CAJ | 학술논문

目的评价外源性硫化氢(H2S)对大鼠肾缺血再灌注诱发心肌损伤的影响.方法清洁级雄性Wistar大鼠30只,体重250～300g,采用随机数字表法分为3组(n=l0):假手术组(S组)、肾缺血再灌注诱发心肌损伤组(I/R组)和NaHS组.采用肾缺血120 min后恢复灌注60 min的方法制备大鼠肾缺血再灌注诱发心肌损伤模型.NaHS组于再灌注前20 min时腹腔注射NaHS 100 μg/kg.于再灌注结束即刻处死大鼠取心肌组织,采用硫代巴比妥酸法检测丙二醛(MDA)含量,二硫二硝基苯甲酸法检测谷胱甘肽过氧化物酶(GSH-Px)活性,光镜下观察病理学结果.结果与S组比较,I/R组和NaHS组心肌MDA含量升高,GSH-Px活性降低(P＜0.05);与I/R组比较,NaHS组心肌MDA含量降低,GSH-Px活性升高(P＜0.05).NaHS组心肌病理学损伤较I/R组明显减轻.结论外源性H2S可减轻大鼠肾缺血再灌注诱发的心肌损伤.
목적 평개외원성류화경(H2S)대대서신결혈재관주유발심기손상적영향.방법 청길급웅성Wistar대서30지,체중250～300g,채용수궤수자표법분위3조(n=l0):가수술조(S조)、신결혈재관주유발심기손상조(I/R조)화NaHS조.채용신결혈120 min후회복관주60 min적방법제비대서신결혈재관주유발심기손상모형.NaHS조우재관주전20 min시복강주사NaHS 100 μg/kg.우재관주결속즉각처사대서취심기조직,채용류대파비타산법검측병이철(MDA)함량,이류이초기분갑산법검측곡광감태과양화물매(GSH-Px)활성,광경하관찰병이학결과.결과 여S조비교,I/R조화NaHS조심기MDA함량승고,GSH-Px활성강저(P＜0.05);여I/R조비교,NaHS조심기MDA함량강저,GSH-Px활성승고(P＜0.05).NaHS조심기병이학손상교I/R조명현감경.결론 외원성H2S가감경대서신결혈재관주유발적심기손상.
Objective To evaluate the effect of exogenous hydrogen sulfide (H2 S) on myocardial damage induced by renal ischemia-reperfusion (I/R) in rats.Methods Thirty pathogen-free male Wistar rats,weighing 250-300 g,were randomized into 3 groups (n =10 each) using a random number table:sham operation group (S group); myocardial damage induced by renal I/R group (I/R group); NaHS group.Renal I/R was induced by occlusion of the left kidney for 120 min followed by 60 min reperfusion in anesthetized rats.In NaHS group,NaHS 100 μg/kg was injected intraperitoneally at 20 min before reperfusion.At the end of reperfusion,the rats were sacrificed and the hearts were obtained for determination of malondialdehyde (MDA) content and glutathione peroxidase (GSH-Px) activity in myocardial tissues and for microscopic examination.Results Compared with S group,MDA content was significantly increased,and GSH-Px activity was decreased in I/R and NaHS groups.Compared with I/R group,MDA content was significantly decreased,and GSH-Px activity was increased in NaHS group.The pathological changes of myocardium were significantly attenuated in NaHS group as compared with I/R group.Conclusion Exogenous H2 S can alleviate myocardial damage induced by renal I/R in rats.