中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2015年
1期
11-14
,共4页
晋乐飞%冯斐斐%李冰%燕贞%梁宁%杨维超%刘文佳%吴卫东
晉樂飛%馮斐斐%李冰%燕貞%樑寧%楊維超%劉文佳%吳衛東
진악비%풍비비%리빙%연정%량저%양유초%류문가%오위동
纳米%氧化锌%上皮细胞%血红素氧合酶-1%血小板内皮细胞黏附分子-1
納米%氧化鋅%上皮細胞%血紅素氧閤酶-1%血小闆內皮細胞黏附分子-1
납미%양화자%상피세포%혈홍소양합매-1%혈소판내피세포점부분자-1
Nanoparticles%Zinc oxide%Epithelial cells%Heme oxygenase-1%Platelet endothelial cell adhesion molecules-1
目的 探讨纳米氧化锌(zinc oxide nanoparticles,ZnO-NPs)对人冠状动脉内皮细胞血红素氧合酶-1(heme oxygenase-1,HO-1)和血小板内皮细胞黏附分子-1(platelet endothelial cell adhesion molecules-1,PECAM-1)蛋白表达的影响.方法 采用噻唑蓝实验(MTT比色法)检测ZnO-NPs(12.5、25、50、70、80、90、100、200 μg/ml处理后)对人冠状动脉内皮细胞存活率的影响.用不同浓度ZnO-NPs(0、10、20、40 μg/ml)刺激人冠状动脉内皮细胞24 h后,用ELISA法检测细胞培养上清中HO-1及PECAM-1蛋白的表达.结果 12.5、25、50、70、80、90、100、200 μg/ml ZnO-NPs处理后,人冠状动脉内皮细胞的存活率分别为89.76％、83.61％、63.10％、53.20％、48.11％、42.35％、38.06％、25.44％.0、10、20、40μg/ml ZnO-NPs刺激后,人冠状动脉内皮细胞HO-1蛋白的表达量分别为(0.041±0.011)、(0.512±0.076)、(0.906±0.059)、(1.062±0.089) ng/L,两两比较,差异均有统计学意义(P＜0.05),ZnO-NPs浓度与HO-1蛋白表达存在剂量-效应关系.0、10、20、40 μg/ml ZnO-NPs刺激后,人冠状动脉内皮细胞PECAM-1蛋白的表达量分别为(7.966±0.046)、(7.993±0.036)、(8.629±0.052)、(8.811±0.039) μg/L,当ZnO-NPs浓度为20、40 μg/ml时,与空白对照组比较,PECAM-1蛋白的表达均上升,差异均有统计学意义(P＜0.05).结论 ZnO-NPs能够降低人冠状动脉内皮细胞存活率,并可诱导人冠状动脉内皮细胞HO-1和PECAM-1蛋白表达上调.
目的 探討納米氧化鋅(zinc oxide nanoparticles,ZnO-NPs)對人冠狀動脈內皮細胞血紅素氧閤酶-1(heme oxygenase-1,HO-1)和血小闆內皮細胞黏附分子-1(platelet endothelial cell adhesion molecules-1,PECAM-1)蛋白錶達的影響.方法 採用噻唑藍實驗(MTT比色法)檢測ZnO-NPs(12.5、25、50、70、80、90、100、200 μg/ml處理後)對人冠狀動脈內皮細胞存活率的影響.用不同濃度ZnO-NPs(0、10、20、40 μg/ml)刺激人冠狀動脈內皮細胞24 h後,用ELISA法檢測細胞培養上清中HO-1及PECAM-1蛋白的錶達.結果 12.5、25、50、70、80、90、100、200 μg/ml ZnO-NPs處理後,人冠狀動脈內皮細胞的存活率分彆為89.76％、83.61％、63.10％、53.20％、48.11％、42.35％、38.06％、25.44％.0、10、20、40μg/ml ZnO-NPs刺激後,人冠狀動脈內皮細胞HO-1蛋白的錶達量分彆為(0.041±0.011)、(0.512±0.076)、(0.906±0.059)、(1.062±0.089) ng/L,兩兩比較,差異均有統計學意義(P＜0.05),ZnO-NPs濃度與HO-1蛋白錶達存在劑量-效應關繫.0、10、20、40 μg/ml ZnO-NPs刺激後,人冠狀動脈內皮細胞PECAM-1蛋白的錶達量分彆為(7.966±0.046)、(7.993±0.036)、(8.629±0.052)、(8.811±0.039) μg/L,噹ZnO-NPs濃度為20、40 μg/ml時,與空白對照組比較,PECAM-1蛋白的錶達均上升,差異均有統計學意義(P＜0.05).結論 ZnO-NPs能夠降低人冠狀動脈內皮細胞存活率,併可誘導人冠狀動脈內皮細胞HO-1和PECAM-1蛋白錶達上調.
