中国医药
中國醫藥
중국의약
CHINA MEDICINE
2015年
2期
153-157
,共5页
代青湘%许慧宁%李闯兵%韩吉祥%徐娟%朱集兰%李英莲%常琼%刘军莉
代青湘%許慧寧%李闖兵%韓吉祥%徐娟%硃集蘭%李英蓮%常瓊%劉軍莉
대청상%허혜저%리틈병%한길상%서연%주집란%리영련%상경%류군리
亚甲基四氢叶酸还原酶%多态性,单核苷酸%动脉顺应性
亞甲基四氫葉痠還原酶%多態性,單覈苷痠%動脈順應性
아갑기사경협산환원매%다태성,단핵감산%동맥순응성
Methylenetetrahydrofolate reductase C677T%Polymorphisim,Single nudeotide%Arterial compliance
目的 探讨亚甲基四氢叶酸还原酶(MTHFR) C677T基因多态性与青海西宁地区汉族人群原发性高血压及动脉顺应性的相关性.方法 对2012年8月至2014年7月于青海大学附属医院114例原发性高血压患者(原发性高血压组)和104例年龄匹配的正常对照者(正常对照组),采用聚合酶链反应和限制性片段长度多态性分析技术进行基因多态性分析,采用3％琼脂凝胶电泳判断基因型及测序,并测定肱-踝动脉脉搏波速度(baPWV)、踝臂指数.结果 原发性高血压组TT基因型及T等位基因频率明显高于正常对照组[28.1％ (32/114)和75.4％ (86/114)比25.0％ (26/104)和64.4％ (67/104)],差异有统计学意义(P=0.018和0.002);原发性高血压组血氧饱和度明显低于正常对照组[(90±3)％比(92±2)％,P ＜0.05].CC基因型中原发性高血压组baPWV明显高于正常对照组[(16.2±3.8) m/s比(12.8±1.7) m/s],踝臂指数明显高于正常对照组(1.21 ±0.13比1.10 ±0.13),差异均有统计学意义(均P＜0.05);TT+TC基因型组中原发性高血压组baPWV明显高于正常对照组[(16.9±3.0) m/s比(12.9±3.0)m/s],踝臂指数明显高于正常对照组(1.27 ±0.18比1.16±0.12),差异均有统计学意义(均P＜0.05).原发性高血压组TT+TC基因型baPWV和踝臂指数明显高于CC基因型[(16.9 ±3.0)m/s比(16.2±3.8)m/s,1.27 ±0.18比1.21 ±0.13,P＜0.05];正常对照组TT+TC基因型踝臂指数明显高于CC基因型(1.16 ±0.12比1.10 ±0.13),差异有统计学意义(P＜0.05).结论 MTHFR C677T多态性与青海西宁地区原发性高血压发病有关,MTHFRC677T等位基因可能是高血压动脉硬化的遗传因素,而在高原低氧因素下可能进一步增加发病率.
目的 探討亞甲基四氫葉痠還原酶(MTHFR) C677T基因多態性與青海西寧地區漢族人群原髮性高血壓及動脈順應性的相關性.方法 對2012年8月至2014年7月于青海大學附屬醫院114例原髮性高血壓患者(原髮性高血壓組)和104例年齡匹配的正常對照者(正常對照組),採用聚閤酶鏈反應和限製性片段長度多態性分析技術進行基因多態性分析,採用3％瓊脂凝膠電泳判斷基因型及測序,併測定肱-踝動脈脈搏波速度(baPWV)、踝臂指數.結果 原髮性高血壓組TT基因型及T等位基因頻率明顯高于正常對照組[28.1％ (32/114)和75.4％ (86/114)比25.0％ (26/104)和64.4％ (67/104)],差異有統計學意義(P=0.018和0.002);原髮性高血壓組血氧飽和度明顯低于正常對照組[(90±3)％比(92±2)％,P ＜0.05].CC基因型中原髮性高血壓組baPWV明顯高于正常對照組[(16.2±3.8) m/s比(12.8±1.7) m/s],踝臂指數明顯高于正常對照組(1.21 ±0.13比1.10 ±0.13),差異均有統計學意義(均P＜0.05);TT+TC基因型組中原髮性高血壓組baPWV明顯高于正常對照組[(16.9±3.0) m/s比(12.9±3.0)m/s],踝臂指數明顯高于正常對照組(1.27 ±0.18比1.16±0.12),差異均有統計學意義(均P＜0.05).原髮性高血壓組TT+TC基因型baPWV和踝臂指數明顯高于CC基因型[(16.9 ±3.0)m/s比(16.2±3.8)m/s,1.27 ±0.18比1.21 ±0.13,P＜0.05];正常對照組TT+TC基因型踝臂指數明顯高于CC基因型(1.16 ±0.12比1.10 ±0.13),差異有統計學意義(P＜0.05).結論 MTHFR C677T多態性與青海西寧地區原髮性高血壓髮病有關,MTHFRC677T等位基因可能是高血壓動脈硬化的遺傳因素,而在高原低氧因素下可能進一步增加髮病率.
