CAJ | 학술논문

目的评价程序性坏死在大鼠肠缺血再灌注损伤中的作用.方法健康雄性SD大鼠32只,体重200～220 g,采用随机数字表法,将其分为4组(n=8):假手术组(Sham组)、肠缺血再灌注组(I/R组)、程序性坏死特异性抑制剂Necrostatin-1组(Nec-1组)和溶剂二甲基亚砜组(DMSO组).采用夹闭肠系膜上动脉1h再灌注24 h的方法制备肠缺血再灌注损伤模型.Sham组仅分离血管;Nec-1组及DMSO组分别于夹闭肠系膜上动脉前30 min时腹腔注射Necrostatin-1 1.0 mg/kg或等容量二甲基亚砜.于再灌注24 h时取肠组织,观察肠粘膜形态学并行Chiu评分,开胸经心尖取血样2ml,检测血清二胺氧化酶(DAO)活性.采用Western blot法检测肠粘膜组织活化的cmpase-3蛋白和受体相互作用蛋白1(RIP1)的表达水平.结果与Sham组比较,I/R组、DMSO组及Nec-1组Chiu评分和血清DAO活性升高,肠粘膜组织活化的caspase-3蛋白和RIP1蛋白表达上调(P＜0.01);与I/R组和DMSO组比较,Nec-1组Chiu评分和血清DAO活性降低,肠粘膜组织RIP1蛋白表达下调(P＜0.05),活化的caspase-3蛋白表达差异无统计学意义(P＞0.05).结论程序性坏死参与了大鼠肠缺血再灌注损伤.
목적 평개정서성배사재대서장결혈재관주손상중적작용.방법 건강웅성SD대서32지,체중200～220 g,채용수궤수자표법,장기분위4조(n=8):가수술조(Sham조)、장결혈재관주조(I/R조)、정서성배사특이성억제제Necrostatin-1조(Nec-1조)화용제이갑기아풍조(DMSO조).채용협폐장계막상동맥1h재관주24 h적방법제비장결혈재관주손상모형.Sham조부분리혈관;Nec-1조급DMSO조분별우협폐장계막상동맥전30 min시복강주사Necrostatin-1 1.0 mg/kg혹등용량이갑기아풍.우재관주24 h시취장조직,관찰장점막형태학병행Chiu평분,개흉경심첨취혈양2ml,검측혈청이알양화매(DAO)활성.채용Western blot법검측장점막조직활화적cmpase-3단백화수체상호작용단백1(RIP1)적표체수평.결과 여Sham조비교,I/R조、DMSO조급Nec-1조Chiu평분화혈청DAO활성승고,장점막조직활화적caspase-3단백화RIP1단백표체상조(P＜0.01);여I/R조화DMSO조비교,Nec-1조Chiu평분화혈청DAO활성강저,장점막조직RIP1단백표체하조(P＜0.05),활화적caspase-3단백표체차이무통계학의의(P＞0.05).결론 정서성배사삼여료대서장결혈재관주손상.
Objective To evaluate the role of necroptosis in intestinal ischemia-reperfusion (I/R) injury in rats.Methods Thirty-two healthy male Sprague-Dawley rats,weighing 200-220 g,were randomly assigned into 4 groups (n =.8 each) using a random number table:sham operation group (Sham group),I/R group,necroptosis inhibitor necrostatin-1 group (Nec-1 group) and solvent dimethyl sulfoxide (DMSO) group (group DMSO).Intestinal I/R injury was produced by clamping the superior mesenteric artery for 1 h followed by 24 h reperfusion in rats anesthetized with chloral hydrate.Necrostatin-1 1.0 mg/kg was administered intraperitoneally at 30 min before occlusion in Nec-1 group,while the equal volume of DMSO was given instead in group DMSO.The rats were sacrificed at 24 h of reperfusion and the intestinal tissues were removed for microscopic examination.Intestinal damage was assessed and scored according to Chiu.Blood samples were taken for determination of serum diamine oxidase (DAO) activity.The expression of activitied caspase-3 and receptor-interacting protein 1 (RIP1) in intestinal tissues was detected using Western blot.Results Compared with Sham group,Chiu's score,serum DAO activity,and the expression of activitied caspase-3 and RIP1 was up-regulated in I/R,DMSO and Nec-1 groups.Compared with I/R and DMSO groups,Chiu's score and DAO activity were significantly decreased,the expression of RIP1 was down-regulated,and no significant change was found in the expression of activitied caspase3 in group Nec-1.Conclusion Necroptosis is involved in intestinal I/R injury in rats.