中国医药
中國醫藥
중국의약
CHINA MEDICINE
2015年
1期
61-65
,共5页
张俊红%王文革%耿扬%毕淑英
張俊紅%王文革%耿颺%畢淑英
장준홍%왕문혁%경양%필숙영
支原体肺炎%CD4+CD25+Treg细胞%维生素A
支原體肺炎%CD4+CD25+Treg細胞%維生素A
지원체폐염%CD4+CD25+Treg세포%유생소A
Mycoplasma pneumoniae pneumonia%CD4+CD25+ regulatory T cells%Vitamin A
目的 探讨肺炎支原体肺炎(MPP)患儿外周血淋巴细胞中CD4+ CD25+调节性T细胞(Treg)、T细胞亚群CD3+、CD4+、CD4+/CDs水平的变化及临床意义;探讨体外实验时维生素A体内活性代谢产物视黄酸对MPP患儿外周血淋巴细胞CD4+ CD25+ Treg细胞、T细胞亚群CD3+、CD4+表达及CD4+/CD8+比率的调节干预作用.方法 选择2012年10-12月在空军总医院儿科确诊为MPP的20例住院患儿作为研究对象,其中10例患儿作为病例试验组,另10例患儿作为病例对照组(即未与视黄酸孵育),同期10例同龄健康儿童作为正常对照组.收集患儿及正常对照儿童外周血标本,分离单个核细胞,用流式细胞仪检测外周血淋巴细胞中CD4+ CD25+ Treg细胞、T细胞亚群CD3+、CD4+、CD4+/CD8+水平,病例试验组提取其淋巴细胞在体外培养基中加入一定量视黄酸共同培养孵育,再次测定CD4+ CD25+ Treg细胞、T细胞亚群CD3+、CD4+、CD4+/CD8+水平,将上述指标进行比较分析.结果 病例组患儿外周血淋巴细胞中CD4+ CD25+ Treg细胞、CD4+/CD8+、CD3+百分比分别为(4.5±1.9)％、(1.3±0.9)％、(38.9±11.4)％,正常对照组儿童分别为(13.2±2.5)％、(7.9±3.0)％、(65.1±8.8)％,病例对照组明显低于正常对照组,差异有统计学意义(P＜0.05),而与视黄酸共同孵育后病例试验组以上指标明显升高,分别上升至(9.2±3.8)％、(5.9±2.5)％、(81.3±11.6)％,与病例对照组比较差异有统计学意义(P＜0.05),与正常对照组比较差异无统计学意义(P＞0.05).结论 MPP患儿外周血淋巴细胞中CD4+ CD25+ Treg细胞、CD3+T细胞表达明显受抑,CD4+/CD8+亚群比例异常,T细胞亚群数量及功能明显紊乱,而体外试验时维生素A体内活性代谢产物视黄酸干预后能增强淋巴细胞CD4+ CD25+ Treg细胞、CD3+ T细胞表达,恢复CD4+/CD8+亚群比例,纠正T细胞亚群功能失衡,从而增强、协调机体免疫、抗感染能力,为维生素A辅助治疗MPP提高参考.
目的 探討肺炎支原體肺炎(MPP)患兒外週血淋巴細胞中CD4+ CD25+調節性T細胞(Treg)、T細胞亞群CD3+、CD4+、CD4+/CDs水平的變化及臨床意義;探討體外實驗時維生素A體內活性代謝產物視黃痠對MPP患兒外週血淋巴細胞CD4+ CD25+ Treg細胞、T細胞亞群CD3+、CD4+錶達及CD4+/CD8+比率的調節榦預作用.方法 選擇2012年10-12月在空軍總醫院兒科確診為MPP的20例住院患兒作為研究對象,其中10例患兒作為病例試驗組,另10例患兒作為病例對照組(即未與視黃痠孵育),同期10例同齡健康兒童作為正常對照組.收集患兒及正常對照兒童外週血標本,分離單箇覈細胞,用流式細胞儀檢測外週血淋巴細胞中CD4+ CD25+ Treg細胞、T細胞亞群CD3+、CD4+、CD4+/CD8+水平,病例試驗組提取其淋巴細胞在體外培養基中加入一定量視黃痠共同培養孵育,再次測定CD4+ CD25+ Treg細胞、T細胞亞群CD3+、CD4+、CD4+/CD8+水平,將上述指標進行比較分析.結果 病例組患兒外週血淋巴細胞中CD4+ CD25+ Treg細胞、CD4+/CD8+、CD3+百分比分彆為(4.5±1.9)％、(1.3±0.9)％、(38.9±11.4)％,正常對照組兒童分彆為(13.2±2.5)％、(7.9±3.0)％、(65.1±8.8)％,病例對照組明顯低于正常對照組,差異有統計學意義(P＜0.05),而與視黃痠共同孵育後病例試驗組以上指標明顯升高,分彆上升至(9.2±3.8)％、(5.9±2.5)％、(81.3±11.6)％,與病例對照組比較差異有統計學意義(P＜0.05),與正常對照組比較差異無統計學意義(P＞0.05).結論 MPP患兒外週血淋巴細胞中CD4+ CD25+ Treg細胞、CD3+T細胞錶達明顯受抑,CD4+/CD8+亞群比例異常,T細胞亞群數量及功能明顯紊亂,而體外試驗時維生素A體內活性代謝產物視黃痠榦預後能增彊淋巴細胞CD4+ CD25+ Treg細胞、CD3+ T細胞錶達,恢複CD4+/CD8+亞群比例,糾正T細胞亞群功能失衡,從而增彊、協調機體免疫、抗感染能力,為維生素A輔助治療MPP提高參攷.