목적 탐토아갑기사경협산환원매(MTHFR) C677T기인다태성여청해서저지구한족인군원발성고혈압급동맥순응성적상관성.방법 대2012년8월지2014년7월우청해대학부속의원114례원발성고혈압환자(원발성고혈압조)화104례년령필배적정상대조자(정상대조조),채용취합매련반응화한제성편단장도다태성분석기술진행기인다태성분석,채용3％경지응효전영판단기인형급측서,병측정굉-과동맥맥박파속도(baPWV)、과비지수.결과 원발성고혈압조TT기인형급T등위기인빈솔명현고우정상대조조[28.1％ (32/114)화75.4％ (86/114)비25.0％ (26/104)화64.4％ (67/104)],차이유통계학의의(P=0.018화0.002);원발성고혈압조혈양포화도명현저우정상대조조[(90±3)％비(92±2)％,P ＜0.05].CC기인형중원발성고혈압조baPWV명현고우정상대조조[(16.2±3.8) m/s비(12.8±1.7) m/s],과비지수명현고우정상대조조(1.21 ±0.13비1.10 ±0.13),차이균유통계학의의(균P＜0.05);TT+TC기인형조중원발성고혈압조baPWV명현고우정상대조조[(16.9±3.0) m/s비(12.9±3.0)m/s],과비지수명현고우정상대조조(1.27 ±0.18비1.16±0.12),차이균유통계학의의(균P＜0.05).원발성고혈압조TT+TC기인형baPWV화과비지수명현고우CC기인형[(16.9 ±3.0)m/s비(16.2±3.8)m/s,1.27 ±0.18비1.21 ±0.13,P＜0.05];정상대조조TT+TC기인형과비지수명현고우CC기인형(1.16 ±0.12비1.10 ±0.13),차이유통계학의의(P＜0.05).결론 MTHFR C677T다태성여청해서저지구원발성고혈압발병유관,MTHFRC677T등위기인가능시고혈압동맥경화적유전인소,이재고원저양인소하가능진일보증가발병솔.
Objective To study the relation of Methylenetetrahydrofolate reductase (MTHFR) gene C677T polymorphism and essential hypertension (EH) among han population in xining,qinghai region.Methods The study was performed in 114 patients with essential hypertension and 104 age matched normal control in department of Cardiology and Geriatrics in the Affiliated Hospital of Qinghai University from August 2012 to July 2014.MTHFR gene C677T polymorphism was analyzed by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism(PCR-RFLP) and characterized by electrophoresis and sequencing,brachial-ankle arterial pulse wave velocity (Ba-PWY),ankle-brachial index (ABI) were also investigated.Results In essential hypertension group the frequencies of TT genotype and T allele were significantly higher than those of normal control group(28.1％ vs 25.0％ and 75.4％ vs 64.4％,P ＜0.05).Essential hypertension group oxygen saturation were Obviously lower than the control group [(90 ± 30％ vs (92±2) ％,P ＜0.05].Essential hypertension group the BaPWV higher than those of normal control group in the CC genotype [(16.2±3.8) m/s vs (12.8 ± 1.7) m/s,P ＜ 0.05],and the ABI was significantly higher than those of normal control group[(1.21 ±0.13) vs (1.10 ±0.13),P ＜0.05].In TT + TC genotype the essential hypertension group baPWV was significantly higher than those of normal control group,[(16.9 ± 3.0) m/s vs (12.9 ± 3.0) m/s,P ＜ 0.05],and ABI was significantly higher than those of normal control group [(1.27 ±0.18) vs (1.16 ± 0.12),P ＜ 0.05].In Essential hypertension group TT + TC genotype's baPWV was significantly higher than CC genotype's[(16.9 ±3.0) m/s vs (16.2±3.8) m/s,P ＜0.05],and the ABI was significantly higher than CC genotype's [(1.27 ± 0.18) vs (1.21 ± 0.13),P ＜ 0.05].In control group TT +TC genotype's ABI was significantly higher than CC genotype's [(1.16-±0.12)vs(1.10 ±0.13),P ＜0.05].Conclusion MTHFR C677T gene polymorphism is related to high risk of essential hypertension.
