中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2015年
2期
126-128
,共3页
巴剑波%王明科%武文斌%徐雄利%陈海庭%陈锐勇
巴劍波%王明科%武文斌%徐雄利%陳海庭%陳銳勇
파검파%왕명과%무문빈%서웅리%진해정%진예용
潜水%白细胞介素6%白细胞介素10%肿瘤坏死因子α
潛水%白細胞介素6%白細胞介素10%腫瘤壞死因子α
잠수%백세포개소6%백세포개소10%종류배사인자α
Diving%IL-6%IL-10%TNF-α
目的 探讨模拟50 m水深氮氧饱和暴露对兔外耳道炎症因子白细胞介素6(IL-6)、IL-10和肿瘤坏死因子α(TNF-α)表达的影响.方法 新西兰兔分2批于50 m水深氮氧饱和暴露,饱和暴露后取外耳道黏膜上皮组织进行HE染色.应用实时聚合酶链式反应(Real-time PCR)检测兔外耳道黏膜上皮炎症因子IL-6、IL-10和TNF-α表达的变化.结果 HE染色显示,50 m水深氮氧饱和暴露组较对照组兔外耳道黏膜上皮炎症细胞浸润增加,小血管充血及黏膜水肿明显.Real-time PCR检测显示饱和暴露组较对照组兔外耳道黏膜上皮IL-6、TNF-α表达增加,而IL-10表达降低.结论 50 m水深氮氧饱和暴露可致兔外耳道黏膜上皮炎症损伤,机制可能与IL-6、TNF-α表达增加,而IL-10表达降低有关.
目的 探討模擬50 m水深氮氧飽和暴露對兔外耳道炎癥因子白細胞介素6(IL-6)、IL-10和腫瘤壞死因子α(TNF-α)錶達的影響.方法 新西蘭兔分2批于50 m水深氮氧飽和暴露,飽和暴露後取外耳道黏膜上皮組織進行HE染色.應用實時聚閤酶鏈式反應(Real-time PCR)檢測兔外耳道黏膜上皮炎癥因子IL-6、IL-10和TNF-α錶達的變化.結果 HE染色顯示,50 m水深氮氧飽和暴露組較對照組兔外耳道黏膜上皮炎癥細胞浸潤增加,小血管充血及黏膜水腫明顯.Real-time PCR檢測顯示飽和暴露組較對照組兔外耳道黏膜上皮IL-6、TNF-α錶達增加,而IL-10錶達降低.結論 50 m水深氮氧飽和暴露可緻兔外耳道黏膜上皮炎癥損傷,機製可能與IL-6、TNF-α錶達增加,而IL-10錶達降低有關.
목적 탐토모의50 m수심담양포화폭로대토외이도염증인자백세포개소6(IL-6)、IL-10화종류배사인자α(TNF-α)표체적영향.방법 신서란토분2비우50 m수심담양포화폭로,포화폭로후취외이도점막상피조직진행HE염색.응용실시취합매련식반응(Real-time PCR)검측토외이도점막상피염증인자IL-6、IL-10화TNF-α표체적변화.결과 HE염색현시,50 m수심담양포화폭로조교대조조토외이도점막상피염증세포침윤증가,소혈관충혈급점막수종명현.Real-time PCR검측현시포화폭로조교대조조토외이도점막상피IL-6、TNF-α표체증가,이IL-10표체강저.결론 50 m수심담양포화폭로가치토외이도점막상피염증손상,궤제가능여IL-6、TNF-α표체증가,이IL-10표체강저유관.
Objective To investigate the effects of simulated nitrogen-oxygen saturation exposure at a water depth of 50 m on the expression of inflammatory mediators including interleukin-6 (IL-6),interleukin-10 (IL-10),and tumor necrosis factor-alpha (TNF-α) in the external auditory canal (EAC) of rabbits.Methods Two batches of New Zealand rabbits were exposed to nitrogen-oxygen saturated at a water depth of 50 m.After exposure,the epithelial tissue in the EAC was analyzed using hematoxylin-eosin (HE) staining,and the changes in expression of inflammatory mediators including IL-6,IL-10,and TNF-α in the EAC of rabbits were determined by real-time polymerase chain reaction (PCR).Results According to the result of HE staining,more inflammatory cell infiltration,small vascular congestion,and mucosal edema in the EAC of rabbits were observed in the exposure group than in the control group.Additionally,compared with the control group,the exposure group had increased expression of IL-6 and TNF-α and reduced expression of IL-10 in the EAC of rabbits according to the result of real-time PCR.Conclusion The nitrogen-oxygen saturation exposure at a water depth of 50 m can cause inflammatory injuries in the EAC of rabbits.The mechanism may be associated with increased expression of IL-6 and TNF-α and reduced expression of IL-10.