목적 탐토폐염지원체폐염(MPP)환인외주혈림파세포중CD4+ CD25+조절성T세포(Treg)、T세포아군CD3+、CD4+、CD4+/CDs수평적변화급림상의의;탐토체외실험시유생소A체내활성대사산물시황산대MPP환인외주혈림파세포CD4+ CD25+ Treg세포、T세포아군CD3+、CD4+표체급CD4+/CD8+비솔적조절간예작용.방법 선택2012년10-12월재공군총의원인과학진위MPP적20례주원환인작위연구대상,기중10례환인작위병례시험조,령10례환인작위병례대조조(즉미여시황산부육),동기10례동령건강인동작위정상대조조.수집환인급정상대조인동외주혈표본,분리단개핵세포,용류식세포의검측외주혈림파세포중CD4+ CD25+ Treg세포、T세포아군CD3+、CD4+、CD4+/CD8+수평,병례시험조제취기림파세포재체외배양기중가입일정량시황산공동배양부육,재차측정CD4+ CD25+ Treg세포、T세포아군CD3+、CD4+、CD4+/CD8+수평,장상술지표진행비교분석.결과 병례조환인외주혈림파세포중CD4+ CD25+ Treg세포、CD4+/CD8+、CD3+백분비분별위(4.5±1.9)％、(1.3±0.9)％、(38.9±11.4)％,정상대조조인동분별위(13.2±2.5)％、(7.9±3.0)％、(65.1±8.8)％,병례대조조명현저우정상대조조,차이유통계학의의(P＜0.05),이여시황산공동부육후병례시험조이상지표명현승고,분별상승지(9.2±3.8)％、(5.9±2.5)％、(81.3±11.6)％,여병례대조조비교차이유통계학의의(P＜0.05),여정상대조조비교차이무통계학의의(P＞0.05).결론 MPP환인외주혈림파세포중CD4+ CD25+ Treg세포、CD3+T세포표체명현수억,CD4+/CD8+아군비례이상,T세포아군수량급공능명현문란,이체외시험시유생소A체내활성대사산물시황산간예후능증강림파세포CD4+ CD25+ Treg세포、CD3+ T세포표체,회복CD4+/CD8+아군비례,규정T세포아군공능실형,종이증강、협조궤체면역、항감염능력,위유생소A보조치료MPP제고삼고.
Objective To explore the levels of CD4+,CD25+,regulatory T cells (Treg),T cell subgroup CD3+,CD4+,CD4+/CDs+ of lymphocytes in peripheral blood of children with mycoplasma pneumoniae pneumonia (MPP) ; to observe the effect of retinoic acid (RA) and the metabolic product of vitamin A(VA) in vivo.Methods A total of 20 children with confirmed MPP were as case group,and l0 healthy children were as control group,from October 2012 to December 2012.The patients were divided into case observation group (RA,retinoic acid-the metabolic product of VA in vitro) and case control group (no RA),with 10 patients in each group.10 children were in the control group.The T lymphocytes were isolated from peripheral blood mononuclear cells (PBMC) among 20 patients with MPP and 10 healthy children; the levels of CD4+,CD225+,regulatory T cells,CD3+,CD4+,CD4+/CDs+ were measured by flow cytometry (FCM) for both groups.Results The ratio of CD4+ CD25+ Treg,CD4+ / CDs+,CD3+ from MPP children were (4.5 ± 1.9) ％,(1.3 ± 0.9) ％,(38.9 ± 11.4) ％ respectively,and the ratio of healthy children were (13.2 ± 2.5) ％,(7.9 ± 3.0) ％,(65.1 ± 8.8) ％ respectively.The rate of MPP children was lower that of healthy children,but there were no significant differences (P ＜ 0.05).The rate of CD4+,CD25+,Treg,CD4+/CD8+,CD3+ in MPP children were(9.2 ±3.8)％,(5.9 ±2.5)％,(81.3 ± 11.6)％ after RAintervention,higher than those in case control group (no RA) and there were significant differences (P ＜ 0.05).There were no significant differences between RA intervention group and healthy control group (P ＞ 0.05).Conclusion The suppression of CD4+,CD25+,Treg,CD3+ and the ratio of CD4+/CD8+,the dis-function of T cell are significant in children with MPP.