목적 탐토납미양화자(zinc oxide nanoparticles,ZnO-NPs)대인관상동맥내피세포혈홍소양합매-1(heme oxygenase-1,HO-1)화혈소판내피세포점부분자-1(platelet endothelial cell adhesion molecules-1,PECAM-1)단백표체적영향.방법 채용새서람실험(MTT비색법)검측ZnO-NPs(12.5、25、50、70、80、90、100、200 μg/ml처리후)대인관상동맥내피세포존활솔적영향.용불동농도ZnO-NPs(0、10、20、40 μg/ml)자격인관상동맥내피세포24 h후,용ELISA법검측세포배양상청중HO-1급PECAM-1단백적표체.결과 12.5、25、50、70、80、90、100、200 μg/ml ZnO-NPs처리후,인관상동맥내피세포적존활솔분별위89.76％、83.61％、63.10％、53.20％、48.11％、42.35％、38.06％、25.44％.0、10、20、40μg/ml ZnO-NPs자격후,인관상동맥내피세포HO-1단백적표체량분별위(0.041±0.011)、(0.512±0.076)、(0.906±0.059)、(1.062±0.089) ng/L,량량비교,차이균유통계학의의(P＜0.05),ZnO-NPs농도여HO-1단백표체존재제량-효응관계.0、10、20、40 μg/ml ZnO-NPs자격후,인관상동맥내피세포PECAM-1단백적표체량분별위(7.966±0.046)、(7.993±0.036)、(8.629±0.052)、(8.811±0.039) μg/L,당ZnO-NPs농도위20、40 μg/ml시,여공백대조조비교,PECAM-1단백적표체균상승,차이균유통계학의의(P＜0.05).결론 ZnO-NPs능구강저인관상동맥내피세포존활솔,병가유도인관상동맥내피세포HO-1화PECAM-1단백표체상조.
Objective To explore the protein expression of heme oxygenase-1 (HO-1) and platelet endothelial cell adhesion molecules-1 (PECAM-1) in human coronary artery endothelial cells induced with Zinc Oxide Nanoparticle (ZnO-NPs).Methods MTT assay was used to determine the cell viability of ZnO-NPs.Levels of HO-1 and PECAM-1 protein in culture supernatants were measured using ELISA after human coronary artery endothelial cells were treated with different concentrations (0,10,20,40μg/ml) of ZnO-NPs for 24 h.Results The cell viability of human coronary artery endothelial cells in each group was 89.76％,83.61％,63.10％,53.20％,48.11％,42.35％,38.06％,25.44％ respectively when treated with different concentrations of ZnO-NPs (12.5,25,50,70,80,90,100,200μg/ml).Protein levels of HO-1 (ng/L) in each group were 0.041±0.011,0.512±0.076,0.906±0.059,1.062±0.089 respectively after the stimulation of different concentrations of ZnO-NPs (0,10,20,40μg/ml).Comparisons in each group were statistically significant (P＜0.05).Protein levels of PECAM-1 (μg/L) in each group were 7.966±0.046,7.993±0.036,8.629±0.052,8.811 ±0.039respectively after the stimulation of different concentrations of ZnO-NPs (0,10,20,40 μg/ml).Compared with the control group,protein levels of PECAM-1 increased (P＜0.05) when the concentration of ZnO-NPs was 20 μg/ml or 40 μg/ml.Conclusion ZnO-NPs stimulation could inhibit the viability of human coronary artery endothelial cells and upregulate the protein expression of HO-1 and PECAM-1.
