中国医药
中國醫藥
중국의약
CHINA MEDICINE
2015年
1期
122-125
,共4页
刘桂兰%曹峰林%赵铭宇%石晶%刘树辉
劉桂蘭%曹峰林%趙銘宇%石晶%劉樹輝
류계란%조봉림%조명우%석정%류수휘
外阴硬化性苔癣%杀伤细胞免疫球蛋白受体基因%多态性
外陰硬化性苔癬%殺傷細胞免疫毬蛋白受體基因%多態性
외음경화성태선%살상세포면역구단백수체기인%다태성
Vulvar lichen sclerosus%Killer cell immunoglobulin-like receptor gene%Polymorphisms
目的 探讨肝肾阴虚型外阴硬化性苔癣(VLS)与杀伤细胞免疫球蛋白样受体(KIR)基因多态性的相关性.方法 选择30例肝肾阴虚型外阴硬化性苔癣患者作为试验组,30例体检健康女性为对照组.取受试者外周静脉血提取出人类基因组DNA,采用序列特异性引物聚合酶链反应(PCR-SSP)的方法,扩增出KIR基因的14个基因型.结果 30例VLS患者和30例健康对照个体中共检出了14个KIR基因,除框架基因KIR2DL4、KIR3DL2和KIR3DL3之外,KIR3DL1、KIR2DL3基因的表现型频率也是100%,KIR2DL1基因的表现型频率大于90%;其余依次为KIR2DL5、KIR2DS2、KIR2DS4、KIR2DS5的表现型频率在30% ~ 70%之间;表现型频率较低者为KIR2DL2、KIR2DS1、KIR2DS3、KIR3DS1,分别为6.7%、0.0%、3.3%、13.3%.其中,实验组KIR2DL2基因和KIR3DS1基因与对照组比较差异有统计学意义(P<0.01),其基因频率分别是0.03和0.07,而其他12个基因与对照组比较差异均无统计学意义(P>0.05).结论 KIR基因多态性与肝肾阴虚型VLS的发病有一定的相关性,抑制性基因KIR2DL2和激活性基因KIR3DS1可能是本病的拮抗性基因,其差异性表达可能在肝肾阴虚型VLS的发病发挥作用.
目的 探討肝腎陰虛型外陰硬化性苔癬(VLS)與殺傷細胞免疫毬蛋白樣受體(KIR)基因多態性的相關性.方法 選擇30例肝腎陰虛型外陰硬化性苔癬患者作為試驗組,30例體檢健康女性為對照組.取受試者外週靜脈血提取齣人類基因組DNA,採用序列特異性引物聚閤酶鏈反應(PCR-SSP)的方法,擴增齣KIR基因的14箇基因型.結果 30例VLS患者和30例健康對照箇體中共檢齣瞭14箇KIR基因,除框架基因KIR2DL4、KIR3DL2和KIR3DL3之外,KIR3DL1、KIR2DL3基因的錶現型頻率也是100%,KIR2DL1基因的錶現型頻率大于90%;其餘依次為KIR2DL5、KIR2DS2、KIR2DS4、KIR2DS5的錶現型頻率在30% ~ 70%之間;錶現型頻率較低者為KIR2DL2、KIR2DS1、KIR2DS3、KIR3DS1,分彆為6.7%、0.0%、3.3%、13.3%.其中,實驗組KIR2DL2基因和KIR3DS1基因與對照組比較差異有統計學意義(P<0.01),其基因頻率分彆是0.03和0.07,而其他12箇基因與對照組比較差異均無統計學意義(P>0.05).結論 KIR基因多態性與肝腎陰虛型VLS的髮病有一定的相關性,抑製性基因KIR2DL2和激活性基因KIR3DS1可能是本病的拮抗性基因,其差異性錶達可能在肝腎陰虛型VLS的髮病髮揮作用.
목적 탐토간신음허형외음경화성태선(VLS)여살상세포면역구단백양수체(KIR)기인다태성적상관성.방법 선택30례간신음허형외음경화성태선환자작위시험조,30례체검건강녀성위대조조.취수시자외주정맥혈제취출인류기인조DNA,채용서렬특이성인물취합매련반응(PCR-SSP)적방법,확증출KIR기인적14개기인형.결과 30례VLS환자화30례건강대조개체중공검출료14개KIR기인,제광가기인KIR2DL4、KIR3DL2화KIR3DL3지외,KIR3DL1、KIR2DL3기인적표현형빈솔야시100%,KIR2DL1기인적표현형빈솔대우90%;기여의차위KIR2DL5、KIR2DS2、KIR2DS4、KIR2DS5적표현형빈솔재30% ~ 70%지간;표현형빈솔교저자위KIR2DL2、KIR2DS1、KIR2DS3、KIR3DS1,분별위6.7%、0.0%、3.3%、13.3%.기중,실험조KIR2DL2기인화KIR3DS1기인여대조조비교차이유통계학의의(P<0.01),기기인빈솔분별시0.03화0.07,이기타12개기인여대조조비교차이균무통계학의의(P>0.05).결론 KIR기인다태성여간신음허형VLS적발병유일정적상관성,억제성기인KIR2DL2화격활성기인KIR3DS1가능시본병적길항성기인,기차이성표체가능재간신음허형VLS적발병발휘작용.
Objective To study the correlation among vulvar lichen sclerosus(VLS),liver and kidneyyindeficiency type and killer cell immunoglobulin-like receptor (KIR) gene polymorphism.Methods Totally 30 patients with liver and kidney indeficiency were as experimental group and the 30 healthy women were as control group.Mter the human genomic DNA of human peripheral blood leukocytes was tested; 14 polymorphisms of KIR gene were amplified by sequence-specific primer polymerase chain reaction (PCR-SSP) method.Results We detected all the 14 KIR gene polymorphisms in 30 VLS cases and 30 controls.Besides frame gene,phenotype frequency of KIR2DL3,KIR3DL1 gene was also 100% ; phenotype frequency of KIR2DLl was >90%,followed by KIR2DL5,KIR2DS2,KIR2DS4,KIR2DS5 phenotype frequencies.Among them,KIR2DL2 and KIR3DS1 had significant differences between twogroups(P <0.01) ; the gene frequencies were 0.03 and 0.07.There were no significant difference amongother 12 genes (P > 0.05).Conclusions There is a correlation between KIR gene polymorphisms and the onset of the liver and kidney yin deficiency type VLS' inhibitory gene KIR2DL2 ;the activated gene KIR3DS1 may be the antagonistic gene of VLS.The gene differential expression may play a role in the pathogenesis of liver and kidney yin deficiency type VLS.