8.693,P =0.003) and distant metastasis (x2 =8.693,P =0.003),but not with age (x2 =1.659,P =0.198),gender (x2 =1.43 1,P =0.232) or tumor differentiation (x2 =0.407,P =0.856).Patients with high expression of CD157 had shorter survival (29.0 ± 16.7 months) than those with low expression(46.0 ± 18.2 months) (x2 =4.438,P =0.036).AGS cells interfered by siRNA has lower expression of CD157 both in protein (t =32.877,P ＜ 0.01) and RNA (t =45.004,P ＜ 0.01) levels.Down regulated expression of CD157 could inhibit invasion potential of gastric cancer cells (F =98.455,P ＜ 0.01).Conclusions High expression of CD157 might play an important role in the poor survival of gastric cancer.Down-regulation of CD157 can reduce invasive ability of gastric cancer cells.